Human Wilms tumor protein ELISA Kit | WT1 elisa kit

Human Wilms tumor protein ELISA Kit

Cat. Num. AAA761090

• Gene Names: WT1; GUD; AWT1; WAGR; WT33; NPHS4; WIT-2; EWS-WT1
• Reactivity: Human
• Synonyms: Wilms tumor protein; Human Wilms tumor protein ELISA Kit; WT1/Wilms tumor protein/GUD/WAGR/WIT-2/WT33/AWT1/NPHS4GUD/WAGR/Wilms tumor 1; WT1 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of WNT5A. No significant cross-reactivity or interference between WNT5A and analogues was observed.
• Sequence Length: 345

• Samples: Serum, Plasma, Tissue Homogenates And Other Biological Fluids
• Assay Type: Sandwich
• Detection Range: 0.156-10ng/ml
• Sensitivity: < 0.094ng/ml
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level WNT5Awere tested 20 times on one plate, respectively. Intra-Assay: CV<8%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level WNT5Awere tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100. Inter-Assay: CV<10%
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for WT1 elisa kit

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-WNT5Aantibody was pre-coated onto 96-well plates. And the biotin conjugated anti-WNT5A antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the WNT5A amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of WNT5A can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 340382
• NCBI GeneID: 7490
• Molecular Weight: 33,086 Da
• NCBI Official Full Name: Wilms' tumor protein, partial
• NCBI Official Synonym Full Names: Wilms tumor 1
• NCBI Official Symbol: WT1
• NCBI Official Synonym Symbols: GUD; AWT1; WAGR; WT33; NPHS4; WIT-2; EWS-WT1
• NCBI Protein Information: Wilms tumor protein
• UniProt Protein Name: Wilms tumor protein
• Protein Family: Wilms tumor protein
• UniProt Gene Name: WT1
• UniProt Entry Name: WT1_HUMAN

NCBI Description

This gene encodes a transcription factor that contains four zinc-finger motifs at the C-terminus and a proline/glutamine-rich DNA-binding domain at the N-terminus. It has an essential role in the normal development of the urogenital system, and it is mutated in a small subset of patients with Wilms tumor. This gene exhibits complex tissue-specific and polymorphic imprinting pattern, with biallelic, and monoallelic expression from the maternal and paternal alleles in different tissues. Multiple transcript variants have been described. In several variants, there is evidence for the use of a non-AUG (CUG) translation initiation codon upstream of, and in-frame with the first AUG. Authors of PMID:7926762 also provide evidence that WT1 mRNA undergoes RNA editing in human and rat, and that this process is tissue-restricted and developmentally regulated. [provided by RefSeq, Mar 2015]

Uniprot Description

WT1: a DNA binding protein and apparent transcriptional regulator. Recognizes and binds to the DNA sequence 5'-CGCCCCCGC-3'. Expressed in the kidney and a subset of hematopoietic cells. Defects in WT1 are the cause of Wilms tumor 1 (WT1), an embryonal malignancy of the kidney that affects approximately 1 in 10'000 infants and young children. It occurs both in sporadic and hereditary forms. Four alternatively spliced isoforms have been described.

Protein type: C2H2-type zinc finger protein; DNA-binding; Nucleolus; Transcription factor; Tumor suppressor

Chromosomal Location of Human Ortholog: 11p13

Cellular Component: cytoplasm; nuclear speck; nucleus

Molecular Function: double-stranded methylated DNA binding; protein binding; sequence-specific DNA binding; transcription factor activity; zinc ion binding

Biological Process: adrenal gland development; camera-type eye development; epithelial cell differentiation; germ cell development; glomerular basement membrane development; glomerulus development; gonad development; heart development; kidney development; male genitalia development; male gonad development; negative regulation of apoptosis; negative regulation of cell growth; negative regulation of cell proliferation; negative regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent; positive regulation of transcription, DNA-dependent; regulation of transcription from RNA polymerase II promoter; regulation of transcription, DNA-dependent; RNA splicing; sex determination; thorax and anterior abdomen determination; tissue development; ureteric bud branching; ureteric bud development; vasculogenesis

Disease: Aniridia; Denys-drash Syndrome; Frasier Syndrome; Meacham Syndrome; Mesothelioma, Malignant; Nephrotic Syndrome, Type 4; Wilms Tumor 1; Wilms Tumor, Aniridia, Genitourinary Anomalies, And Mental Retardation Syndrome