Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Nidogen-2 (NID2) Standard Curve.)
Mouse Nidogen-2 (NID2) ELISA Kit | NID2 elisa kit
Mouse Nidogen-2 (NID2) ELISA Kit
Gene Names
Nid2; Ly111; NID-2; AW547149; nidogen-2; entactin-2
Reactivity
Mouse
Synonyms
Nidogen-2 (NID2); Mouse Nidogen-2 (NID2) ELISA Kit; Nidogen 2; osteonidogen; NID2 elisa kit
Reactivity
Mouse
Specificity
Mouse Nidogen-2 (NID2) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse NID2. No significant cross-reactivity or interference between Mouse NID2 and analogues was observed.
Sequence Length
1403
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Nidogen-2 (NID2) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Nidogen-2 (NID2) Standard Curve.)
Related Product Information for NID2 elisa kit
Description: This Mouse Nidogen-2 (NID2) ELISA Kit employs a two-site sandwich ELISA to quantitate NID2 in samples. An antibody specific for NID2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any NID2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for NID2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of NID2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: NID2 protein, 46% identical to NID1, contains a 30-residue signal peptide, 49 primarily central cys residues, 5 potential N-linked glycosylation sites, 2 tyr residues in a potential O-sulfation sequence, and a YGD rather than an RGD cell adhesion sequence.SDS-PAGE analysis showed that NID2 is expressed as a 200-kD protein, larger than the calculated mass of 148 kD, presumably due to oligosaccharide substitution as indicated by hexosamine analysis. Northern blot analysis revealed ubiquitous expression of a 5.5-kb NID2 transcript that was strongest in heart and placenta, moderate in pancreas, kidney, and skeletal muscle, and weakest in brain. Immunoblot analysis detected expression of NID2 in muscle, heart, placenta, kidney, skin, and testis, with weaker expression in liver and brain.
Background: NID2 protein, 46% identical to NID1, contains a 30-residue signal peptide, 49 primarily central cys residues, 5 potential N-linked glycosylation sites, 2 tyr residues in a potential O-sulfation sequence, and a YGD rather than an RGD cell adhesion sequence.SDS-PAGE analysis showed that NID2 is expressed as a 200-kD protein, larger than the calculated mass of 148 kD, presumably due to oligosaccharide substitution as indicated by hexosamine analysis. Northern blot analysis revealed ubiquitous expression of a 5.5-kb NID2 transcript that was strongest in heart and placenta, moderate in pancreas, kidney, and skeletal muscle, and weakest in brain. Immunoblot analysis detected expression of NID2 in muscle, heart, placenta, kidney, skin, and testis, with weaker expression in liver and brain.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
nidogen-2
NCBI Official Synonym Full Names
nidogen 2
NCBI Official Symbol
Nid2
NCBI Official Synonym Symbols
Ly111; NID-2; AW547149; nidogen-2; entactin-2
NCBI Protein Information
nidogen-2
UniProt Protein Name
Nidogen-2
Protein Family
UniProt Gene Name
Nid2
UniProt Synonym Gene Names
NID-2
UniProt Entry Name
NID2_MOUSE
Uniprot Description
Nidogen-2: a cell adhesion glycoprotein widely distributed in basement membranes. Binds to collagens I and IV, to perlecan and to laminin 1. Does not bind fibulins. It probably has a role in cell-extracellular matrix interactions.
Protein type: Secreted; Motility/polarity/chemotaxis; Secreted, signal peptide; Cell adhesion
Cellular Component: extracellular matrix; proteinaceous extracellular matrix; cell surface; extracellular region; basement membrane
Molecular Function: protein binding; calcium ion binding
Biological Process: cell-matrix adhesion; cell adhesion
Research Articles on NID2
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Product Notes
The Mouse NID2 nid2 (Catalog #AAA9718070) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9718070 ELISA Kit recognizes Mouse NID2. It is sometimes possible for the material contained within the vial of "Nidogen-2 (NID2), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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