Dectin-2 Active Protein | CLEC6A active protein

Dectin-2 Protein, Mouse, Recombinant (His Tag)

Cat. Num. AAA8122648

• Gene Names: Clec4n; Nkcl; Clec6a; Clecsf10
• Purity: >97% as determined by SDS-PAGE
• Synonyms: Dectin-2; Dectin-2 Protein; Mouse; Recombinant (His Tag); Mouse CLEC4N/CLEC6A/Dectin-2 Protein (His Tag); Clec4n Protein; Clecsf10 Protein; Nkcl Protein; C-type lectin domain family 6 member A; CLEC6A active protein

• Host: HEK293 Cells
• Purity/Purification: >97% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Ile44-Leu209

• Species: Mouse
• Activity: Measured by its ability to agglutinate human red blood cells. Mouse CLEC4N at 25ug/ml could agglutinate 1% HRBC.
• Endotoxin: <1.0EU per ug of the protein as determined by the LAL method
• Predicted N Terminal: Ile 44
• Tag: C-His
• Protein Construction: A DNA sequence encoding the mouse CLEC4N (NP_064385.1) extracellular domain (Ile 44-Leu 209) was expressed, with a C-terminal polyhistidine tag.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for CLEC6A active protein

Background: C-type lectin domain family 4 member N (CLEC4N), also known as Dectin-2, is a C-type lectin expressed by dendritic cells (DCs) and macrophages. Members of the C-type lectin domain (CTLD) superfamily are metazoan proteins functionally important in glycoprotein metabolism, mechanisms of multicellular integration and immunity. They share a common fold and are involved in a variety of functions, such as generalized defense mechanisms against foreign agents, discrimination between healthy and pathogen-infected cells, and endocytosis and blood coagulation. Genome-level studies on human, elegans and melanogaster demonstrated almost complete divergence among invertebrate and mammalian families of CTLD-containing proteins (CTLDcps). The vertebrate CTLDcp family was essentially formed early in vertebrate evolution and is completely different from the invertebrate families. The composition of the CTLDcp superfamily in fish and mammals suggests that large scale duplication events played an important role in the evolution of vertebrates. Dectin-2 is important in host defense against C. albicans by inducing Th17 cell differentiation. Dectin-2 constitutes a major fungal pattern recognition receptor (PRR) that can couple to the Syk-CARD9 innate signaling pathway to activate DCs and regulate adaptive immune responses to fungal infection.

References

Robinson MJ, et al. (2009) Dectin-2 is a Syk-coupled pattern recognition receptor crucial for Th17 responses to fungal infection. J Exp Med. 206(9): 2037-51.Saijo S, et al. (2010) Dectin-2 recognition of alpha-mannans and induction of Th17 cell differentiation is essential for host defense against Candida albicans. Immunity. 32(5): 681-91.

NCBI and Uniprot Product Information

• NCBI GI #: 9910158
• NCBI GeneID: 56620
• NCBI Accession #: NP_064385.1
• NCBI GenBank Nucleotide #: NM_020001.2
• UniProt Accession #: Q9JKF4; Q9JKF2; Q9JKF3
• Molecular Weight: 19,332 Da
• NCBI Official Full Name: C-type lectin domain family 6 member A isoform 1
• NCBI Official Synonym Full Names: C-type lectin domain family 4, member n
• NCBI Official Symbol: Clec4n
• NCBI Official Synonym Symbols: Nkcl; Clec6a; Clecsf10
• NCBI Protein Information: C-type lectin domain family 6 member A
• UniProt Protein Name: C-type lectin domain family 6 member A
• Protein Family: C-type lectin domain family
• UniProt Gene Name: Clec6a
• UniProt Synonym Gene Names: Clec4n; Clecsf10; Dectin2; DC-associated C-type lectin 2; Dectin-2
• UniProt Entry Name: CLC6A_MOUSE