SDS-Page
(Recombinant USP7 protein gel 8% SDS-PAGE gel stained with Coomassie Blue MW: 131.3 kDa
Purity: >80%)
USP7 recombinant protein
Recombinant USP7 protein
Shipping Temp: Dry Ice
SDS-Page
(Recombinant USP7 protein gel 8% SDS-PAGE gel stained with Coomassie Blue MW: 131.3 kDa Purity: >80%)
SDS-Page
(Recombinant USP7 protein gel 8% SDS-PAGE gel stained with Coomassie Blue MW: 131.3 kDa
Purity: >80%)
Testing Data
(DUB-Detector assay for USP7 protein activity 100 nM Fluorescent Substrate was incubated with different concentration of USP7 protein for 1 hour at room temperature. The fluorescent intensity of the reaction was measured with an excitation wavelength of 485 nm and an emission wavelength of 535nm.)
Testing Data
(DUB-Detector assay for USP7 protein activity 100 nM Fluorescent Substrate was incubated with different concentration of USP7 protein for 1 hour at room temperature. The fluorescent intensity of the reaction was measured with an excitation wavelength of 485 nm and an emission wavelength of 535nm.)
Testing Data
(DUB-Detector assay for USP7 protein activity 100 nM Fluorescent Substrate was incubated with different concentration of USP7 protein for 1 hour at room temperature. The fluorescent intensity of the reaction was measured with an excitation wavelength of 485 nm and an emission wavelength of 535nm.)
Testing Data
(DUB-Detector assay for USP7 protein activity 100 nM Fluorescent Substrate was incubated with different concentration of USP7 protein for 1 hour at room temperature. The fluorescent intensity of the reaction was measured with an excitation wavelength of 485 nm and an emission wavelength of 535nm.)
Background: USP7 (Ubiquitin Specific Peptidase 7 (Herpes Virus-Associated)) belongs to the peptidase C19 family, which includes ubiquitinyl hydrolases. USP7 de-ubiquitinates target proteins such as FOXO4, p53/TP53, MDM2, UHRF1, PTEN and DAXX. Together with DAXX, USP7 prevents MDM2 self-ubiquitination and enhances the E3 ligase activity of MDM2 towards p53/TP53, thereby promoting p53/TP53 ubiquitination and proteasomal degradation. USP7 de-ubiquitinates p53/TP53, preventing degradation of p53/TP53, and enhances p53/TP53-dependent transcription regulation, cell growth repression and apoptosis. USP7 also de-ubiquitinates FOXO4 and inhibits FOXO4-induced transcriptional activity. Together with DAXX, USP7 is involved in the de-ubiquitination and translocation of PTEN from the nucleus to the cytoplasm, both processes that are counteracted by PML. USP7 de-ubiquitinates UHRF1, which prevents its degradation and promoting DNA methylation by DNMT1.
NCBI and Uniprot Product Information
Uniprot Description
USP7: an enzyme that removes ubiquitin from its protein substrates. Binds to the herpes virus protein VMW110 that may therefore modulate its substrate specificity or activity to stabilize viral proteins. Regulates MDM2 and has a dynamic role in the p53-MDM2 pathway. Is reported to deubiquitinate and stabilize p53.
Protein type: Ubiquitin-specific protease; Protease; EC 3.4.19.12; Ubiquitin conjugating system
Chromosomal Location of Human Ortholog: 16p13.3
Cellular Component: nuclear body; PML body; cytosol; nucleus
Molecular Function: protein C-terminus binding; protein binding; p53 binding; cysteine-type endopeptidase activity; ubiquitin protein ligase binding; ubiquitin-specific protease activity; transcription factor binding
Biological Process: proteasomal ubiquitin-dependent protein catabolic process; protein deubiquitination; maintenance of DNA methylation; viral reproduction; regulation of transcription factor activity; inhibition of NF-kappaB transcription factor; transcription-coupled nucleotide-excision repair; multicellular organismal development; histone deubiquitination
Research Articles on USP7
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Product Notes
The USP7 usp7 (Catalog #AAA388229) is a Recombinant Protein produced from Baculovirus and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "USP7, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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