VCL / Vinculin Recombinant Protein | VCL recombinant protein

Recombinant Mouse VCL / Vinculin Protein (His tag)

Cat. Num. AAA2546793

• Gene Names: Vcl; AA571387; AI462105; AW545629; 9430097D22
• Purity: > 90 % as determined by SDS-PAGE
• Synonyms: VCL / Vinculin; Recombinant Mouse VCL / Vinculin Protein (His tag); VCL; VCL recombinant protein

• Host: Human Cells
• Purity/Purification: > 90 % as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH 7.4
• Sequence Length: 1066

• Application Notes: The recombinant mouse VCL comprises 1077 amino acids and has a predicted molecular mass of 118.2 kDa. The apparent molecular mass of the protein is approximately 118 kDa in SDS-PAGE under reducing conditions.
• Predicted N Terminal: Met
• Endotoxin: < 1.0 EU per mug of the protein as determined by the LAL method
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -70 degree C

SDS-PAGE

Related Product Information for VCL recombinant protein

Background: Vinculin (VCL) is a cytoskeletal protein that is closely related to both cell-matrix interactions and cell-cell junctions. VCL is a membrane-cytoskeletal protein in focal adhesion plaques that is involved in linkage of integrin adhesion molecules to the actin cytoskeleton. The protein contains an acidic N-terminal domain and a basic C-terminal domain separated by a proline-rich middle segment. This protein has multi-ligand properties and has been found to interact with a number of microfilament associated proteins, such as talin, a-actinin, and paxillin, which reportedly bind to either the head or tail domains of vinculin.

Description: A DNA sequence encoding the mouse VCL (NP_033528.3) (Met1-Gln1066) was expressed with a C-terminal polyhistidine tag.

NCBI and Uniprot Product Information

• NCBI GI #: 31543942
• NCBI GeneID: 22330
• NCBI Accession #: NP_033528.3
• NCBI GenBank Nucleotide #: NM_009502.4
• UniProt Accession #: Q64727; Q8BP32; Q8BS46; Q922C5; Q922D9
• Molecular Weight: 116,717 Da
• NCBI Official Full Name: vinculin
• NCBI Official Synonym Full Names: vinculin
• NCBI Official Symbol: Vcl
• NCBI Official Synonym Symbols: AA571387; AI462105; AW545629; 9430097D22
• NCBI Protein Information: vinculin
• UniProt Protein Name: Vinculin
• UniProt Gene Name: Vcl

Uniprot Description

Vinculin: Actin filament (F-actin)-binding protein involved in cell-matrix adhesion and cell-cell adhesion. Regulates cell- surface E-cadherin expression and potentiates mechanosensing by the E-cadherin complex. May also play important roles in cell morphology and locomotion. Exhibits self-association properties. Interacts with NRAP and SORBS1. Interacts with TLN1. Interacts with SYNM. Interacts with CTNNB1 and this interaction is necessary for its localization to the cell-cell junctions and for its function in regulating cell surface expression of E-cadherin. Metavinculin is muscle-specific. Belongs to the vinculin/alpha-catenin family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Cell adhesion; Cytoskeletal; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 14 A3|14 11.53 cM

Cellular Component: actin cytoskeleton; actin filament; adherens junction; cell-cell adherens junction; costamere; cytoplasm; fascia adherens; focal adhesion; intercellular junction; plasma membrane; podosome; protein complex; sarcolemma; stress fiber; Z disc

Molecular Function: alpha-catenin binding; protein binding; Rho GTPase binding; ubiquitin protein ligase binding

Biological Process: apical junction assembly; axon extension; cell adhesion; lamellipodium biogenesis; morphogenesis of an epithelium; regulation of cell migration