Ubiquitin Conjugating Enzyme E2W Recombinant Protein | UBE2W recombinant protein

Recombinant Human Ubiquitin Conjugating Enzyme E2W

Cat. Num. AAA144561

• Gene Names: UBE2W; UBC16; UBC-16
• Purity: Greater than 90.0% as determined by SDS-PAGE.
• Synonyms: Ubiquitin Conjugating Enzyme E2W; Recombinant Human Ubiquitin Conjugating Enzyme E2W; UBE2W Human; Ubiquitin Conjugating Enzyme E2W Human Recombinant; Probable ubiquitin-conjugating enzyme E2 W; Ubiquitin carrier protein W; Ubiquitin-protein ligase W; UBE2W; hUBC-16; FLJ11011; UBE2W recombinant protein

• Host: E Coli
• Purity/Purification: Greater than 90.0% as determined by SDS-PAGE.
• Form/Format: UBE2W solution (1mg/ml) containing 20mM Tris-HCl buffer (pH 8.0), 10% glycerol, 2mM DTT and 0.1M NaCl.; Sterile filtered colorless solution.
• Sequence: MGSSHHHHHH SSGLVPRGSH MASMQKRLQK ELLALQNDPP PGMTLNEKSV QNSITQWIVD MEGAPGTLYE GEKFQLLFKF SSRYPFDSPQ VMFTGENIPV HPHVYSNGHI CLSILTEDWS PALSVQSVCL SIISMLSSCK EKRRPPDNSF YVRTCNKNPK KTKWWYHDDT C.
• Sequence Length: 191

• Preparation and Storage: UBE2W Human Recombinant although stable at 4 degree C for 1 week, should be stored below -18 degree C. Please prevent freeze thaw cycles.

Related Product Information for UBE2W recombinant protein

Description: UBE2W produced in E Coli is a single, non-glycosylated polypeptide chain containing 171 amino acids (1-151 a.a.) and having a molecular mass of 19.5kDa.UBE2W is fused to a 20 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Introduction: UBE2W receives ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro, UBE2W catalyzes monoubiquitination and 'Lys-11'-linked polyubiquitination. UBE2W is broadly expressed, particularly at highest levels in the testis.

Product Categories/Family for UBE2W recombinant protein

Enzymes; Ubiquitin Conjugating Enzyme

NCBI and Uniprot Product Information

• NCBI GI #: 402169567
• NCBI GeneID: 55284
• NCBI Accession #: NP_001001481.2
• NCBI GenBank Nucleotide #: NM_001001481.2
• UniProt Accession #: Q96B02; Q1XBE0; Q9H823; Q9HAG6; Q9NV07; B4DIV1
• Molecular Weight: 20,689 Da
• NCBI Official Full Name: ubiquitin-conjugating enzyme E2 W isoform 1
• NCBI Official Synonym Full Names: ubiquitin-conjugating enzyme E2W (putative)
• NCBI Official Symbol: UBE2W
• NCBI Official Synonym Symbols: UBC16; UBC-16
• NCBI Protein Information: ubiquitin-conjugating enzyme E2 W; N-terminus-conjugating E2; ubiquitin carrier protein W; ubiquitin-conjugating enzyme 16; ubiquitin-conjugating enzyme 2W; ubiquitin-protein ligase W
• UniProt Protein Name: Ubiquitin-conjugating enzyme E2 W
• Protein Family: Ubiquitin-conjugating enzyme
• UniProt Gene Name: UBE2W
• UniProt Synonym Gene Names: UBC16; UBC-16
• UniProt Entry Name: UBE2W_HUMAN

NCBI Description

This gene encodes a nuclear-localized ubiquitin-conjugating enzyme (E2) that, along with ubiquitin-activating (E1) and ligating (E3) enzymes, coordinates the addition of a ubiquitin moiety to existing proteins. The encoded protein promotes the ubiquitination of Fanconi anemia complementation group proteins and may be important in the repair of DNA damage. There is a pseudogene for this gene on chromosome 1. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2012]

Uniprot Description

UBE2W: Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. Catalyzes monoubiquitination. Involved in degradation of misfolded chaperone substrates by mediating monoubiquitination of STUB1/CHIP, leading to recruitment of ATXN3 to monoubiquitinated STUB1/CHIP, and restriction of the length of ubiquitin chain attached to STUB1/CHIP substrates by ATXN3. After UV irradiation, but not after mitomycin-C (MMC) treatment, acts as a specific E2 ubiquitin-conjugating enzyme for the Fanconi anemia complex by associating with E3 ubiquitin-protein ligase FANCL and catalyzing monoubiquitination of FANCD2, a key step in the DNA damage pathway. In vitro catalyzes 'Lys-11'-linked polyubiquitination. Belongs to the ubiquitin-conjugating enzyme family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Ubiquitin conjugating system; EC 6.3.2.19; Enzyme, misc.; Ubiquitin ligase; Ligase

Chromosomal Location of Human Ortholog: 8q21.11

Cellular Component: cytoplasm; nucleus

Molecular Function: small conjugating protein ligase activity; ubiquitin protein ligase binding; ubiquitin-protein ligase activity; ATP binding; ligase activity

Biological Process: protein monoubiquitination; proteasomal ubiquitin-dependent protein catabolic process; misfolded or incompletely synthesized protein catabolic process; DNA repair