Western Blot (WB)
(Western blot analysis of extracts from Jurkat, using Ankyrin erythroid Antibody. The lane on the left was treated with blocking peptide.)
Rabbit Ankyrin erythroid Polyclonal Antibody | anti-ANK1 antibody
Ankyrin erythroid Antibody
Predicted Reactivity: Pig(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%)
Predicted Reactivity: Pig(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%)
ELISA(peptide): 1:20,000-1:40,000
Western Blot (WB)
(Western blot analysis of extracts from Jurkat, using Ankyrin erythroid Antibody. The lane on the left was treated with blocking peptide.)
Western Blot (WB)
(Western blot analysis of extracts from Jurkat, using Ankyrin erythroid Antibody. The lane on the left was treated with blocking peptide.)
Post Translational Modifications: Regulated by phosphorylation.Palmitoylated.Hydroxylated by HIF1AN at several asparagine and 1 aspartate residue within ANK repeat region. Hydroxylation seems to increase the conformational stability of this region and may also modulate protein-protein interactions mediated by the ANK repeat region.
Subcellular Location: Cytoplasm>Cytoskeleton. Note: Probably the other erythrocyte (Er) isoforms, are located near the surface of erythrocytic plasma membrane.Membrane. Cytoplasm>Myofibril>Sarcomere>M line. Note: Colocalizes with OBSCN isoform 3/obscurin at the M line in differentiated skeletal muscle cells.Sarcoplasmic reticulum. Sarcoplasmic reticulum. Sarcoplasmic reticulum.
Tissue Specificity: Isoform Mu17, isoform Mu18, isoform Mu19 and isoform Mu20 are expressed in skeletal muscle. Isoform Br21 is expressed in brain.
Subunit Structure: Interacts with a number of integral membrane proteins and cytoskeletal proteins. Interacts (via N-terminus) with SPTB/spectrin (beta chain). Interacts (via N-terminus ANK repeats) with SLC4A1/erythrocyte membrane protein band 3 (via cytoplasmic N-terminus). Also interacts with TTN/titin. Isoform Mu17 interacts with OBSCN isoform 3/obscurin. Interacts with HIF1AN.
Similarity: The 55 kDa regulatory domain is involved in regulating binding of SPTB/spectrin (beta chain) and SLC4A1/erythrocyte membrane protein band 3.The ANK repeat region forms a spiral around a large central cavity and is involved in binding of ion transporters.The tandem configuration of the two ZU5 and the UPA domains forms a structural supramodule termed ZZU. ZU5-1 mediates interaction with beta-spectrin, and the ZU5-1/UPA interface is required for ankyrin's function other than binding to spectrin (By similarity).
NCBI and Uniprot Product Information
Predicted Molecular Weight: (Calculated)206kDa.
NCBI Description
Ankyrins are a family of proteins that link the integral membrane proteins to the underlying spectrin-actin cytoskeleton and play key roles in activities such as cell motility, activation, proliferation, contact and the maintenance of specialized membrane domains. Multiple isoforms of ankyrin with different affinities for various target proteins are expressed in a tissue-specific, developmentally regulated manner. Most ankyrins are typically composed of three structural domains: an amino-terminal domain containing multiple ankyrin repeats; a central region with a highly conserved spectrin binding domain; and a carboxy-terminal regulatory domain which is the least conserved and subject to variation. Ankyrin 1, the prototype of this family, was first discovered in the erythrocytes, but since has also been found in brain and muscles. Mutations in erythrocytic ankyrin 1 have been associated in approximately half of all patients with hereditary spherocytosis. Complex patterns of alternative splicing in the regulatory domain, giving rise to different isoforms of ankyrin 1 have been described. Truncated muscle-specific isoforms of ankyrin 1 resulting from usage of an alternate promoter have also been identified. [provided by RefSeq, Dec 2008]
Uniprot Description
ANK1: Attaches integral membrane proteins to cytoskeletal elements; binds to the erythrocyte membrane protein band 4.2, to Na-K ATPase, to the lymphocyte membrane protein GP85, and to the cytoskeletal proteins fodrin, tubulin, vimentin and desmin. Erythrocyte ankyrins also link spectrin (beta chain) to the cytoplasmic domain of the erythrocytes anion exchange protein; they retain most or all of these binding functions. Defects in ANK1 are a cause of spherocytosis type 1 (SPH1); also called hereditary spherocytosis type 1 (HS1). Spherocytosis is a hematologic disorder leading to chronic hemolytic anemia and characterized by numerous abnormally shaped erythrocytes which are generally spheroidal. Inheritance can be autosomal dominant or recessive. 21 isoforms of the human protein are produced by alternative promoter.
Protein type: Adaptor/scaffold; Cell development/differentiation; Endoplasmic reticulum; Motility/polarity/chemotaxis
Chromosomal Location of Human Ortholog: 8p11.1
Cellular Component: cortical cytoskeleton; postsynaptic membrane; cytoskeleton; sarcoplasmic reticulum; basolateral plasma membrane; plasma membrane; M band; axolemma; Z disc; cytosol; nucleus; sarcolemma
Molecular Function: protein binding; enzyme binding; spectrin binding; structural constituent of cytoskeleton; cytoskeletal adaptor activity; structural molecule activity; ATPase binding
Biological Process: axon guidance; iron ion homeostasis; erythrocyte development; ER to Golgi vesicle-mediated transport; exocytosis; monovalent inorganic cation transport; cytoskeleton organization and biogenesis; signal transduction; porphyrin biosynthetic process; maintenance of epithelial cell polarity
Disease: Spherocytosis, Type 1
Research Articles on ANK1
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Product Notes
The ANK1 ank1 (Catalog #AAA9612158) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Ankyrin erythroid Antibody reacts with Human, Mouse, Rat Predicted Reactivity: Pig(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%) and may cross-react with other species as described in the data sheet. AAA Biotech's Ankyrin erythroid can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA). WB: 1:500-1:2000 ELISA(peptide): 1:20,000-1:40,000. Researchers should empirically determine the suitability of the ANK1 ank1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Ankyrin erythroid, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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