VTA1 recombinant protein

VTA1, 1-307aa, Human, His tag, E Coli

Cat. Num. AAA204619

• Gene Names: VTA1; DRG1; LIP5; SBP1; DRG-1; My012; C6orf55; HSPC228
• Applications: SDS-Page
• Purity: > 95% by SDS-PAGE
• Synonyms: VTA1; 1-307aa; Human; His tag; E Coli; vacuolar protein sorting-associated protein VTA1 homolog; DRG-1; DRG1; LIP5; SBP1; VTA1 recombinant protein

• Host: E Coli
• Purity/Purification: > 95% by SDS-PAGE
• Form/Format: Liquid. In 20mM Tris-HCl Buffer (pH 8.0) containing 2mM DTT, 10% glycerol, 200mM NaCl
• Concentration: 1mg/ml (determined by Bradford assay) (varies by lot)
• Sequence: MGSSHHHHHH SSGLVPRGSH MGSHMAALAP LPPLPAQFKS IQHHLRTAQE HDKRDPVVAY YCRLYAMQTG MKIDSKTPEC RKFLSKLMDQ LEALKKQLGD NEAITQEIVG CAHLENYALK MFLYADNEDR AGRFHKNMIK SFYTASLLID VITVFGELTD ENVKHRKYAR WKATYIHNCL KNGETPQAGP VGIEEDNDIE ENEDAGAASL PTQPTQPSSS STYDPSNMPS GNYTGIQIPP GAHAPANTPA EVPHSTGVAS NTIQPTPQTI PAIDPALFNT ISQGDVRLTP EDFARAQKYC KYAGSALQYE DVSTAVQNLQ KALKLLTTGR E
• Sequence Length: 307

• Applicable Applications for VTA1 recombinant protein: SDS-PAGE
• Antigen Species: Human
• Tag Information: His-tag
• Preparation and Storage: Can be stored at 4 degree C short term (1-2 weeks).; For long term storage, aliquot and store at -20 degree C or -70 degree C.; Avoid repeated freezing and thawing cycles.

SDS-Page

Related Product Information for VTA1 recombinant protein

VTA1 (Vps20-associated 1 homolog), also known as LIP5 (LYST-interacting protein 5), DRG1 (Dopamine-responsive protein DRG-1), SBP1 (SKD1 bindingprotein 1), belongs to the VTA1 family. This protein Involved in the endosomal multivesicular bodies (MVB) pathway. MVBs contain intraluminal vesicles (ILVs) that are generated by invagination and scission from the limiting membrane of the endosome and mostly are delivered to lysosomes enabling degradation of membrane proteins, such as stimulated growth factor receptors, lysosomal enzymes and lipids. Also, VTA1 is known to interact with CHMP1B, CHMP2A, CHMP5, VPS4B, KIAA0174 (IST1) and possibly CHMP3. Recombinant human VTA1 protein, fused to His-tag at N-terminus, was expressed in E Coli and purified by using conventional chromatography techniques.

Product Categories/Family for VTA1 recombinant protein

Transportation

References

Ward D.M., et al. (2005) J. Biol. Chem. 280:10548-10555; Shim S., et al. (2008) Mol. Biol. Cell 19:2661-2672

NCBI and Uniprot Product Information

• NCBI GI #: 21361741
• NCBI GeneID: 51534
• NCBI Accession #: NP_057569
• NCBI GenBank Nucleotide #: NM_016485.4
• UniProt Accession #: Q9NP79; Q5TGM1; Q6IAE8; Q9H0R2; Q9H3K9; Q9P0Q0; B4DW55; E1P594; E7ETQ7
• Molecular Weight: 36.5kDa (331aa), confirmed by MALDI-TOF
• NCBI Official Full Name: vacuolar protein sorting-associated protein VTA1 homolog isoform a
• NCBI Official Synonym Full Names: vesicle trafficking 1
• NCBI Official Symbol: VTA1
• NCBI Official Synonym Symbols: DRG1; LIP5; SBP1; DRG-1; My012; C6orf55; HSPC228
• NCBI Protein Information: vacuolar protein sorting-associated protein VTA1 homolog
• UniProt Protein Name: Vacuolar protein sorting-associated protein VTA1 homolog
• Protein Family: Vacuolar protein sorting-associated protein
• UniProt Gene Name: VTA1
• UniProt Synonym Gene Names: C6orf55; DRG-1; LIP5; SBP1

NCBI Description

C6ORF55 encodes a protein involved in trafficking of the multivesicular body, an endosomal compartment involved in sorting membrane proteins for degradation in lysosomes (Ward et al., 2005 [PubMed 15644320]).[supplied by OMIM, Mar 2008]

Uniprot Description

Involved in the endosomal multivesicular bodies (MVB) pathway. MVBs contain intraluminal vesicles (ILVs) that are generated by invagination and scission from the limiting membrane of the endosome and mostly are delivered to lysosomes enabling degradation of membrane proteins, such as stimulated growth factor receptors, lysosomal enzymes and lipids. Thought to be a cofactor of VPS4A/B, which catalyzes disassembles membrane-associated ESCRT-III assemblies. Involved in the sorting and down-regulation of EGFR (). Involved in HIV-1 budding.