Human Metalloreductase STEAP1 ELISA Kit | STEAP1 elisa kit

Human Metalloreductase STEAP1 ELISA Kit

Cat. Num. AAA760856

• Gene Names: STEAP1; STEAP; PRSS24
• Reactivity: Human
• Synonyms: Metalloreductase STEAP1; Human Metalloreductase STEAP1 ELISA Kit; STEAP1 (Metalloreductase STEAP1)/PRSS24/STEAP/six transmembrane epithelial antigen of the prostate/Six-transmembrane epithelial antigen of prostate 1; STEAP1 elisa kit

• Reactivity: Human
• Sequence Length: 339

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Sandwich ELISA, Double Antibody
• Detection Range: 0.39-25ng/ml
• Sensitivity: 0.234ng/ml
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data

Typical Standard Curve

Related Product Information for STEAP1 elisa kit

Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-Human STEAP1 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Human STEAP1 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human STEAP1 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Human STEAP1 can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 9558759
• NCBI GeneID: 26872
• NCBI Accession #: NP_036581.1
• NCBI GenBank Nucleotide #: NM_012449.2
• Molecular Weight: 39,851 Da
• NCBI Official Full Name: metalloreductase STEAP1
• NCBI Official Synonym Full Names: six transmembrane epithelial antigen of the prostate 1
• NCBI Official Symbol: STEAP1
• NCBI Official Synonym Symbols: STEAP; PRSS24
• NCBI Protein Information: metalloreductase STEAP1
• UniProt Protein Name: Metalloreductase STEAP1
• Protein Family: Metalloreductase
• UniProt Gene Name: STEAP1
• UniProt Synonym Gene Names: PRSS24; STEAP
• UniProt Entry Name: STEA1_HUMAN

NCBI Description

This gene is predominantly expressed in prostate tissue, and is found to be upregulated in multiple cancer cell lines. The gene product is predicted to be a six-transmembrane protein, and was shown to be a cell surface antigen significantly expressed at cell-cell junctions. [provided by RefSeq, Jul 2008]

Uniprot Description

STEAP1: Metalloreductase that has the ability to reduce both Fe(3+) to Fe(2+) and Cu(2+) to Cu(1+). Uses NAD(+) as acceptor. Belongs to the STEAP family.

Protein type: EC 1.16.1.-; Membrane protein, multi-pass; Oxidoreductase; Cell adhesion; Membrane protein, integral

Chromosomal Location of Human Ortholog: 7q21

Cellular Component: endosome membrane; integral to plasma membrane; intercellular junction; membrane; plasma membrane

Molecular Function: channel activity; cupric reductase activity; metal ion binding; transporter activity

Biological Process: copper ion import; iron ion homeostasis; transmembrane transport