Human Wilms tumor protein 1-interacting protein ELISA Kit | WTIP elisa kit

Human Wilms tumor protein 1-interacting protein ELISA Kit

Cat. Num. AAA761931

• Reactivity: Human
• Synonyms: Wilms tumor protein 1-interacting protein; Human Wilms tumor protein 1-interacting protein ELISA Kit; WTIP (Wilms tumor protein 1-interacting protein)/WT1-interacting protein; WTIP elisa kit

• Reactivity: Human
• Sequence Length: 430

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Sandwich ELISA, Double Antibody
• Detection Range: 0.39-25ng/ml
• Sensitivity: 0.234ng/ml
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data

Typical Standard Curve

Related Product Information for WTIP elisa kit

Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. anti-Human WTIP antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Human WTIP antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human WTIP amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Human WTIP can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 122937269
• NCBI GeneID: 126374
• NCBI Accession #: NP_001073905.1
• NCBI GenBank Nucleotide #: NM_001080436.1
• Molecular Weight: 45,124 Da
• NCBI Official Full Name: Wilms tumor protein 1-interacting protein
• NCBI Official Synonym Full Names: Wilms tumor 1 interacting protein
• NCBI Official Symbol: WTIP
• NCBI Protein Information: Wilms tumor protein 1-interacting protein
• UniProt Protein Name: Wilms tumor protein 1-interacting protein
• Protein Family: Wilms tumor protein 1-interacting protein
• UniProt Gene Name: WTIP
• UniProt Synonym Gene Names: WT1-interacting protein
• UniProt Entry Name: WTIP_HUMAN

Uniprot Description

WTIP: Adapter or scaffold protein which participates in the assembly of numerous protein complexes and is involved in several cellular processes such as cell fate determination, cytoskeletal organization, repression of gene transcription, cell-cell adhesion, cell differentiation, proliferation and migration. Positively regulates microRNA (miRNA)-mediated gene silencing. Negatively regulates Hippo signaling pathway and antagonizes phosphorylation of YAP1. Acts as a transcriptional corepressor for SNAI1 and SNAI2/SLUG-dependent repression of E-cadherin transcription. Acts as a hypoxic regulator by bridging an association between the prolyl hydroxylases and VHL enabling efficient degradation of HIF1A. In podocytes, may play a role in the regulation of actin dynamics and/or foot process cytoarchitecture (By similarity). In the course of podocyte injury, shuttles into the nucleus and acts as a transcription regulator that represses WT1-dependent transcription regulation, thereby translating changes in slit diaphragm structure into altered gene expression and a less differentiated phenotype

Protein type: Transcription, coactivator/corepressor; Cell development/differentiation; Adaptor/scaffold

Chromosomal Location of Human Ortholog: 19q13.11

Cellular Component: adherens junction; nucleus

Molecular Function: protein binding; transcription corepressor activity; zinc ion binding

Biological Process: cytoskeleton organization and biogenesis; miRNA-mediated gene silencing; regulation of cell morphogenesis; regulation of transcription, DNA-dependent; response to hypoxia; transcription, DNA-dependent