Typical Testing Data/Standard Curve (for reference only)
Mouse uncoupling protein 1 (mitochondrial, proton carrier) ELISA Kit | UCP1 elisa kit
Mouse Mitochondrial brown fat uncoupling protein 1, UCP1 ELISA Kit
Gene Names
Ucp1; Ucp; Slc25a7; AI385626
Reactivity
Mouse
Synonyms
uncoupling protein 1 (mitochondrial; proton carrier); Mouse Mitochondrial brown fat uncoupling protein 1; UCP1 ELISA Kit; Mouse Mitochondrial brown fat uncoupling protein 1 (UCP1) ELISA kit; SLC25A7; UCP; mitochondrial brown fat uncoupling protein; thermogenin; uncoupling protein 1; UCP1 elisa kit
Reactivity
Mouse
Specificity
This assay has high sensitivity and excellent specificity for detection of mouse UCP1. No significant cross-reactivity or interference between mouse UCP1 and analogues was observed.
Sequence Length
307
Samples
Serum, plasma, tissue homogenates, cell lysates
Assay Type
Quantitative Sandwich
Detection Range
4.68 pg/ml-300 pg/ml
Sensitivity
< 1.17 pg/ml
Intra-assay Precision
Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision
Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
Preparation and Storage
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for UCP1 elisa kit
Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for UCP1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any UCP1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for UCP1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of UCP1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
33,248 Da
NCBI Official Full Name
mitochondrial brown fat uncoupling protein 1
NCBI Official Synonym Full Names
uncoupling protein 1 (mitochondrial, proton carrier)
NCBI Official Symbol
Ucp1
NCBI Official Synonym Symbols
Ucp; Slc25a7; AI385626
NCBI Protein Information
mitochondrial brown fat uncoupling protein 1; UCP 1; thermogenin; solute carrier family 25 member 7; uncoupling protein, mitochondrial; uncoupling protein 1, mitochondrial
UniProt Protein Name
Mitochondrial brown fat uncoupling protein 1
Protein Family
UniProt Gene Name
Ucp1
UniProt Synonym Gene Names
Slc25a7; Ucp; UCP 1
UniProt Entry Name
UCP1_MOUSE
Uniprot Description
UCP1: UCP are mitochondrial transporter proteins that create proton leaks across the inner mitochondrial membrane, thus uncoupling oxidative phosphorylation from ATP synthesis. As a result, energy is dissipated in the form of heat. Belongs to the mitochondrial carrier family.
Protein type: Membrane protein, integral; Membrane protein, multi-pass; Mitochondrial
Cellular Component: mitochondrial envelope; membrane; mitochondrion; mitochondrial membrane; mitochondrial inner membrane; integral to membrane
Molecular Function: protein binding; oxidative phosphorylation uncoupler activity
Biological Process: regulation of transcription from RNA polymerase II promoter; proton transport; transport; transmembrane transport; mitochondrial transport
Research Articles on UCP1
Similar Products
Product Notes
The Mouse UCP1 ucp1 (Catalog #AAA933377) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA933377 ELISA Kit recognizes Mouse UCP1. It is sometimes possible for the material contained within the vial of "uncoupling protein 1 (mitochondrial, proton carrier), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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