BCIP/NBT chromogenic kit Western Blot Related Reagent
BCIP/NBT chromogenic kit
Synonyms
BCIP/NBT chromogenic kit; BCIP/NBT chromogenic kit western blot related reagent
Assay Procedure
1. After the peroxidase detection, wash fully with TBS 4 times and each time for 5 minutes. Do not use phosphate buffer. 2. Add one drop of chromogenic reagent A and B respectively into 1ml of distilled water and mix evenly. Add it to the sample and colorate in dark for 10-30 minutes. If no background appearing, keep coloration until satisfied. 3. Wash fully with distilled water to stop reaction. Counterstain with nuclear fast red if necessary. 4. Seal sllide with water soluble sealing reagent (MyBioSource can provide). Observe with microscope.
Preparation and Storage
Store at -20 degree C for one year.
Related Product Information for BCIP/NBT chromogenic kit western blot related reagent
Alkaline Phosphatase is one of the important makers of immunology and molecular biology. The insoluble chromogenic substrate mainly contains naphthol phosphate and BCIP. BCIP colors fresh blue working with NBT, and applies to immunohistochemistry and various dot detection
Product Categories/Family for BCIP/NBT chromogenic kit western blot related reagent
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Product Notes
The BCIP/NBT chromogenic kit (Catalog #AAA176752) is a Western Blot Related Reagent and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "BCIP/NBT chromogenic kit, Western Blot Related Reagent" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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