Human Voltage Dependent Anion Channel Protein 1 (VDAC1) ELISA Kit | VDAC1 elisa kit

Human Voltage Dependent Anion Channel Protein 1 (VDAC1) ELISA Kit

Cat. Num. AAA2706252

• Gene Names: VDAC1; 5076; ARABIDOPSIS THALIANA VOLTAGE DEPENDENT ANION CHANNEL 1; ATVDAC1; T22N4.9; T22N4_9; voltage dependent anion channel 1
• Reactivity: Human
• Synonyms: Voltage Dependent Anion Channel Protein 1 (VDAC1); Human Voltage Dependent Anion Channel Protein 1 (VDAC1) ELISA Kit; PORIN-31-HL; Porin 31HM; Plasmalemmal porin; Outer mitochondrial membrane protein porin 1; Voltage-dependent anion-selective channel protein 1; VDAC1 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Voltage Dependent Anion Channel Protein 1 (VDAC1).; No significant cross-reactivity or interference between Voltage Dependent Anion Channel Protein 1 (VDAC1) and analogues was observed.
• Sequence Length: 276

• Assay Type: Double-antibody Sandwich
• Samples: Tissue Homogenates, Cell Lysates and Other Biological Fluids
• Detection Range: 0.312-20ng/mL
• Sensitivity: < 0.131ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Voltage Dependent Anion Channel Protein 1 (VDAC1) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Voltage Dependent Anion Channel Protein 1 (VDAC1) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for VDAC1 elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Voltage Dependent Anion Channel Protein 1 (VDAC1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Voltage Dependent Anion Channel Protein 1 (VDAC1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Voltage Dependent Anion Channel Protein 1 (VDAC1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Voltage Dependent Anion Channel Protein 1 (VDAC1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 15232074
• NCBI GeneID: 820914
• NCBI Accession #: NP_186777.1
• NCBI GenBank Nucleotide #: NM_110994.4
• UniProt Accession #: Q9SRH5
• Molecular Weight: 29,425 Da
• NCBI Official Full Name: voltage dependent anion channel 1
• NCBI Official Symbol: VDAC1
• NCBI Official Synonym Symbols: 5076; ARABIDOPSIS THALIANA VOLTAGE DEPENDENT ANION CHANNEL 1; ATVDAC1; T22N4.9; T22N4_9; voltage dependent anion channel 1
• NCBI Protein Information: voltage dependent anion channel 1
• UniProt Protein Name: Mitochondrial outer membrane protein porin 1
• Protein Family: Voltage-dependent anion-selective channel protein
• UniProt Gene Name: VDAC1
• UniProt Synonym Gene Names: AtVDAC1; VDAC-1

NCBI Description

Encodes a voltage-dependent anion channel (VDAC: AT3G01280/VDAC1, AT5G67500/VDAC2, AT5G15090/VDAC3, AT5G57490/VDAC4, AT5G15090/VDAC5). VDACs are reported to be porin-type, beta-barrel diffusion pores. They are prominently localized in the outer mitochondrial membrane and are involved in metabolite exchange between the organelle and the cytosol.

Uniprot Description

Forms a channel through the mitochondrial outer membrane that allows diffusion of small hydrophilic molecules. The channel adopts an open conformation at low or zero membrane potential and a closed conformation at potentials above 30-40 mV. The open state has a weak anion selectivity whereas the closed state is cation-selective (). Involved in plant development at reproductive stage, is important for pollen development and may regulate hydrogen peroxide generation during disease resistance.