Human ubiquitin specific peptidase 13 (isopeptidase T-3) ELISA Kit | USP13 elisa kit

Human Ubiquitin carboxyl-terminal hydrolase 13, USP13 ELISA Kit

Cat. Num. AAA9335287

• Gene Names: USP13; ISOT3; IsoT-3
• Reactivity: Human
• Synonyms: ubiquitin specific peptidase 13 (isopeptidase T-3); Human Ubiquitin carboxyl-terminal hydrolase 13; USP13 ELISA Kit; Human Ubiquitin carboxyl-terminal hydrolase 13 (USP13) ELISA kit; ISOT3; IsoT-3; ubiquitin specific protease 13 (isopeptidase T-3) ; ubiquitin thiolesterase 13; USP13 elisa kit

• Reactivity: Human

• Assay Type: Quantitative Sandwich
• Detection Range: 0.625ng/ml-20ng/ml
• Sensitivity: 0.1ng/ml
• Intra-assay Precision: Intra-assay CV (%) is less than 15%.
• Inter-assay Precision: Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean ×100]. All CV% should be compared by concentration, not compared by OD values.
• Preparation and Storage: Store all reagents at 2-8 degree C

Related Product Information for USP13 elisa kit

Background/Introduction: This Quantitative Sandwich ELISA kit is for lab reagent/research use only, not for drug, household, therapeutic or test applications! This kit is intended to be used for determine the level of USP13 (hereafter termed "analyte") in undiluted original Human serum, plasma or tissue homogenates samples. For other sample types please contact tech support to determine compatibility with this assay. This kit is not suitable for assaying non-biological sources of substances.

NCBI and Uniprot Product Information

• NCBI GI #: 215598688
• NCBI GeneID: 8975
• NCBI Accession #: NP_003931.2
• NCBI GenBank Nucleotide #: NM_003940.2
• UniProt Accession #: Q92995; Q96B25; A8K2S3; B4DYF3; D3DNS2
• Molecular Weight: 97,327 Da
• NCBI Official Full Name: ubiquitin carboxyl-terminal hydrolase 13
• NCBI Official Synonym Full Names: ubiquitin specific peptidase 13 (isopeptidase T-3)
• NCBI Official Symbol: USP13
• NCBI Official Synonym Symbols: ISOT3; IsoT-3
• NCBI Protein Information: ubiquitin carboxyl-terminal hydrolase 13; isopeptidase T-3; ubiquitin thioesterase 13; deubiquitinating enzyme 13; ubiquitin thiolesterase 13; ubiquitin-specific-processing protease 13; ubiquitin specific protease 13 (isopeptidase T-3)
• UniProt Protein Name: Ubiquitin carboxyl-terminal hydrolase 13
• UniProt Gene Name: USP13
• UniProt Synonym Gene Names: ISOT3; ISOT-3
• UniProt Entry Name: UBP13_HUMAN

Uniprot Description

USP13: Deubiquitinase that mediates deubiquitination of target proteins such as BECN1, MITF, SKP2 and USP10 and is involved in various processes such as autophagy and endoplasmic reticulum- associated degradation (ERAD). Component of a regulatory loop that controls autophagy and p53/TP53 levels: mediates deubiquitination of BECN1, a key regulator of autophagy, leading to stabilize the PIK3C3/VPS34-containing complexes. Also deubiquitinates USP10, an essential regulator of p53/TP53 stability. In turn, PIK3C3/VPS34- containing complexes regulate USP13 stability, suggesting the existence of a regulatory system by which PIK3C3/VPS34-containing complexes regulate p53/TP53 protein levels via USP10 and USP13. Recruited by nuclear UFD1 and mediates deubiquitination of SKP2, thereby regulating endoplasmic reticulum-associated degradation (ERAD). Mediates stabilization of SIAH2 independently of deubiquitinase activity: binds ubiquitinated SIAH2 and acts by impairing SIAH2 autoubiquitination. Has a weak deubiquitinase activity in vitro and preferentially cleaves 'Lys-63'-linked polyubiquitin chains. In contrast to USP5, it is not able to mediate unanchored polyubiquitin disassembly. Able to cleave ISG15 in vitro; however, additional experiments are required to confirm such data. Belongs to the peptidase C19 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 3.4.19.12; Protease; Ubiquitin conjugating system; Ubiquitin-specific protease

Chromosomal Location of Human Ortholog: 3q26.2-q26.3

Cellular Component: lysosome

Molecular Function: ubiquitin binding; protein binding; omega peptidase activity; zinc ion binding; ubiquitin protein ligase binding; cysteine-type endopeptidase activity; ubiquitin-specific protease activity

Biological Process: proteasomal ubiquitin-dependent protein catabolic process; cell proliferation; protein stabilization; regulation of transcription, DNA-dependent; melanocyte differentiation; autophagy; regulation of autophagy