Western Blot (WB)
(Anti-Alpha Amylase 1 antibody, MBS175466, Western blottingLane 1: Rat Liver Tissue LysateLane 2: Rat Intestine Tissue Lysate )
Rabbit Alpha Amylase 1 Polyclonal Antibody | anti-AMY1A antibody
Anti-Alpha Amylase 1 antibody
Gene Names
AMY1B; AMY1
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry
Purity
Immunogen affinity purified.
Synonyms
Alpha Amylase 1; Polyclonal Antibody; Anti-Alpha Amylase 1 antibody; Alpha-amylase 1; amylase; alpha 1A(salivary); 1 antibody; 4-alpha-D-glucan glucanohydrolase 1 antibody; Alpha-amylase 1 antibody; AMY1_HUMAN antibody; AMY1A antibody; AMY1B antibody; AMY1C antibody; Salivary alpha-amylase antibody; anti-AMY1A antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Immunogen affinity purified.
Form/Format
Lyophilized
Sequence Length
511
Applicable Applications for anti-AMY1A antibody
Western Blot (WB), Immunohistochemistry (IHC) Paraffin
Application Notes
WB: 0.1-0.5ug/ml; Tested Species: Human, Mouse, Rat
IHC-P: 0.5-1ug/ml; Tested Species: Rat; Predicted Species: Rat; Antigen Retrieval: By Heat
Tested Species: In-house tested species with positive results.
Predicted Species: Species predicted to be fit for product based on sequence similarties.
By Heat: Boiling paraffin section in 10mM citrate buffer, pH6, for 20 mins is required for staining of formalin/paraffin sections.
Other application have not been tested.
Optimal dilutions should be determined by end user.
IHC-P: 0.5-1ug/ml; Tested Species: Rat; Predicted Species: Rat; Antigen Retrieval: By Heat
Tested Species: In-house tested species with positive results.
Predicted Species: Species predicted to be fit for product based on sequence similarties.
By Heat: Boiling paraffin section in 10mM citrate buffer, pH6, for 20 mins is required for staining of formalin/paraffin sections.
Other application have not been tested.
Optimal dilutions should be determined by end user.
Immunogen
A synthetic peptide corresponding to a sequence at the N-terminus of human Alpha Amylase 1 (30-44aa HLFEWRWVDIALECE), different from the related rat and mouse sequences by one amino acid.
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Preparation and Storage
At -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.
Western Blot (WB)
(Anti-Alpha Amylase 1 antibody, MBS175466, Western blottingLane 1: Rat Liver Tissue LysateLane 2: Rat Intestine Tissue Lysate )
Western Blot (WB)
(Anti-Alpha Amylase 1 antibody, MBS175466, Western blottingLane 1: Rat Liver Tissue LysateLane 2: Rat Intestine Tissue Lysate )
Immunohistochemistry (IHC)
(Anti-Alpha Amylase 1 antibody, MBS175466, IHC(P)IHC(P): Rat Spleen Tissue )
Immunohistochemistry (IHC)
(Anti-Alpha Amylase 1 antibody, MBS175466, IHC(P)IHC(P): Rat Spleen Tissue )
Related Product Information for anti-AMY1A antibody
Description: Rabbit IgG polyclonal antibody for Alpha-amylase 1(AMY1A) detection. Tested with WB, IHC-P in Human, Mouse, Rat.
Background: Amylase is an enzyme that catalyses the breakdown of starch into sugars. Amylase is present in human saliva, where it begins the chemical process of digestion. By in situ hybridization combined with high resolution cytogenetics, the amylase gene is mapped to 1p21.Amylase enzymes find use in bread making and to break down complex sugars such as starch(found in flour) into simple sugars. Yeast then feeds on these simple sugars and converts it into the waste products of alcohol and CO2.
Background: Amylase is an enzyme that catalyses the breakdown of starch into sugars. Amylase is present in human saliva, where it begins the chemical process of digestion. By in situ hybridization combined with high resolution cytogenetics, the amylase gene is mapped to 1p21.Amylase enzymes find use in bread making and to break down complex sugars such as starch(found in flour) into simple sugars. Yeast then feeds on these simple sugars and converts it into the waste products of alcohol and CO2.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Official Full Name
Alpha-amylase 1
NCBI Official Synonym Full Names
amylase, alpha 1B (salivary)
NCBI Official Symbol
AMY1B
NCBI Official Synonym Symbols
AMY1
NCBI Protein Information
alpha-amylase 1; glycogenase; salivary alpha-amylase; salivary amylase alpha 1B; amylase, salivary, alpha-1B; 1,4-alpha-D-glucan glucanohydrolase 1
UniProt Protein Name
Alpha-amylase 1
UniProt Gene Name
AMY1A
UniProt Synonym Gene Names
AMY1
UniProt Entry Name
AMY1_HUMAN
NCBI Description
Amylases are secreted proteins that hydrolyze 1,4-alpha-glucoside bonds in oligosaccharides and polysaccharides, and thus catalyze the first step in digestion of dietary starch and glycogen. The human genome has a cluster of several amylase genes that are expressed at high levels in either salivary gland or pancreas. This gene encodes an amylase isoenzyme produced by the salivary gland. [provided by RefSeq, Jul 2008]
Uniprot Description
AMY1: Monomer. Belongs to the glycosyl hydrolase 13 family.
Protein type: Hydrolase; Carbohydrate Metabolism - starch and sucrose; Secreted; EC 3.2.1.1; Secreted, signal peptide
Chromosomal Location of Human Ortholog: 1p21
Cellular Component: extracellular space
Molecular Function: alpha-amylase activity; protein binding; metal ion binding
Biological Process: carbohydrate metabolic process; digestion
Research Articles on AMY1A
Similar Products
Product Notes
The AMY1A amy1a (Catalog #AAA175466) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-Alpha Amylase 1 antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Alpha Amylase 1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC) Paraffin. WB: 0.1-0.5ug/ml; Tested Species: Human, Mouse, Rat IHC-P: 0.5-1ug/ml; Tested Species: Rat; Predicted Species: Rat; Antigen Retrieval: By Heat Tested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for product based on sequence similarties. By Heat: Boiling paraffin section in 10mM citrate buffer, pH6, for 20 mins is required for staining of formalin/paraffin sections. Other application have not been tested. Optimal dilutions should be determined by end user. Researchers should empirically determine the suitability of the AMY1A amy1a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Alpha Amylase 1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
