Immunofluorescence (IF)
(Immunofluorescence staining of Hela cells with MBS7107315 at 1:33, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4 degree C.The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).)
Rabbit anti-Human UMPS Polyclonal Antibody | anti-UMPS antibody
UMPS Antibody
Gene Names
UMPS; OPRT
Reactivity
Human
Applications
ELISA, Immunofluorescence
Purity
>95%, Protein G purified
Synonyms
UMPS; Polyclonal Antibody; UMPS Antibody; Uridine 5'-monophosphate synthase; UMP synthase; Orotate phosphoribosyltransferase; OPRT; OPRTaseOrotidine 5'-phosphate decarboxylase; ODC; OMPdecase; OK/SW-cl.21; anti-UMPS antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
>95%, Protein G purified
Form/Format
Liquid
Sequence Length
480
Applicable Applications for anti-UMPS antibody
ELISA (EIA), Immunofluorescence (IF)
Application Notes
IF: 1:50-1:200
Immunogen
Recombinant Human Uridine 5'-monophosphate synthase protein (314-444AA)
Conjugation
Non-conjugated
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Preparation and Storage
Upon receipt, store at -20 degree C or -80 degree C. Avoid repeated freeze.
Immunofluorescence (IF)
(Immunofluorescence staining of Hela cells with MBS7107315 at 1:33, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4 degree C.The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).)
Immunofluorescence (IF)
(Immunofluorescence staining of Hela cells with MBS7107315 at 1:33, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4 degree C.The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).)
Related Product Information for anti-UMPS antibody
cytoplasm, cytosol, nucleus, orotate phosphoribosyltransferase activity, orotidine-5'-phosphate decarboxylase activity, pyrimidine nucleoside biosynthetic process, UMP biosynthetic process
Product Categories/Family for anti-UMPS antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
22,922 Da
NCBI Official Full Name
uridine 5'-monophosphate synthase
NCBI Official Synonym Full Names
uridine monophosphate synthetase
NCBI Official Symbol
UMPS
NCBI Official Synonym Symbols
OPRT
NCBI Protein Information
uridine 5'-monophosphate synthase
UniProt Protein Name
Uridine 5'-monophosphate synthase
Protein Family
UniProt Gene Name
UMPS
UniProt Synonym Gene Names
UMP synthase; OPRT; OPRTase; ODC
NCBI Description
This gene encodes a uridine 5'-monophosphate synthase. The encoded protein is a bifunctional enzyme that catalyzes the final two steps of the de novo pyrimidine biosynthetic pathway. The first reaction is carried out by the N-terminal enzyme orotate phosphoribosyltransferase which converts orotic acid to orotidine-5'-monophosphate. The terminal reaction is carried out by the C-terminal enzyme OMP decarboxylase which converts orotidine-5'-monophosphate to uridine monophosphate. Defects in this gene are the cause of hereditary orotic aciduria. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Mar 2010]
Research Articles on UMPS
1. Partial UMPS-deficiency should be included in the differential diagnosis of mild orotic aciduria.
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Product Notes
The UMPS umps (Catalog #AAA7107315) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The UMPS Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's UMPS can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Immunofluorescence (IF). IF: 1:50-1:200. Researchers should empirically determine the suitability of the UMPS umps for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "UMPS, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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