Activity
(Specific Activity: Sample Activity Plot. For specific information on a given lot, see related technical data sheet.)
UCHL3 recombinant protein
UCHL3, Active
Applications
Western Blot
Synonyms
UCHL3; Active; UCH-L3; UCHL3 recombinant protein
Host
E Coli
Form/Format
Recombinant protein stored in 50mM sodium phosphate, pH7.0, 300mM NaCl, 150mM imidazole, 0.1mM PMSF, 0.25mM DTT, 25% glycerol.
Applicable Applications for UCHL3 recombinant protein
Activity Assay, Western Blot (WB)
Tag
HIS
Species
Human
Preparation and Storage
Store product at -70 degree C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Activity
(Specific Activity: Sample Activity Plot. For specific information on a given lot, see related technical data sheet.)
Activity
(Specific Activity: Sample Activity Plot. For specific information on a given lot, see related technical data sheet.)
SDS-Page
(Purity: Sample Purity Data. For specific information on a given lot, see related technical data sheet.)
SDS-Page
(Purity: Sample Purity Data. For specific information on a given lot, see related technical data sheet.)
Related Product Information for UCHL3 recombinant protein
Recombinant full length human UCHL3, was expressed in E Coli cells using an N-terminal His tag. The UCHL3 gene accession number is NM_006002.
UCHL3 (Ubiquitin carboxyl-terminal hydrolase isozyme L3) is a thiol protease belonging to the deubiquitinating enzymes family and catalyzes the removal of ubiquitin from polypeptides. UCHL3 takes part in the regulation of apical membrane recycling by deubiquitinating ENAC in apical compartments (1). UCHL3 was found to hydrolyze mutant ubiquitin (UBB+1), a mutation that is associated with neurodegenerative diseases (2).
UCHL3 (Ubiquitin carboxyl-terminal hydrolase isozyme L3) is a thiol protease belonging to the deubiquitinating enzymes family and catalyzes the removal of ubiquitin from polypeptides. UCHL3 takes part in the regulation of apical membrane recycling by deubiquitinating ENAC in apical compartments (1). UCHL3 was found to hydrolyze mutant ubiquitin (UBB+1), a mutation that is associated with neurodegenerative diseases (2).
Product Categories/Family for UCHL3 recombinant protein
References
1. Butterworth, M.B. et al: The deubiquitinating enzyme UCH-L3 regulates the apical membrane recycling of the epithelial sodium channel. J Biol Chem. 282(52):37885-93, 2007.
2. Dennissen, F.J. et al: Mutant ubiquitin (UBB+1) associated with neurodegenerative disorders is hydrolyzed by ubiquitin C-terminal hydrolase L3 (UCH-L3). FEBS Letters 585:1873-3468, 2011.
2. Dennissen, F.J. et al: Mutant ubiquitin (UBB+1) associated with neurodegenerative disorders is hydrolyzed by ubiquitin C-terminal hydrolase L3 (UCH-L3). FEBS Letters 585:1873-3468, 2011.
NCBI and Uniprot Product Information
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
27kDa
Protein Family
Similar Products
Product Notes
The UCHL3 (Catalog #AAA517770) is a Recombinant Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's UCHL3 can be used in a range of immunoassay formats including, but not limited to, Activity Assay, Western Blot (WB). Researchers should empirically determine the suitability of the UCHL3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "UCHL3, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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