Rabbit anti-Human SYK Polyclonal Antibody | anti-SYK antibody

SYK, Phosphorylated (Tyr525/526) (Tyrosine-protein Kinase SYK, Spleen Tyrosine Kinase) (APC)

Cat. Num. AAA6501780

• Gene Names: SYK; p72-Syk
• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot
• Purity: Purified by Immunoaffinity chromatography.
• Synonyms: SYK; Polyclonal Antibody; Phosphorylated (Tyr525/526) (Tyrosine-protein Kinase SYK; Spleen Tyrosine Kinase) (APC); anti-SYK antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human Syk when phosphorylated at Tyr525 and 526.
• Purity/Purification: Purified by Immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).

• Applicable Applications for anti-SYK antibody: Immunohistochemistry (IHC), FLISA, Western Blot (WB)
• Application Notes: IHC: 1:50-1:100; FLISA: 1:1,000; WB: 1:100-1:500; Applications are based on unconjugated antibody.
• Immunogen: Synthetic phosphopeptide corresponding to amino acid residues surrounding Tyr525/526 of human Syk (KLH).
• Conjugate: APC
• Antigen Modification: Phosphorylated
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Do not freeze APC conjugates. Light sensitive. Aliquots are stable for at least 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of anti-Phospho-Syk Pab in mouse liver tissue lysate (35ug/lane). Phospho-Syk(arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)

Product Categories/Family for anti-SYK antibody

Antibodies; Tyrosine Kinases

References

1. Steinberg, M., et al., Blood 104(3):760-767 (2004). 2. Wang, S., et al., World J. Gastroenterol. 10(12):1815-1818 (2004). 3. Hayes, M.J., et al., Mol. Immunol. 41(4):457-468 (2004). 4. Arndt, P.G., et al., J. Biol. Chem. 279(12):10883-10891 (2004). 5. Ding, Y.B., et al., Zhonghua Yi Xue Za Zhi 84(4):290-293 (2004).

NCBI and Uniprot Product Information

• NCBI GI #: 21361553
• NCBI GeneID: 6850
• NCBI Accession #: NP_003168
• NCBI GenBank Nucleotide #: NM_003177
• UniProt Accession #: P43405
• Molecular Weight: 72kDa
• NCBI Official Full Name: tyrosine-protein kinase SYK isoform Syk(L)
• NCBI Official Synonym Full Names: spleen associated tyrosine kinase
• NCBI Official Symbol: SYK
• NCBI Official Synonym Symbols: p72-Syk
• NCBI Protein Information: tyrosine-protein kinase SYK
• UniProt Protein Name: Tyrosine-protein kinase SYK
• Protein Family: Tyrosine-protein kinase
• UniProt Gene Name: SYK
• UniProt Entry Name: KSYK_HUMAN

NCBI Description

This gene encodes a member of the family of non-receptor type Tyr protein kinases. This protein is widely expressed in hematopoietic cells and is involved in coupling activated immunoreceptors to downstream signaling events that mediate diverse cellular responses, including proliferation, differentiation, and phagocytosis. It is thought to be a modulator of epithelial cell growth and a potential tumour suppressor in human breast carcinomas. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2010]

Uniprot Description

Syk: a cytoplasmic tyrosine kinase of the SYK family containing two SH2 domains. Plays a central role in the B cell receptor (BCR) response. An upstream activator of the PI3K, PLCgamma2, and Rac/cdc42 pathways in the BCR response. Required for the sequential events of Fc gamma IIa receptor-mediated phagocytosis. Expression highest in murine spleen, heart, mammary gland and thymus. Two splice variant isoforms have been described.

Protein type: Protein kinase, TK; Protein kinase, tyrosine (non-receptor); Kinase, protein; EC 2.7.10.2; TK group; Syk family

Chromosomal Location of Human Ortholog: 9q22

Cellular Component: T cell receptor complex; extrinsic to internal side of plasma membrane; protein complex; cytoplasm; plasma membrane; nucleus; cytosol; B cell receptor complex

Molecular Function: integrin binding; protein serine/threonine kinase activity; protein binding; protein-tyrosine kinase activity; non-membrane spanning protein tyrosine kinase activity; receptor signaling protein tyrosine kinase activity; protein kinase binding; ATP binding; protein kinase activity

Biological Process: viral reproduction; transcription factor import into nucleus; positive regulation of interleukin-3 biosynthetic process; positive regulation of granulocyte macrophage colony-stimulating factor biosynthetic process; positive regulation of calcium-mediated signaling; regulation of phagocytosis; protein amino acid phosphorylation; activation of JNK activity; leukocyte adhesion; B cell receptor signaling pathway; regulation of transcription factor activity; positive regulation of bone resorption; beta selection; positive regulation of gamma-delta T cell differentiation; angiogenesis; inflammatory response; integrin-mediated signaling pathway; neutrophil chemotaxis; platelet activation; adaptive immune response; regulation of superoxide release; positive regulation of cell adhesion mediated by integrin; positive regulation of mast cell degranulation; regulation of neutrophil degranulation; leukotriene biosynthetic process; cell proliferation; organ morphogenesis; neutrophil activation during immune response; positive regulation of peptidyl-tyrosine phosphorylation; leukocyte activation during immune response; serotonin secretion by platelet; defense response to bacterium; macrophage activation during immune response; positive regulation of B cell differentiation; blood vessel morphogenesis; innate immune response; positive regulation of alpha-beta T cell proliferation; blood coagulation; lymph vessel development; positive regulation of alpha-beta T cell differentiation; transmembrane receptor protein tyrosine kinase signaling pathway; positive regulation of cytokine secretion