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Immunofluorescence (Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0. 1% Triton X-100 permeabilized NIH/3T3 cells labeling Csf1r with AP13911B at 1/25 dilution, followed by Dylight® 488-conjugated goat anti-Rabbit IgG secondary antibody at 1/200 dilution (green). Immunofluorescence image showingCytoplasm staining on NIH/3T3 cell line. Cytoplasmic actin is detected with Dylight® 554 Phalloidin(red). The nuclear counter stain is DAPI (blue).)

Rabbit Csf1r Polyclonal Antibody | anti-Csf1r antibody

Mouse Csf1r Antibody (C-term)

Gene Names
Csf1r; Fms; CD115; Csfmr; Fim-2; CSF-1R; M-CSFR; M-CSF-R; AI323359
Reactivity
Mouse (Predicted Reactivity: Rat)
Applications
Western Blot, ELISA, Immunohistochemistry, Immunofluorescence
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
Csf1r; Polyclonal Antibody; Mouse Csf1r Antibody (C-term); Macrophage colony-stimulating factor 1 receptor; CSF-1 receptor; CSF-1-R; CSF-1R; M-CSF-R; Proto-oncogene c-Fms; CD115; Csfmr; Fms; anti-Csf1r antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Mouse (Predicted Reactivity: Rat)
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This Mouse Csf1r antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 895-923 amino acids from the C-terminal region of mouse Csf1r.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Sequence Positions
895-923
Sequence Length
977
Applicable Applications for anti-Csf1r antibody
Western Blot (WB), ELISA (EIA), Immunohistochemistry (IHC), Immunofluorescence (IF)
Application Notes
WB~~1:1000
IF~~1:25
IHC-P~~1:25
Antigen Type
Synthetic Peptide
Antigen Source
MOUSE
Function
Tyrosine-protein kinase that acts as cell-surface receptor for CSF1 and IL34 and plays an essential role in the regulation of survival, proliferation and differentiation of hematopoietic precursor cells, especially mononuclear phagocytes, such as macrophages and monocytes. Promotes the release of proinflammatory chemokines in response to IL34 and CSF1, and thereby plays an important role in innate immunity and in inflammatory processes. Plays an important role in the regulation of osteoclast proliferation and differentiation, the regulation of bone resorption, and is required for normal bone and tooth development. Required for normal male and female fertility, and for normal development of milk ducts and acinar structures in the mammary gland during pregnancy. Promotes reorganization of the actin cytoskeleton, regulates formation of membrane ruffles, cell adhesion and cell migration, and promotes cancer cell invasion. Activates several signaling pathways in response to ligand binding, including the ERK1/2 and the JNK pathway (By similarity). Phosphorylates PIK3R1, PLCG2, GRB2, SLA2 and CBL. Activation of PLCG2 leads to the production of the cellular signaling molecules diacylglyceroland inositol 1,4,5- trisphosphate, that thenlead to the activation of protein kinase Cfamily members, especially PRKCD.Phosphorylation of PIK3R1, the regulatorysubunit of phosphatidylinositol 3-kinase,leads to activation of the AKT1 signalingpathway. Activated CSF1R also mediates activation of the MAP kinases MAPK1/ERK2 and/or MAPK3/ERK1, and of the SRC family kinases SRC, FYN and YES1. Activated CSF1R transmits signals both via proteins that directly interact with phosphorylated tyrosine residues in its intracellular domain, or via adapter proteins, such as GRB2. Promotes activation of STAT family members STAT3, STAT5A and/or STAT5B. Promotes tyrosine phosphorylation of SHC1 and INPP5D/SHIP-1. Receptor signaling is down-regulated by protein phosphatases, such as INPP5D/SHIP-1, that dephosphorylate the receptor and its downstream effectors, and by rapid internalization of the activated receptor. In the central nervous system, may play a rolein the development of microglia macrophages (By similarity).
Cellular Location
Cell membrane; Single-pass type I membrane protein. Note=The autophosphorylated receptor is ubiquitinated and internalized, leading to its degradation
Tissue Location
Widely expressed..
Preparation and Storage
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Immunofluorescence

(Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0. 1% Triton X-100 permeabilized NIH/3T3 cells labeling Csf1r with AP13911B at 1/25 dilution, followed by Dylight® 488-conjugated goat anti-Rabbit IgG secondary antibody at 1/200 dilution (green). Immunofluorescence image showingCytoplasm staining on NIH/3T3 cell line. Cytoplasmic actin is detected with Dylight® 554 Phalloidin(red). The nuclear counter stain is DAPI (blue).)

Immunofluorescence (Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0. 1% Triton X-100 permeabilized NIH/3T3 cells labeling Csf1r with AP13911B at 1/25 dilution, followed by Dylight® 488-conjugated goat anti-Rabbit IgG secondary antibody at 1/200 dilution (green). Immunofluorescence image showingCytoplasm staining on NIH/3T3 cell line. Cytoplasmic actin is detected with Dylight® 554 Phalloidin(red). The nuclear counter stain is DAPI (blue).)

Western Blot

(All lanes : Anti-Mouse Csf1r Antibody (C-term) at 1:1000-2000 dilution Lane 1: Mouse heart tissue lysate Lane 2: Mouse skeletal muscle tissue lysate Lane 3: Mouse spleen tissue lysate Lane 4: Rat brain tissue lysate Lysates/proteins at 20 ?g per lane. Secondary Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/10000 dilution. Predicted band size : 109 kDa Blocking/Dilution buffer: 5% NFDM/TBST.)

Western Blot (All lanes : Anti-Mouse Csf1r Antibody (C-term) at 1:1000-2000 dilution Lane 1: Mouse heart tissue lysate Lane 2: Mouse skeletal muscle tissue lysate Lane 3: Mouse spleen tissue lysate Lane 4: Rat brain tissue lysate Lysates/proteins at 20 ?g per lane. Secondary Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/10000 dilution. Predicted band size : 109 kDa Blocking/Dilution buffer: 5% NFDM/TBST.)

Western Blot

(Mouse Csf1r Antibody (C-term) western blot analysis in mouse heart tissue lysates (35ug/lane).This demonstrates the Csf1r antibody detected the Csf1r protein (arrow).)

Western Blot (Mouse Csf1r Antibody (C-term) western blot analysis in mouse heart tissue lysates (35ug/lane).This demonstrates the Csf1r antibody detected the Csf1r protein (arrow).)

Western Blot

(All lanes : Anti-Mouse Csf1r Antibody (C-term) at 1:1000 dilution Lane 1: Mouse heart lysate Lane 2: Mouse skeletal muscle lysate Lane 3: Rat skeletal muscle lysate Lysates/proteins at 20 ?g per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 109 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.)

Western Blot (All lanes : Anti-Mouse Csf1r Antibody (C-term) at 1:1000 dilution Lane 1: Mouse heart lysate Lane 2: Mouse skeletal muscle lysate Lane 3: Rat skeletal muscle lysate Lysates/proteins at 20 ?g per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 109 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.)

Immunohistochemistry

(AP13911b staining Mouse Csf1r in mouse lung tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0.5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.)

Immunohistochemistry (AP13911b staining Mouse Csf1r in mouse lung tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0.5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.)
Related Product Information for anti-Csf1r antibody
Csf1r is a protein tyrosine-kinase transmembrane receptor for CSF1 and IL34.
Product Categories/Family for anti-Csf1r antibody
References
Gueller, S., et al. J. Leukoc. Biol. 88(4):699-706(2010)
Nagamachi, A., et al. Dev. Biol. 345(2):226-236(2010)
Wei, S., et al. J. Leukoc. Biol. 88(3):495-505(2010)
Maitra, R., et al. J. Immunol. 185(3):1485-1491(2010)
Aikawa, Y., et al. Nat. Med. 16(5):580-585(2010)

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
macrophage colony-stimulating factor 1 receptor
NCBI Official Synonym Full Names
colony stimulating factor 1 receptor
NCBI Official Symbol
Csf1r
NCBI Official Synonym Symbols
Fms; CD115; Csfmr; Fim-2; CSF-1R; M-CSFR; M-CSF-R; AI323359
NCBI Protein Information
macrophage colony-stimulating factor 1 receptor
UniProt Protein Name
Macrophage colony-stimulating factor 1 receptor
UniProt Gene Name
Csf1r
UniProt Synonym Gene Names
Csfmr; Fms; CSF-1-R; CSF-1R; M-CSF-R
UniProt Entry Name
CSF1R_MOUSE

Uniprot Description

CSFR: an oncogenic tyrosine kinase receptor for CSF-1 (M-CSF). Drives growth and development of monocytes. Binding of CSF-1 induces receptor dimerization, activation and autophosphorylation of cytoplasmic tyrosine residues used as docking sites for SH2-containing signaling proteins. There are at least five major tyrosine autophosphorylation sites. Two point mutations seen in 10-20% of patients with acute myeloid leukemia, chronic myelomonocytic leukemia or myelodysplasia. One mutation appears to be both somatic and germline, and disrupts Cbl binding and receptor turnover. v-fms lacks the Cbl binding site and causes feline leukemia. Mutations may also develop after chemotherapy for lymphoma. A distinct point mutation was found in some cases of hepatocellular carcinoma and related to increased expression, and another mutation was found in 2 of 40 patients with idiopathic myelofibrosis. Expression is elevated in breast tumors and cell lines, and expression in xenografts and transgenic mice has been correlated with xenograft growth and breast cancer development. Inhibitors: Ki-20227 and other Kit/PDGFR inhibitors.

Protein type: EC 2.7.10.1; Kinase, protein; Protein kinase, TK; Oncoprotein; Membrane protein, integral; Protein kinase, tyrosine (receptor); TK group; PDGFR family

Cellular Component: cell surface; membrane; integral to membrane; plasma membrane; intracellular; receptor complex

Molecular Function: macrophage colony stimulating factor receptor activity; protein homodimerization activity; nucleotide binding; transmembrane receptor protein tyrosine kinase activity; protein phosphatase binding; protein kinase activity; transferase activity; protein binding; cytokine binding; protein-tyrosine kinase activity; transferase activity, transferring phosphorus-containing groups; kinase activity; ATP binding

Biological Process: regulation of bone resorption; phosphoinositide-mediated signaling; peptidyl-tyrosine phosphorylation; immune system process; positive regulation of osteoclast differentiation; protein amino acid autophosphorylation; cytokine and chemokine mediated signaling pathway; osteoclast differentiation; protein amino acid phosphorylation; positive regulation of tyrosine phosphorylation of Stat3 protein; cell proliferation; regulation of cell shape; positive regulation of cell proliferation; ruffle organization and biogenesis; innate immune response; hemopoiesis; phosphatidylinositol metabolic process; positive regulation of protein amino acid phosphorylation; inflammatory response; intercellular junction maintenance; phosphorylation; transmembrane receptor protein tyrosine kinase signaling pathway; positive regulation of cell migration

Research Articles on Csf1r

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Product Notes

The Csf1r csf1r (Catalog #AAA9207852) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 895-923. The Mouse Csf1r Antibody (C-term) reacts with Mouse (Predicted Reactivity: Rat) and may cross-react with other species as described in the data sheet. AAA Biotech's Csf1r can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA), Immunohistochemistry (IHC), Immunofluorescence (IF). WB~~1:1000 IF~~1:25 IHC-P~~1:25. Researchers should empirically determine the suitability of the Csf1r csf1r for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Csf1r, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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