Vitamin D Receptor/VDR Recombinant Protein | VDR recombinant protein

Vitamin D Receptor/VDR Protein, Mouse, Recombinant (His Tag)

Cat. Num. AAA8123131

• Gene Names: Vdr; Nr1i1
• Purity: >80% as determined by SDS-PAGE
• Synonyms: Vitamin D Receptor/VDR; Vitamin D Receptor/VDR Protein; Mouse; Recombinant (His Tag); Mouse VDR/NR1I1 Protein (His Tag); Nr1i1 Protein; vitamin D (1; 25- dihydroxyvitamin D3) receptor; VDR recombinant protein

• Host: Baculovirus-Insect Cells
• Purity/Purification: >80% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile 20mM Tris, 500mM NaCl, pH8.0, 10% glycerol. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Met1-Ser422

• Species: Mouse
• Endotoxin: <1.0EU per ug of the protein as determined by the LAL method
• Predicted N Terminal: Met
• Tag: C-His
• Protein Construction: A DNA sequence encoding the mouse VDR (P48281)(Met1-Ser422) was fused with a polyhistidine tag at the C-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for VDR recombinant protein

Background: VDR (vitamin D (1,25- dihydroxyvitamin D3) receptor), also known as NR1I1, belongs to the NR1I family, NR1 subfamily. It is composed of three domains: a modulating N-terminal domain, a DNA-binding domain and a C-terminal ligand-binding domain. Vitamin D receptors (VDRs) are members of the NR1I family, which also includes pregnane X (PXR) and constitutive androstane (CAR) receptors, that form heterodimers with members of the retinoid X receptor family. VDRs repress expression of 1alpha-hydroxylase (the proximal activator of 1,25(OH)2D3) and induce expression of the 1,25(OH)2D3 inactivating enzyme CYP24. Also, it has recently been identified as an additional bile acid receptor alongside FXR and may function to protect gut against the toxic and carcinogenic effects of these endobiotics. VDR is expressed in the intestine, thyroid and kidney and has a vital role in calcium homeostasis. It is the nuclear hormone receptor, also called transcription factor that mediates the action of vitamin D3. Inherited mutations in the VDR gene leads to rickets.

References

Moore DD, et al. (2006) The NR1H and NR1I receptors: constitutive androstane receptor, pregnene X receptor, farnesoid X receptor alpha, farnesoid X receptor beta, liver X receptor alpha, liver X receptor beta, and vitamin D receptor. Pharmacol Rev. 58(4):742-59.Szpirer J, et al. (1991)The Sp1 transcription factor gene (SP1) and the 1,25-dihydroxyvitamin D3 receptor gene (VDR) are colocalized on human chromosome arm 12q and rat chromosome 7. Genomics. 11(1):168-73.Germain P, et al. (2006) Overview of nomenclature of nuclear receptors. Pharmacol Rev. 58(4): 685-704.Adorini L, et al. (2006) Vitamin D receptor agonists, cancer and the immune system: an intricate relationship. Curr Top Med Chem. 6(12):1297-301.Luderer HF, et al. (2010) The vitamin D receptor, the skin and stem cells. J Steroid Biochem Mol Biol. 121(1-2):314-6.

NCBI and Uniprot Product Information

• NCBI GI #: 31543944
• NCBI GeneID: 22337
• NCBI Accession #: NP_033530.2
• NCBI GenBank Nucleotide #: NM_009504.4
• UniProt Accession #: P48281; Q922X0
• Molecular Weight: 47,834 Da
• NCBI Official Full Name: vitamin D3 receptor
• NCBI Official Synonym Full Names: vitamin D (1,25-dihydroxyvitamin D3) receptor
• NCBI Official Symbol: Vdr
• NCBI Official Synonym Symbols: Nr1i1
• NCBI Protein Information: vitamin D3 receptor
• UniProt Protein Name: Vitamin D3 receptor
• Protein Family: Vitamin D3 receptor
• UniProt Gene Name: Vdr
• UniProt Synonym Gene Names: Nr1i1; VDR

Uniprot Description

Nuclear receptor for calcitriol, the active form of vitamin D3 which mediates the action of this vitamin on cells. Enters the nucleus upon vitamin D3 binding where it forms heterodimers with the retinoid X receptor/RXR. The VDR-RXR heterodimers bind to specific response elements on DNA and activate the transcription of vitamin D3-responsive target genes. Recruited to promoters via its interaction with BAZ1B/WSTF which mediates the interaction with acetylated histones, an essential step for VDR-promoter association. Plays a central role in calcium homeostasis.