Western Blot (WB)
(The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human VTA1 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: HepG2 cell lysate Lane 2.: 293T cell lysate Lane 3.: HeLa cell lysate Lane 4.: MCF7 cell lysate Lane 5.: A549 cell lysate Lane 6.: Jurkat cell lysate Lane 7.: K562 cell lysate Lane 8.: LnCaP cell lysate Lane 9.: U937 cell lysate Lane 10.: A431 cell lysate )
Mouse anti-Human VTA1 Monoclonal Antibody | anti-VTA1 antibody
VTA1 antibody
For long term storage, aliquot and store at -20 degree C.
Avoid repeated freezing and thawing cycles.
Western Blot (WB)
(The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human VTA1 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: HepG2 cell lysate Lane 2.: 293T cell lysate Lane 3.: HeLa cell lysate Lane 4.: MCF7 cell lysate Lane 5.: A549 cell lysate Lane 6.: Jurkat cell lysate Lane 7.: K562 cell lysate Lane 8.: LnCaP cell lysate Lane 9.: U937 cell lysate Lane 10.: A431 cell lysate )
Western Blot (WB)
(The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human VTA1 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: HepG2 cell lysate Lane 2.: 293T cell lysate Lane 3.: HeLa cell lysate Lane 4.: MCF7 cell lysate Lane 5.: A549 cell lysate Lane 6.: Jurkat cell lysate Lane 7.: K562 cell lysate Lane 8.: LnCaP cell lysate Lane 9.: U937 cell lysate Lane 10.: A431 cell lysate )
Immunofluorescence (IF)
(ICC/IF analysis of VTA1 in Hep3B cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human VTA1 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).)
Immunofluorescence (IF)
(ICC/IF analysis of VTA1 in Hep3B cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human VTA1 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).)
NCBI and Uniprot Product Information
NCBI Description
C6ORF55 encodes a protein involved in trafficking of the multivesicular body, an endosomal compartment involved in sorting membrane proteins for degradation in lysosomes (Ward et al., 2005 [PubMed 15644320]).[supplied by OMIM, Mar 2008]
Uniprot Description
Involved in the endosomal multivesicular bodies (MVB) pathway. MVBs contain intraluminal vesicles (ILVs) that are generated by invagination and scission from the limiting membrane of the endosome and mostly are delivered to lysosomes enabling degradation of membrane proteins, such as stimulated growth factor receptors, lysosomal enzymes and lipids. Thought to be a cofactor of VPS4A/B, which catalyzes disassembles membrane-associated ESCRT-III assemblies. Involved in the sorting and down-regulation of EGFR (). Involved in HIV-1 budding.
Research Articles on VTA1
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Product Notes
The VTA1 vta1 (Catalog #AAA200402) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The VTA1 antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's VTA1 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Flow Cytometry (FC/FACS), Immunocytochemistry (ICC), Immunofluorescence (IF). Researchers should empirically determine the suitability of the VTA1 vta1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "VTA1, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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