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(Purity: Sample Purity Data. For specific information on a given lot, see related technical data sheet.)
Ubiquitin recombinant protein
Ubiquitin Protein
Applications
Western Blot
Synonyms
Ubiquitin; Ubiquitin Protein; UBB; MGC8385; FLJ25987; Ubiquitin recombinant protein
Host
E Coli
Form/Format
Recombinant protein stored in 20mM Tris -HCl pH7.0, 150mM NaCl, 0.1mM EDTA, 1mM DTT and 25% glycerol.
Applicable Applications for Ubiquitin recombinant protein
Enzyme Assay, Western Blot (WB)
Tag
TAG-Free
Species
Human
Preparation and Storage
Store product at -70 degree C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
SDS-Page
(Purity: Sample Purity Data. For specific information on a given lot, see related technical data sheet.)
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(Purity: Sample Purity Data. For specific information on a given lot, see related technical data sheet.)
Related Product Information for Ubiquitin recombinant protein
Recombinant human tag free ubiquitin (1-76) protein was expressed in E Coli cells. The gene accession number is NM_018955.
Ubiquitin is a highly-conserved protein that is widely expressed in eukaryotes (1). Ubiquitin is required for the rapid proteasomal degradation of intracellular proteins in an ATP-dependent manner. UBB+1 is a frameshift mutant form of ubiquitin, which not only inhibits the function of the proteasome machinery, but also accumulates in the cells associated with tauopathies, including Alzheimer's disease and polyglutamine diseases because of the cell's inability to clear cytotoxic misfolded amyloid-beta and phosphorylated-tau (2). UBB+1 also serves as an endogenous marker for proteasomal dysfunction in liver disease and muscle disease, where the protein may also have a role in pathogenesis.
Ubiquitin is a highly-conserved protein that is widely expressed in eukaryotes (1). Ubiquitin is required for the rapid proteasomal degradation of intracellular proteins in an ATP-dependent manner. UBB+1 is a frameshift mutant form of ubiquitin, which not only inhibits the function of the proteasome machinery, but also accumulates in the cells associated with tauopathies, including Alzheimer's disease and polyglutamine diseases because of the cell's inability to clear cytotoxic misfolded amyloid-beta and phosphorylated-tau (2). UBB+1 also serves as an endogenous marker for proteasomal dysfunction in liver disease and muscle disease, where the protein may also have a role in pathogenesis.
Product Categories/Family for Ubiquitin recombinant protein
References
1. Baker, R T. et al: The human ubiquitin gene family: structure of a gene and pseudogenes from the Ub B subfamily. Nucleic Acids Res.15: 443-463, 1987.
2. Fratta, P. et al: Mutant ubiquitin UBB+1 is accumulated in sporadic inclusion-body myositis muscle fibers. Neurology. 2004 Sep 28;63(6):1114-7.
2. Fratta, P. et al: Mutant ubiquitin UBB+1 is accumulated in sporadic inclusion-body myositis muscle fibers. Neurology. 2004 Sep 28;63(6):1114-7.
NCBI and Uniprot Product Information
Similar Products
Product Notes
The Ubiquitin (Catalog #AAA517737) is a Recombinant Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Ubiquitin can be used in a range of immunoassay formats including, but not limited to, Enzyme Assay, Western Blot (WB). Researchers should empirically determine the suitability of the Ubiquitin for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Ubiquitin, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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