Uncoupling Protein 1, Mitochondrial (UCP1) Recombinant Protein | UCP1 recombinant protein

Recombinant Uncoupling Protein 1, Mitochondrial (UCP1)

Cat. Num. AAA2011735

• Gene Names: Ucp1; Ucp; Slc25a7; AI385626
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: > 95%
• Synonyms: Uncoupling Protein 1; Mitochondrial (UCP1); Recombinant Uncoupling Protein 1; UCP1 recombinant protein

• Host: E Coli
• Purity/Purification: > 95%
• Form/Format: Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.
• Sequence: The target protein is fused with N-terminal His-Tag, its sequence is listed below.; MGHHHHHHSGSEF-PNLMRNVIIN CTELVTYDLM KGALVNNKIL ADDVPCHLLS ALVAGFCTTL LASPVDVVKT RFINSLPGQY PSVPSCAMSM YTKEGPTAFF KGFVASFLRL GSWNVIMFVC FEQLKKEL
• Sequence Length: 307

• Applicable Applications for UCP1 recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Organism: Mus musculus (Mouse)
• Expression System: Prokaryotic expression
• Residues: Pro179~Leu296 (Accession # P12242) with N-terminal His-Tag
• Endotoxin Level: <1.0EU per 1ug (determined by the LAL method)
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 6678497
• NCBI GeneID: 22227
• NCBI Accession #: NP_033489.1
• NCBI GenBank Nucleotide #: NM_009463.3
• UniProt Accession #: P12242
• Molecular Weight: 14.5kDa
• NCBI Official Full Name: mitochondrial brown fat uncoupling protein 1
• NCBI Official Synonym Full Names: uncoupling protein 1 (mitochondrial, proton carrier)
• NCBI Official Symbol: Ucp1
• NCBI Official Synonym Symbols: Ucp; Slc25a7; AI385626
• NCBI Protein Information: mitochondrial brown fat uncoupling protein 1; UCP 1; thermogenin; solute carrier family 25 member 7; uncoupling protein, mitochondrial; uncoupling protein 1, mitochondrial
• UniProt Protein Name: Mitochondrial brown fat uncoupling protein 1
• Protein Family: Mitochondrial brown fat uncoupling protein
• UniProt Gene Name: Ucp1
• UniProt Synonym Gene Names: Slc25a7; Ucp; UCP 1
• UniProt Entry Name: UCP1_MOUSE

Uniprot Description

UCP1: UCP are mitochondrial transporter proteins that create proton leaks across the inner mitochondrial membrane, thus uncoupling oxidative phosphorylation from ATP synthesis. As a result, energy is dissipated in the form of heat. Belongs to the mitochondrial carrier family.

Protein type: Membrane protein, multi-pass; Mitochondrial; Membrane protein, integral

Cellular Component: mitochondrial envelope; mitochondrion; membrane; mitochondrial inner membrane; mitochondrial membrane; integral to membrane

Molecular Function: protein binding; oxidative phosphorylation uncoupler activity

Biological Process: regulation of transcription from RNA polymerase II promoter; proton transport; transport; transmembrane transport; mitochondrial transport