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Western Blot (WB) (Western blot analysis of UMPS expression in Jurkat (A) whole cell lysates.)

Rabbit UMPS Polyclonal Antibody | anti-UMPS antibody

Anti-UMPS Antibody

Gene Names
UMPS; OPRT
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry
Purity
Purified by immunogen affinity chromatography
Synonyms
UMPS; Polyclonal Antibody; Anti-UMPS Antibody; Uridine 5'-monophosphate synthase; UMP synthase; anti-UMPS antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Specificity
Recognizes endogenous levels of UMPS protein.
Purity/Purification
Purified by immunogen affinity chromatography
Form/Format
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Sequence Length
205
Applicable Applications for anti-UMPS antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)
Application Notes
WB: 1/500 - 1/2000
IHC: 1/50 - 1/200
IF/ICC: 1/50 - 1/200
Immunogen
Recombinant full length protein of human UMPS
Preparation and Storage
Shipped at 4 degree C. Upon delivery aliquot and store at -20 degree C for one year. Avoid freeze/thaw cycles.

Western Blot (WB)

(Western blot analysis of UMPS expression in Jurkat (A) whole cell lysates.)

Western Blot (WB) (Western blot analysis of UMPS expression in Jurkat (A) whole cell lysates.)

Immunofluorescence (IF)

(Immunofluorescent analysis of UMPS staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.)

Immunofluorescence (IF) (Immunofluorescent analysis of UMPS staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.)
Related Product Information for anti-UMPS antibody
Rabbit polyclonal antibody to UMPS

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
22,922 Da
NCBI Official Full Name
uridine 5'-monophosphate synthase
NCBI Official Synonym Full Names
uridine monophosphate synthetase
NCBI Official Symbol
UMPS
NCBI Official Synonym Symbols
OPRT
NCBI Protein Information
uridine 5'-monophosphate synthase
UniProt Protein Name
Uridine 5'-monophosphate synthase
UniProt Gene Name
UMPS
UniProt Synonym Gene Names
UMP synthase; OPRT; OPRTase; ODC

NCBI Description

This gene encodes a uridine 5'-monophosphate synthase. The encoded protein is a bifunctional enzyme that catalyzes the final two steps of the de novo pyrimidine biosynthetic pathway. The first reaction is carried out by the N-terminal enzyme orotate phosphoribosyltransferase which converts orotic acid to orotidine-5'-monophosphate. The terminal reaction is carried out by the C-terminal enzyme OMP decarboxylase which converts orotidine-5'-monophosphate to uridine monophosphate. Defects in this gene are the cause of hereditary orotic aciduria. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Mar 2010]

Research Articles on UMPS

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Product Notes

The UMPS umps (Catalog #AAA8292201) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-UMPS Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's UMPS can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC). WB: 1/500 - 1/2000 IHC: 1/50 - 1/200 IF/ICC: 1/50 - 1/200. Researchers should empirically determine the suitability of the UMPS umps for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "UMPS, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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