Typical Testing Data/Standard Curve (for reference only)
Ammonia Assay Kit
Ammonia Assay Kit
Synonyms
Ammonia; Ammonia Assay Kit; Ammonia assay kit
Preparation and Storage
Store at 4 degree C.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for Ammonia assay kit
Principle of the Assay: Ammonia Assay Kit (Colorimetric) measures ammonia levels within urine or other biological samples. Samples are compared to a known concentration of ammonium chloride standard within a 96-well microtiter plate format. Samples and standards react with a chromogen in alkali solution to produce a blue-green colored product. After 30 minutes, the plate is read with a standard 96-well spectrophotometric microplate reader at an optical density between 630 nm and 670 nm (Figure 2). Higher OD values correlate with high ammonia concentrations. Sample ammonia concentrations are determined by comparison with the known ammonium chloride standards. The standard curve is linear up to 800 uM ammonia and the kit has a sensitivity of 12.5 uM ammonia.
Background: Ammonia is a compound composed of nitrogen and hydrogen (formula NH3). It is a common waste product in aquatic organisms, and serves as a nutritional staple of terrestrial organisms by functioning as a food/fertilizer precursor. Approximately 88% of ammonia is used around the world as fertilizer, anhydrously or as its salts or solutions. Ammonia is a building block for the synthesis of many pharmaceutical products as well as commercial cleaning products. Ammonia can be used to synthesize compounds such as ethanolamine, ethyl carbamate, ammonium perchlorate, ammonium nitrate, formamide, dinitrogen tetroxide, alprazolam, hexamethylenetetramine, and ammonium bicarbonate. In certain organisms, ammonia is synthesized from nitrogen found in the air by nitrogenase enzymes using a process called nitrogen fixation. Ammonia is also a resulting product of amino acid deamination catalyzed by enzymes such as glutamate dehydrogenase 1. Ammonia is commonly excreted in aquatic animals, while in humans ammonia is converted to the less toxic molecule urea. Urea is one of the main components of urine. Many birds, reptiles, and insects excrete nitrogenous waste as uric acid. Ammonia also functions in both normal and abnormal animal physiology: it is made by normal amino acid metabolism mechanisms and is toxic in high concentrations. The mammalian liver converts ammonia to urea through a series of enzymatic reactions called the urea cycle. In liver dysfunctions such as cirrhosis, elevated amounts of ammonia in the blood may result (hyperammonemia). At the same time, defects in the enzymes responsible for the urea cycle, such as ornithine transcarbamylase, lead to high ammonia levels. Hyperammonemia contributes to the symptoms of hepatic encephalopathy such as confusion and coma. In addition high ammonia levels can cause neurologic diseases observed in people with urea cycle defects and organic acidurias. Ammonia Assay Kit (Colorimetric) is based on a modified version of the Berthelot reaction. Ammonia reacts with an alkaline reagent to produce a blue-green colored product that can be measured with a standard spectrophotometric plate reader at an optical density between 630-670 nm. Each kit provides sufficient reagents to perform up to 100 assays, including blanks, ammonia standards and unknown samples.
Background: Ammonia is a compound composed of nitrogen and hydrogen (formula NH3). It is a common waste product in aquatic organisms, and serves as a nutritional staple of terrestrial organisms by functioning as a food/fertilizer precursor. Approximately 88% of ammonia is used around the world as fertilizer, anhydrously or as its salts or solutions. Ammonia is a building block for the synthesis of many pharmaceutical products as well as commercial cleaning products. Ammonia can be used to synthesize compounds such as ethanolamine, ethyl carbamate, ammonium perchlorate, ammonium nitrate, formamide, dinitrogen tetroxide, alprazolam, hexamethylenetetramine, and ammonium bicarbonate. In certain organisms, ammonia is synthesized from nitrogen found in the air by nitrogenase enzymes using a process called nitrogen fixation. Ammonia is also a resulting product of amino acid deamination catalyzed by enzymes such as glutamate dehydrogenase 1. Ammonia is commonly excreted in aquatic animals, while in humans ammonia is converted to the less toxic molecule urea. Urea is one of the main components of urine. Many birds, reptiles, and insects excrete nitrogenous waste as uric acid. Ammonia also functions in both normal and abnormal animal physiology: it is made by normal amino acid metabolism mechanisms and is toxic in high concentrations. The mammalian liver converts ammonia to urea through a series of enzymatic reactions called the urea cycle. In liver dysfunctions such as cirrhosis, elevated amounts of ammonia in the blood may result (hyperammonemia). At the same time, defects in the enzymes responsible for the urea cycle, such as ornithine transcarbamylase, lead to high ammonia levels. Hyperammonemia contributes to the symptoms of hepatic encephalopathy such as confusion and coma. In addition high ammonia levels can cause neurologic diseases observed in people with urea cycle defects and organic acidurias. Ammonia Assay Kit (Colorimetric) is based on a modified version of the Berthelot reaction. Ammonia reacts with an alkaline reagent to produce a blue-green colored product that can be measured with a standard spectrophotometric plate reader at an optical density between 630-670 nm. Each kit provides sufficient reagents to perform up to 100 assays, including blanks, ammonia standards and unknown samples.
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Product Notes
The Ammonia (Catalog #AAA169327) is an Assay Kit and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "Ammonia, Assay Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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