Ubiquitin Carboxyl-Terminal Hydrolase Isozyme L3/UCH-L3 Recombinant Protein | UCH-L3 recombinant protein

Recombinant Human Ubiquitin Carboxyl-Terminal Hydrolase Isozyme L3/UCH-L3 Protein (C-6His)

Cat. Num. AAA2553342

• Gene Names: UCHL3; UCH-L3
• Purity: >95% as determined by reducing SDS-PAGE.
• Synonyms: Ubiquitin Carboxyl-Terminal Hydrolase Isozyme L3/UCH-L3; Recombinant Human Ubiquitin Carboxyl-Terminal Hydrolase Isozyme L3/UCH-L3 Protein (C-6His); Ubiquitin Carboxyl-Terminal Hydrolase Isozyme L3; UCH-L3; Ubiquitin Thioesterase L3; UCHL3; UCH-L3 recombinant protein

• Host: E Coli
• Purity/Purification: >95% as determined by reducing SDS-PAGE.
• Form/Format: Supplied as a 0.2 mum filtered solution of 50mM Tris HCl, 150mM NaCl, 1mM DTT, 50% Glycerol, pH 8.0.
• Sequence Positions: Met1-Ala230
• Sequence Length: 194

• Species: Human
• Endotoxin: <1.0 EU per ug as determined by LAL test.
• Fusion Tag: C-6His
• Preparation and Storage: Store at < -20 degree C, stable for 6 months after receipt. Please minimize freeze-thaw cycles.

Related Product Information for UCH-L3 recombinant protein

Ubiquitin Carboxyl-Terminal Hydrolases (UCHs) are a family of cysteine hydrolases. They catalyze the hydrolysis of amides, thioesters and esters, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin. Up regulation of UCHL3 is associated with uterine cervical neoplasms. UCHL3 is implicated in age related cognitive disorders. UCHL3 also promotes adipogenesis and insulin signaling. In mice, UCHL3 knockout have been shown to be resistant to diet-induced obesity.

NCBI and Uniprot Product Information

• NCBI GI #: 401709950
• NCBI GeneID: 7347
• NCBI Accession #: NP_001257881.1
• NCBI GenBank Nucleotide #: NM_001270952.1
• UniProt Accession #: P15374; Q5TBK8; Q6IBE9; B2R970
• Molecular Weight: Molecular Mass: 27.3 kDa; Actual Protein Molecular Mass: 25 kDa
• NCBI Official Full Name: ubiquitin carboxyl-terminal hydrolase isozyme L3 isoform 1
• NCBI Official Synonym Full Names: ubiquitin C-terminal hydrolase L3
• NCBI Official Symbol: UCHL3
• NCBI Official Synonym Symbols: UCH-L3
• NCBI Protein Information: ubiquitin carboxyl-terminal hydrolase isozyme L3
• UniProt Protein Name: Ubiquitin carboxyl-terminal hydrolase isozyme L3
• UniProt Gene Name: UCHL3
• UniProt Synonym Gene Names: UCH-L3

NCBI Description

The protein encoded by this gene is a member of the deubiquitinating enzyme family. Members of this family are proteases that catalyze the removal of ubiquitin from polypeptides and are divided into five classes, depending on the mechanism of catalysis. This protein may hydrolyze the ubiquitinyl-N-epsilon amide bond of ubiquitinated proteins to regenerate ubiquitin for another catalytic cycle. Alternative splicing results in multiple transcript variants that encode different protein isoforms. [provided by RefSeq, Aug 2012]

Uniprot Description

Deubiquitinating enzyme (DUB) that controls levels of cellular ubiquitin through processing of ubiquitin precursors and ubiquitinated proteins. Thiol protease that recognizes and hydrolyzes a peptide bond at the C-terminal glycine of either ubiquitin or NEDD8. Has a 10-fold preference for Arg and Lys at position P3", and exhibits a preference towards 'Lys-48'-linked ubiquitin chains. Deubiquitinates ENAC in apical compartments, thereby regulating apical membrane recycling. Indirectly increases the phosphorylation of IGFIR, AKT and FOXO1 and promotes insulin-signaling and insulin-induced adipogenesis. Required for stress-response retinal, skeletal muscle and germ cell maintenance. May be involved in working memory. Can hydrolyze UBB(+1), a mutated form of ubiquitin which is not effectively degraded by the proteasome and is associated with neurogenerative disorders.