VDR / NR1I1 recombinant protein

Recombinant Human VDR / NR1I1 Protein (His tag)

Cat. Num. AAA2545599

• Gene Names: VDR; NR1I1; PPP1R163
• Purity: > 88 % as determined by SDS-PAGE
• Synonyms: VDR / NR1I1; Recombinant Human VDR / NR1I1 Protein (His tag); NR1I1; VDR / NR1I1 recombinant protein

• Host: Baculovirus-Insect Cells
• Purity/Purification: > 88 % as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile 50mM Tris, 100mM NaCl, pH 8.0, 10% glycerol
• Sequence Length: 427

• Application Notes: The recombinant human VDR consists of 437 amino acids and migrates as an approximately 50 KDa band in SDS-PAGE in SDS-PAGE under reducing conditions as predicted.
• Predicted N Terminal: Met 1
• Endotoxin: < 1.0 EU per mug of the protein as determined by the LAL method
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -70 degree C

SDS-PAGE

Related Product Information for VDR / NR1I1 recombinant protein

Background: VDR (vitamin D (1, 25- dihydroxyvitamin D3)receptor), also known as NR1I1, belongs to the NR1I family, NR1 subfamily. It is composed of three domains: a modulating N-terminal domain, a DNA-binding domain and a C-terminal ligand-binding domain. Vitamin D receptors (VDRs) are members of the NR1I family, which also includes pregnane X (PXR) and constitutive androstane (CAR) receptors, that form heterodimers with members of the retinoid X receptor family. VDRs repress expression of 1alpha-hydroxylase (the proximal activator of 1, 25 (OH)2D3) and induce expression of the 1, 25 (OH)2D3 inactivating enzyme CYP24. Also, it has recently been identified as an additional bile acid receptor alongside FXR and may function to protect gut against the toxic and carcinogenic effects of these endobiotics. VDR is expressed in the intestine, thyroid and kidney and has a vital role in calcium homeostasis. It is the nuclear hormone receptor, also called transcription factor that mediates the action of vitamin D3. Inherited mutations in the VDR gene leads to rickets.

Description: A DNA sequence encoding the human VDR (P11473) (Met 1-Ser 427) was fused with a polyhistidine tag at the C-terminus.

NCBI and Uniprot Product Information

• NCBI GI #: 4507883
• NCBI GeneID: 7421
• NCBI Accession #: NP_000367.1
• NCBI GenBank Nucleotide #: NM_000376.2
• UniProt Accession #: P11473; Q5PSV3; B2R5Q1; G3V1V9
• Molecular Weight: 53,883 Da
• NCBI Official Full Name: vitamin D3 receptor isoform VDRA
• NCBI Official Synonym Full Names: vitamin D (1,25- dihydroxyvitamin D3) receptor
• NCBI Official Symbol: VDR
• NCBI Official Synonym Symbols: NR1I1; PPP1R163
• NCBI Protein Information: vitamin D3 receptor
• UniProt Protein Name: Vitamin D3 receptor
• UniProt Gene Name: VDR
• UniProt Synonym Gene Names: NR1I1; VDR

NCBI Description

This gene encodes the nuclear hormone receptor for vitamin D3. This receptor also functions as a receptor for the secondary bile acid lithocholic acid. The receptor belongs to the family of trans-acting transcriptional regulatory factors and shows sequence similarity to the steroid and thyroid hormone receptors. Downstream targets of this nuclear hormone receptor are principally involved in mineral metabolism though the receptor regulates a variety of other metabolic pathways, such as those involved in the immune response and cancer. Mutations in this gene are associated with type II vitamin D-resistant rickets. A single nucleotide polymorphism in the initiation codon results in an alternate translation start site three codons downstream. Alternative splicing results in multiple transcript variants encoding different proteins. [provided by RefSeq, Feb 2011]

Uniprot Description

Nuclear hormone receptor. Transcription factor that mediates the action of vitamin D3 by controlling the expression of hormone sensitive genes. Recruited to promoters via its interaction with BAZ1B/WSTF which mediates the interaction with acetylated histones, an essential step for VDR-promoter association. Plays a central role in calcium homeostasis.