Rabbit anti-Human, Mouse Tbx21 Polyclonal Antibody | anti-Tbx21 antibody

Tbx21 Polyclonal Antibody

Cat. Num. AAA3013541

• Gene Names: Tbx21; TBT1; Tbet; Tblym
• Reactivity: Human, Mouse
• Applications: Western Blot, Flow Cytometry, Functional Assay
• Purity: Peptide affinity purified.
• Synonyms: Tbx21; Polyclonal Antibody; Tbx21 Polyclonal Antibody; T-box protein 21; Tbet; Tblym; T bet; T box 21; T box expressed in T cells; T box protein 21; T box transcription factor TBX21; T cell specific T box transcription factor; T cell specific T box transcription factor T bet; T PET; T-box transcription factor TBX21; T-cell-specific T-box transcription factor T-bet; TBET; TBLYM; TBX 21; TBX21_HUMAN antibody TPET; Transcription factor TBLYM; anti-Tbx21 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Tbx21 polyclonal antibody detects endogenous levels of Tbx21 protein.
• Purity/Purification: Peptide affinity purified.
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 530

• Applicable Applications for anti-Tbx21 antibody: Western Blot (WB), Flow Cytometry (FC/FACS)
• Application Notes: WB: 1:500; FC/FACS: 1:50-1:100
• Immunogen: Synthetic peptide within human T-bet aa 480-530.
• Preparation and Storage: Store at 4 degree C after thawing.; Aliquot store at -20 degree C.; Avoid repeated freeze/thaw cycles.

Western Blot (WB)

(Western Blot analysis of T-bet on mouse marrow tissue lysate using anti-T-bet antibody at 1/500 dilution.)

Flow Cytometry (FC/FACS)

(Flow cytometric analysis of Jurkat cells with T-bet antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.)

Related Product Information for anti-Tbx21 antibody

T helper (Th) lymphocytes differentiate into two unique subsets, Th1 and Th2, which differ both in function and in the cytokines they secrete. Th1 and Th2 cytokines promote the growth and differentiation of their subset, and inhibit the growth and differentiation of the opposing subset. T-bet (T box expressed in T cells) is a Th1-specific T box transcription factor that controls the expres-sion of the Th1 cytokine, IFN-gamma. T-bet also converts effector Th2 cells into the opposing Th1 subset. T-bet is selectively expressed in Th1 cells. The level of T-bet expression is increased by signals mediated by the T cell receptor (TCR). IL-12 also induces an increase in the level of T-bet. T-bet was originally isolated from nuclear extracts of resting and PMA/ionomycin-activated AE7 cells. T-bet is expressed in low levels in AE7 cells, and in increased levels in stimulated AE7.

NCBI and Uniprot Product Information

• NCBI GI #: 247269497
• NCBI GeneID: 57765
• NCBI Accession #: NP_062380.2
• NCBI GenBank Nucleotide #: NP_062380.2
• UniProt Accession #: Q9JKD8
• Molecular Weight: 58kDa
• NCBI Official Full Name: T-box transcription factor TBX21
• NCBI Official Synonym Full Names: T-box 21
• NCBI Official Symbol: Tbx21
• NCBI Official Synonym Symbols: TBT1; Tbet; Tblym
• NCBI Protein Information: T-box transcription factor TBX21
• UniProt Protein Name: T-box transcription factor TBX21
• Protein Family: T-box transcription factor
• UniProt Gene Name: Tbx21
• UniProt Synonym Gene Names: Tbet; Tblym; T-box protein 21
• UniProt Entry Name: TBX21_MOUSE