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(Purity: Sample Purity Data. For specific information on a given lot, see related technical data sheet.)
RhoA Mutant (S188A) Recombinant Protein | RhoA recombinant protein
RhoA Mutant (S188A) Protein
Applications
Western Blot
Synonyms
RhoA Mutant (S188A); RhoA Mutant (S188A) Protein; ARH12; ARHA; RHO12; RHOH12; RhoA recombinant protein
Host
E Coli
Form/Format
Recombinant protein stored in 50mM sodium phosphate, pH7.0, 300mM NaCl, 150mM imidazole, 0.1mM PMSF, 0.25mM DTT, and 25% glycerol.
Applicable Applications for RhoA recombinant protein
Kinase Assay, Western Blot (WB)
Tag
HIS
Species
Human
Preparation and Storage
Store product at -70 degree C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
SDS-Page
(Purity: Sample Purity Data. For specific information on a given lot, see related technical data sheet.)
SDS-Page
(Purity: Sample Purity Data. For specific information on a given lot, see related technical data sheet.)
Related Product Information for RhoA recombinant protein
Recombinant human RhoA (S188A)(1-190) was expressed in E Coli cells using an N-terminal His tag. The gene accession number is NM_001664.
RhoA is a member of the Rho gene family that interacts with protein kinases and serves as a target of activated GTPase. RhoA play an important role in the initial events of cell protrusion, whereas Rac1 and Cdc42 activate pathways implicated in reinforcement and stabilization of newly expanded protrusions. Rho mediates corneal epithelial migration in response to external stimuli by regulating the organization of the actin cytoskeleton (1). The inhibition of RhoA signaling is required for both vaccinia morphogenesis and virus-induced cell motility. Vaccinia inhibits RhoA signalling using the viral protein F11 and this can enhance the spread of infection (2). It has been reported that cGMP/cGMP-dependent protein kinase can phosphorylate RhoA at Ser188 in vitro (3).
RhoA is a member of the Rho gene family that interacts with protein kinases and serves as a target of activated GTPase. RhoA play an important role in the initial events of cell protrusion, whereas Rac1 and Cdc42 activate pathways implicated in reinforcement and stabilization of newly expanded protrusions. Rho mediates corneal epithelial migration in response to external stimuli by regulating the organization of the actin cytoskeleton (1). The inhibition of RhoA signaling is required for both vaccinia morphogenesis and virus-induced cell motility. Vaccinia inhibits RhoA signalling using the viral protein F11 and this can enhance the spread of infection (2). It has been reported that cGMP/cGMP-dependent protein kinase can phosphorylate RhoA at Ser188 in vitro (3).
Product Categories/Family for RhoA recombinant protein
References
1. Wu, K. Y.et.al: Local translation of RhoA regulates growth cone collapse. Nature 436: 1020-1024, 2005.
2. Valderrama, F.et.al: Vaccinia virus-induced cell motility requires F11L-mediated inhibition of RhoA signaling. Science 311: 377-381, 2006.
3. Sawada N. et.al: cGMP-dependent protein kinase phosphorylates and inactivates RhoA. Biochem. Biophys. Res. Commun. 280:798-805(2001).
2. Valderrama, F.et.al: Vaccinia virus-induced cell motility requires F11L-mediated inhibition of RhoA signaling. Science 311: 377-381, 2006.
3. Sawada N. et.al: cGMP-dependent protein kinase phosphorylates and inactivates RhoA. Biochem. Biophys. Res. Commun. 280:798-805(2001).
NCBI and Uniprot Product Information
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
23kDa
Protein Family
Similar Products
Product Notes
The RhoA (Catalog #AAA517561) is a Recombinant Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's RhoA Mutant (S188A) can be used in a range of immunoassay formats including, but not limited to, Kinase Assay, Western Blot (WB). Researchers should empirically determine the suitability of the RhoA for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "RhoA Mutant (S188A), Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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