UBE2T recombinant protein

UBE2T Protein, Human, Recombinant (His Tag)

Cat. Num. AAA8121168

• Gene Names: UBE2T; PIG50; HSPC150
• Purity: >90% as determined by SDS-PAGE
• Synonyms: UBE2T; UBE2T Protein; Human; Recombinant (His Tag); Human UBE2T/HSPC150 Protein (His Tag); FANCT Protein; HSPC150 Protein; PIG50 Protein; ubiquitin-conjugating enzyme E2T; UBE2T recombinant protein

• Host: E Coli
• Purity/Purification: >90% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, 30% glycerol, 0.1% Tween 20, 0.4mM Chaps, pH7.5. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Met1-Val197

• Species: Human
• Predicted N Terminal: Met
• Tag: C-His
• Protein Construction: A DNA sequence encoding the human UBE2T (Q9NPD8)(Met1-Val197) was fused with a polyhistidine tag at the C-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for UBE2T recombinant protein

Background: Ube2T is the E2 ubiquitin-conjugating enzyme of the Fanconi anemia DNA repair pathway and it is overexpressed in several cancers, representing an attractive target for the development of inhibitors. Notably, UBE2T locates at 1q32.1, and the gain of 1q is frequently observed in a variety of cancers. For instance, UBE2T serves an important role in the growth of bladder cancer cells, and may be considered as a potential biomarker and therapeutic target for bladder cancer.

NCBI and Uniprot Product Information

• NCBI GI #: 7661808
• NCBI GeneID: 29089
• NCBI Accession #: NP_054895.1
• NCBI GenBank Nucleotide #: NM_014176.3
• UniProt Accession #: Q9NPD8; Q2TU36
• Molecular Weight: 22,521 Da
• NCBI Official Full Name: ubiquitin-conjugating enzyme E2 T
• NCBI Official Synonym Full Names: ubiquitin-conjugating enzyme E2T (putative)
• NCBI Official Symbol: UBE2T
• NCBI Official Synonym Symbols: PIG50; HSPC150
• NCBI Protein Information: ubiquitin-conjugating enzyme E2 T; ubiquitin-protein ligase T; ubiquitin carrier protein T; ubiquitin conjugating enzyme; cell proliferation-inducing gene 50 protein; HSPC150 protein similar to ubiquitin-conjugating enzyme
• UniProt Protein Name: Ubiquitin-conjugating enzyme E2 T
• Protein Family: Ubiquitin-conjugating enzyme
• UniProt Gene Name: UBE2T
• UniProt Entry Name: UBE2T_HUMAN

NCBI Description

The covalent conjugation of ubiquitin to proteins regulates diverse cellular pathways and proteins. Ubiquitin is transferred to a target protein through a concerted action of a ubiquitin-activating enzyme (E1), a ubiquitin-conjugating enzyme (E2), such as UBE2T, and a ubiquitin ligase (E3) (Machida et al., 2006 [PubMed 16916645]).[supplied by OMIM, Mar 2008]

Uniprot Description

UBE2T: Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. Catalyzes monoubiquitination. Involved in mitomycin-C (MMC)-induced DNA repair: acts as a specific E2 ubiquitin-conjugating enzyme for the Fanconi anemia complex by associating with E3 ubiquitin-protein ligase FANCL and catalyzing monoubiquitination of FANCD2, a key step in the DNA damage pathway. Also mediates monoubiquitination of FANCL and FANCI. May contribute to ubiquitination and degradation of BRCA1. In vitro able to promote polyubiquitination using all 7 ubiquitin Lys residues, but may prefer 'Lys-11'-, 'Lys-27'-, 'Lys-48'- and 'Lys-63'-linked polyubiquitination. Belongs to the ubiquitin-conjugating enzyme family.

Protein type: EC 6.3.2.19; Ligase; Ubiquitin ligase; Ubiquitin conjugating system

Chromosomal Location of Human Ortholog: 1q32.1

Cellular Component: nucleoplasm; cytoplasm; nucleus

Molecular Function: small conjugating protein ligase activity; ubiquitin protein ligase binding; ubiquitin-protein ligase activity; chromatin binding; ATP binding; ligase activity

Biological Process: protein monoubiquitination; protein autoubiquitination; DNA repair; response to DNA damage stimulus

Disease: Fanconi Anemia, Complementation Group T