Immunohistochemistry (IHC)
(Immunohistochemical analysis of C/EBP gamma staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.)
Rabbit C/EBP gamma Polyclonal Antibody | anti-CEBPG antibody
Anti-C/EBP gamma Antibody
IHC: 1/100 - 1/200
IF/ICC: 1/100 - 1/500
Immunohistochemistry (IHC)
(Immunohistochemical analysis of C/EBP gamma staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of C/EBP gamma staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.)
Immunofluoressence (IF)
(Immunofluorescent analysis of C/EBP gamma staining in Jurkat cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.)
Immunofluoressence (IF)
(Immunofluorescent analysis of C/EBP gamma staining in Jurkat cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.)
Western Blot (WB)
(Western blot analysis of C/EBP gamma expression in mouse lung (A) whole cell lysates.)
Western Blot (WB)
(Western blot analysis of C/EBP gamma expression in mouse lung (A) whole cell lysates.)
NCBI and Uniprot Product Information
NCBI Description
The C/EBP family of transcription factors regulates viral and cellular CCAAT/enhancer element-mediated transcription. C/EBP proteins contain the bZIP region, which is characterized by two motifs in the C-terminal half of the protein: a basic region involved in DNA binding and a leucine zipper motif involved in dimerization. The C/EBP family consist of several related proteins, C/EBP alpha, C/EBP beta, C/EBP gamma, and C/EBP delta, that form homodimers and that form heterodimers with each other. CCAAT/enhancer binding protein gamma may cooperate with Fos to bind PRE-I enhancer elements. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Nov 2011]
Uniprot Description
C/EBP-gamma: Transcription factor that binds to the enhancer element PRE-I (positive regulatory element-I) of the IL-4 gene. Might change the DNA-binding specificity of other transcription factors and recruit them to unusual DNA sites. Belongs to the bZIP family. C/EBP subfamily.
Protein type: DNA-binding; Motility/polarity/chemotaxis; Transcription factor
Chromosomal Location of Human Ortholog: 19q13.11
Cellular Component: nucleoplasm; nucleus
Molecular Function: DNA binding; protein binding; protein heterodimerization activity; sequence-specific DNA binding; transcription factor binding
Biological Process: B cell differentiation; enucleate erythrocyte differentiation; immune response; liver development; mRNA metabolic process; natural killer cell mediated cytotoxicity; negative regulation of transcription factor activity; positive regulation of DNA binding; positive regulation of DNA repair; positive regulation of interferon-gamma biosynthetic process; positive regulation of transcription factor activity; positive regulation of transcription from RNA polymerase II promoter; regulation of transcription from RNA polymerase II promoter; transcription from RNA polymerase II promoter
Research Articles on CEBPG
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Product Notes
The CEBPG cebpg (Catalog #AAA8241686) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-C/EBP gamma Antibody reacts with Human, Mouse, Rat, Bovine, Chicken and may cross-react with other species as described in the data sheet. AAA Biotech's C/EBP gamma can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence/Immunocytochemistry (IF/ICC). WB: 1/500 - 1/1000 IHC: 1/100 - 1/200 IF/ICC: 1/100 - 1/500. Researchers should empirically determine the suitability of the CEBPG cebpg for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "C/EBP gamma, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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