vspI restriction enzyme

VspI

Cat. Num. AAA638431

• Purity: Molecular Biology Grade
• Synonyms: VspI; vspI restriction enzyme

• Host: Vibrio species
• Purity/Purification: Molecular Biology Grade

• Dilution Buffer: 10mM Tris-HCl (pH 7.4 at 25 degree C), 100mM KCl, 1mM EDTA, 1mM DTT, 0.2mg/ml BSA and 50% glycerol.
• Digestion of Agarose-embedded DNA: A minimum of 5 units of enzyme is required for digestion of 1ug of agarose-embedded lambda DNA in 16 hours.
• Compatible Ends: Csp6I, MaeI, NdeI, Tru1I
• Supplied With: Provided to simply buffer selection.; R1625: Restriction Enzyme Buffer A, 10X.; R1625-03: Restriction Enzyme Buffer D, 10X.
• Labeled Oligonucleotide (LO) Assay: No detectable degradation of a single-stranded and double-stranded labeled oligonucleotide was observed after incubation with 10 units of restriction endonuclease for 4 hours.
• Ligation/Recutting Assay: After 50-fold overdigestion (3u/ug DNA x 17 hours) with VspI, more than 95% of the DNA fragments can be ligated at a 5'-termini concentration of 0.2uM. More than 95% of these can be recut.
• Number of Recognition Sites in DNA: Lambda: 17; PhiX174: 2; M13mp18/19: 7; pBR322: 1; pUC18/19: 3; pUC57: 3; pTZ19R/U: 3; pBluescriptIIKS(-/+): 3; pBluescriptIISK(-/+): 3; pACYC177: 2; pACYC184: 1
• Overdigestion Assay: No detectable change in the specific fragmentation pattern is observed after 320-fold overdigestion (20u/ug lambda DNA x 16 hours) with VspI.
• Storage Buffer: 10mM Tris-HCl (pH 7.4 at 25 degree C), 100mM KCl, 1mM DTT, 1mM EDTA, 0.2mg/ml BSA and 50% glycerol.
• Thermal Inactivation: Enzyme is inactivated by incubation at 65 degree C for 20min.
• Unit Definition: One unit is defined as the amount of enzyme required to digest 1ug of lambda DNA in 1 hour at 37 degree C in 50ul of assay buffer.
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage, aliquot and store at -20 degree C. Aliquots are stable for at least 6 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Testing Data

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Related Product Information for vspI restriction enzyme

5'-A T^T A A T-3'

Product Categories/Family for vspI restriction enzyme

Molecular Biology; MB-Enzymes; Restriction

NCBI and Uniprot Product Information

• NCBI GI #: 313678858
• NCBI GeneID: 10014193
• NCBI Accession #: YP_004056598.1
• NCBI GenBank Nucleotide #: NC_014760.1
• NCBI Official Full Name: variable surface lipoprotein VspI
• NCBI Official Symbol: vspI
• NCBI Protein Information: variable surface lipoprotein VspI