uBE2T recombinant protein

uBE2T, 1-197aa, Human, His tag, E Coli

Cat. Num. AAA203589

• Gene Names: UBE2T; FANCT; PIG50; HSPC150
• Applications: SDS-Page
• Purity: > 95% by SDS-PAGE
• Synonyms: uBE2T; 1-197aa; Human; His tag; E Coli; ubiquitin-conjugating enzyme E2T; HSPC150; PIG50; ubiquitin-conjugating enzyme E2T Cell proliferation inducing gene 50 protein; HSPC150 protein similar to ubiquitin conjugating enzyme; HSPC150 protein similar to ubiquitin-conjugating enzyme; ubiquitin carrier protein T; ubiquitin conjugating enzyme; ubiquitin conjugating enzyme E2T; ubiquitin protein ligase T; ; uBE2T recombinant protein

• Host: E Coli
• Purity/Purification: > 95% by SDS-PAGE
• Form/Format: Liquid. In 20mM Tris-HCl Buffer (pH 8.0) containing 10% glycerol 1mM DTT.
• Concentration: 1 mg/ml (determined by Bradford assay) (varies by lot)
• Sequence: MQRASRLKRE LHMLATEPPP GITCWQDKDQ MDDLRAQILG GANTPYEKGV FKLEVIIPER YPFEPPQIRF LTPIYHPNID SAGRICLDVL KLPPKGAWRP SLNIATVLTS IQLLMSEPNP DDPLMADISS EFKYNKPAFL KNARQWTEKH ARQKQKADEE EMLDNLPEAG DSRVHNSTQK RKASQLVGIE KKFHPDVLEH HHHHH
• Sequence Length: 197

• Applicable Applications for uBE2T recombinant protein: SDS-PAGE
• Antigen Species: Human
• Tag Information: His-tag
• Preparation and Storage: Can be stored at 4 degree C short term (1-2 weeks).; For long term storage, aliquot and store at -20 degree C or -70 degree C.; Avoid repeated freezing and thawing cycles.

SDS-Page

Related Product Information for uBE2T recombinant protein

uBE2T, also known as PIG50 or HSPC150, is a member of the E2 ubiquitin-conjugating enzyme family Involved in the protein degradation pathway. This protein catalyzes the ATP-dependent attachment of ubiquitin (ub) to target proteins, thereby tagging them for subsequent destruction by the proteasome. Additionally, uBE2T is thought to be a crucial component of the Faconi anemia pathway of DNA damage repair and, upon self-inactivation, may negatively regulate the Faconi pathway. Recombinant uBE2T protein, fused to His-tag at C-terminus, was expressed in E Coli and purified by using conventional chromatography techniques.

Product Categories/Family for uBE2T recombinant protein

Ubiquitination

References

Hao J., et al. (2008) Tumour Biol. 29 (3):195-203. ; Machida YJ., et al. (2006) Mol Cell. 23 (4):589-96. ;

NCBI and Uniprot Product Information

• NCBI GI #: 7661808
• NCBI GeneID: 29089
• NCBI Accession #: NP_054895
• NCBI GenBank Nucleotide #: NM_014176.3
• UniProt Accession #: Q9NPD8; Q2TU36
• Molecular Weight: 23.6 kDa (205aa), confirmed by MALDI-TOF
• NCBI Official Full Name: ubiquitin-conjugating enzyme E2 T isoform 1
• NCBI Official Synonym Full Names: ubiquitin conjugating enzyme E2 T
• NCBI Official Symbol: UBE2T
• NCBI Official Synonym Symbols: FANCT; PIG50; HSPC150
• NCBI Protein Information: ubiquitin-conjugating enzyme E2 T
• UniProt Protein Name: Ubiquitin-conjugating enzyme E2 T
• Protein Family: Ubiquitin-conjugating enzyme
• UniProt Gene Name: UBE2T

NCBI Description

The protein encoded by this gene catalyzes the covalent attachment of ubiquitin to protein substrates. Defects in this gene have been associated with Fanconi anemia of complementation group T. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2015]

Uniprot Description

Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. Catalyzes monoubiquitination. Involved in mitomycin-C (MMC)-induced DNA repair. Acts as a specific E2 ubiquitin-conjugating enzyme for the Fanconi anemia complex by associating with E3 ubiquitin-protein ligase FANCL and catalyzing monoubiquitination of FANCD2, a key step in the DNA damage pathway (PubMed:16916645, PubMed:17938197, PubMed:19111657, PubMed:19589784, PubMed:28437106). Also mediates monoubiquitination of FANCL and FANCI (PubMed:16916645, PubMed:17938197, PubMed:19111657, PubMed:19589784). May contribute to ubiquitination and degradation of BRCA1 (PubMed:19887602). In vitro able to promote polyubiquitination using all 7 ubiquitin Lys residues, but may prefer 'Lys-11'-, 'Lys-27'-, 'Lys-48'- and 'Lys-63'-linked polyubiquitination (PubMed:20061386).