Rabbit anti-Human IL2RG Polyclonal Antibody | anti-IL2RG antibody

IL2RG Antibody (N-term)

Cat. Num. AAA9214003

• Gene Names: IL2RG; P64; CIDX; IMD4; CD132; SCIDX; IL-2RG; SCIDX1
• Reactivity: Human
• Applications: Western Blot, ELISA, Flow Cytometry, Functional Assay, Immunofluorescence
• Purity: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Synonyms: IL2RG; Polyclonal Antibody; IL2RG Antibody (N-term); Cytokine receptor common subunit gamma; Interleukin-2 receptor subunit gamma; IL-2 receptor subunit gamma; IL-2R subunit gamma; IL-2RG; gammaC; p64; CD132; anti-IL2RG antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: Rabbit Ig
• Specificity: This IL2RG antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 76-101 amino acids from the N-terminal region of human IL2RG.
• Purity/Purification: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Form/Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Sequence Positions: 76-101
• Sequence Length: 369

• Applicable Applications for anti-IL2RG antibody: Western Blot (WB), ELISA (EIA), Flow Cytometry (FC/FACS), Immunofluoresence (IF)
• Application Notes: WB~~1:1000; IF~~1:25; FC~~1:10~50
• Function: Common subunit for the receptors for a variety of interleukins. Probably in association with IL15RA, involved in the stimulation of neutrophil phagocytosis by IL15
• Cellular Location: Cell membrane; Single-pass type I membrane protein. Cell surface
• Antigen Source: HUMAN
• Preparation and Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Western Blot (WB)

(All lanes : Anti-IL2RG Antibody (N-term) at 1:2000 dilutionLane 1: human spleen lysatesLane 2: Jurkat whole cell lysatesLane 3: Daudi whole cell lysatesLane 4: KG-1 whole cell lysatesLane 5: MOLT-4 whole cell lysatesLane 6: HL-60 whole cell lysates Lysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilutionPredicted band size : 42 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)

Western Blot (WB)

(Western blot analysis of lysate from Jurkat cell line, using IL2RG Antibody (N-term). MBS9214003 was diluted at 1:1000. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysate at 20ug.)

Western Blot (WB)

(Western blot analysis of IL2RG Antibody (N-term) in HL-60 cell line lysates (35ug/lane). IL2RG (arrow) was detected using the purified Pab.)

Flow Cytometry (FC/FACS)

(IL2RG Antibody (N-term) flow cytometric analysis of HL-60 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Immunofluoresence(IF)

(Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized human spleen tissue labeling IL2RG with MBS9214003 at 1/25 dilution, followed by AlexaFluor488-conjugated goat anti-rabbit IgG (1583138) secondary antibody at 1/400 dilution (green). Confocal image showing membrane staining on human spleen tissue. The nuclear counter stain is DAPI (blue).)

Related Product Information for anti-IL2RG antibody

IL2RG is an important signaling component of many interleukin receptors, including those of interleukin -2, -4, -7 and -21, and is thus referred to as the common gamma chain. Mutations in this gene cause X-linked severe combined immunodeficiency (XSCID), as well as X-linked combined immunodeficiency (XCID), a less severe immunodeficiency disorder.

Product Categories/Family for anti-IL2RG antibody

Cancer; Immunology; Signal Transduction

References

Ross, J.A., et al. J. Biol. Chem. 285(6):3582-3591(2010)
Ameratunga, R., et al. N. Z. Med. J. 122(1304):46-53(2009)
Clark, P.A., et al. Hum. Genet. 96(4):427-432(1995)

NCBI and Uniprot Product Information

• NCBI GI #: 4557882
• NCBI GeneID: 3561
• NCBI Accession #: NP_000197.1
• NCBI GenBank Nucleotide #: NM_000206.2
• UniProt Accession #: P31785; Q5FC12
• Molecular Weight: 42287
• NCBI Official Full Name: cytokine receptor common subunit gamma
• NCBI Official Synonym Full Names: interleukin 2 receptor, gamma
• NCBI Official Symbol: IL2RG
• NCBI Official Synonym Symbols: P64; CIDX; IMD4; CD132; SCIDX; IL-2RG; SCIDX1
• NCBI Protein Information: cytokine receptor common subunit gamma
• UniProt Protein Name: Cytokine receptor common subunit gamma
• Protein Family: Cytokine receptor
• UniProt Gene Name: IL2RG
• UniProt Synonym Gene Names: IL-2 receptor subunit gamma; IL-2R subunit gamma; IL-2RG
• UniProt Entry Name: IL2RG_HUMAN

NCBI Description

The protein encoded by this gene is an important signaling component of many interleukin receptors, including those of interleukin -2, -4, -7 and -21, and is thus referred to as the common gamma chain. Mutations in this gene cause X-linked severe combined immunodeficiency (XSCID), as well as X-linked combined immunodeficiency (XCID), a less severe immunodeficiency disorder. [provided by RefSeq, Mar 2010]

Uniprot Description

IL2RG: Common subunit for the receptors for a variety of interleukins. Defects in IL2RG are the cause of severe combined immunodeficiency X-linked T-cell-negative/B-cell-positive/NK-cell- negative (XSCID); also known as agammaglobulinemia Swiss type. A form of severe combined immunodeficiency (SCID), a genetically and clinically heterogeneous group of rare congenital disorders characterized by impairment of both humoral and cell- mediated immunity, leukopenia, and low or absent antibody levels. Patients present in infancy recurrent, persistent infections by opportunistic organisms. The common characteristic of all types of SCID is absence of T-cell-mediated cellular immunity due to a defect in T-cell development. Defects in IL2RG are the cause of X-linked combined immunodeficiency (XCID). XCID is a less severe form of X-linked immunodeficiency with a less severe degree of deficiency in cellular and humoral immunity than that seen in XSCID. Belongs to the type I cytokine receptor family. Type 5 subfamily.

Protein type: Receptor, cytokine; Membrane protein, integral

Chromosomal Location of Human Ortholog: Xq13.1

Cellular Component: membrane; integral to plasma membrane; plasma membrane; external side of plasma membrane

Molecular Function: protein binding; interleukin-4 receptor activity; interleukin-2 receptor activity; interleukin-7 receptor activity; interleukin-7 binding; interleukin-2 binding

Biological Process: viral reproduction; immune response; signal transduction

Disease: Combined Immunodeficiency, X-linked; Severe Combined Immunodeficiency, X-linked