Testing Data
Mouse anti-Human CD3 Monoclonal Antibody | anti-CD3 antibody
anti-human CD3-FITC
Reactivity
Human
Applications
Flow Cytometry, Functional Assay, Western Blot
Synonyms
CD3; Monoclonal Antibody; anti-human CD3-FITC; Monoclonal anti-human CD3/FITC; anti-CD3 antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG1
Clone Number
UCHT1
Form/Format
50 mM Sodium Phosphate pH 7.5, 100 mM Potassium Chloride, 150mM NaCl, 5% Glycerol, 0.2% BSA.
Concentration
Volume: 0.2 ml (varies by lot)
Sequence Length
167
Applicable Applications for anti-CD3 antibody
Flow Cytometry (FC/FACS), Western Blot (WB), PS
Application Notes
1:50 (or use 1.6ul of concentrated stock per 5 x 105-cell test)
Performance
Five x 105 ficoll prepared peripheral blood mononuclear cells were washed and pre incubated 10 minutes with 20ul of 250 ug/ml human IgG (to block non specific binding), after which they were incubated 45 minutes on ice with 80 ul of anti-CD3/FITC at a 1:50 dilution factor (10ug/ml). Cells were washed three times, fixed and analyzed by FACS. A 78% sub population of the cells stained positive with a mean shift of 1.67 log10 fluorescent units when compared to a Mouse IgG1/FITC negative control at a similar concentration. Binding was blocked when cells were pre incubated 10 minutes with 20 ml of 0.5 mg/ml anti-CD3 antibody.
Conjugation
FITC
Production
Protein A purified antibody from tissue culture supernatant was reacted with FITC. Unconjugated FITC was separated from antibody/FITC conjugate by desalting column. The antibody/FITC conjugate is at 0.5 mg/ml with a Fluorescein/IgG molar ratio of 11.6.
Buffer
50 mM Sodium Phosphate pH 7.5, 100 mM Potassium Chloride, 150mM NaCl, 5% Glycerol, 0.2% BSA, 0.04% NaN3 (as a preservative).
Immunogen
Human thymocytes/Sezary T cells
Preparation and Storage
Store at 2 - 5 degree C. Freeze/Thawing is not recommended. Protect from light
Product should retain activity for at least 12 months after shipping date when stored as recommended.
Product should retain activity for at least 12 months after shipping date when stored as recommended.
Testing Data
Testing Data
Related Product Information for anti-CD3 antibody
Information: The human CD3/T cell receptor (TcR) complex is made up of at least five CD3 proteins (gamma, delta, epsilon, h, z) in association with either alpha/beta or gamma/delta proteins of the TcR. The TcR recognizes antigens in association with MHC molecules after which protein chains of the CD3 complex mediate activation signals triggered by TcR antigen binding. CD3 is expressed on greater than 95% of circulating human peripheral T cells. Antibody UCHT1 recognizes the 20 kd episilon chain of the CD3 molecule complex. Antibody UCHT1 will activate T cells expressing CD3epsilon.
References
P.C. L. Beverly & R.E. Callard, Eur J Immunol (1981) 11: 329-334. Leukocyte Typing IV (W. Knapp, et al, eds.) Oxford University Press, Oxford, (1989) p. 290-314. A. Salmeron, et al, (1991) J Immunol 147: 3047-3052. G. Thibault & P. Bardos, (1995) J Immunol 154: 3814-3820.
NCBI and Uniprot Product Information
NCBI GI #
Molecular Weight
18,897 Da
NCBI Official Full Name
cd3
UniProt Protein Name
CD3gamma/delta
Protein Family
UniProt Gene Name
cd3g
UniProt Entry Name
P79951_XENLA
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Product Notes
The CD3 cd3g (Catalog #AAA666119) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The anti-human CD3-FITC reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CD3 can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS), Western Blot (WB), PS. 1:50 (or use 1.6ul of concentrated stock per 5 x 105-cell test). Researchers should empirically determine the suitability of the CD3 cd3g for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD3, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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