Western Blot (WB)
(Western blot analysis of UBE2T (arrow) using rabbit polyclonal UBE2T Antibody (C-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the UBE2T gene.)
Rabbit UBE2T Polyclonal Antibody | anti-UBE2T antibody
UBE2T Antibody (C-term)
Western Blot (WB)
(Western blot analysis of UBE2T (arrow) using rabbit polyclonal UBE2T Antibody (C-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the UBE2T gene.)
Western Blot (WB)
(Western blot analysis of UBE2T (arrow) using rabbit polyclonal UBE2T Antibody (C-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the UBE2T gene.)
regulates diverse cellular pathways and proteins. Ubiquitin is
transferred to a target protein through a concerted action of a
ubiquitin-activating enzyme (E1), a ubiquitin-conjugating enzyme
(E2), such as UBE2T, and a ubiquitin ligase (E3) (Machida et al.,
2006 [PubMed 16916645]).
Longerich, S., et al. J. Biol. Chem. 284(35):23182-23186(2009)
Alpi, A.F., et al. Mol. Cell 32(6):767-777(2008)
Hao, J., et al. Tumour Biol. 29(3):195-203(2008)
Machida, Y.J., et al. Mol. Cell 23(4):589-596(2006)
NCBI and Uniprot Product Information
NCBI Description
The covalent conjugation of ubiquitin to proteins regulates diverse cellular pathways and proteins. Ubiquitin is transferred to a target protein through a concerted action of a ubiquitin-activating enzyme (E1), a ubiquitin-conjugating enzyme (E2), such as UBE2T, and a ubiquitin ligase (E3) (Machida et al., 2006 [PubMed 16916645]).[supplied by OMIM, Mar 2008]
Uniprot Description
UBE2T: Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. Catalyzes monoubiquitination. Involved in mitomycin-C (MMC)-induced DNA repair: acts as a specific E2 ubiquitin-conjugating enzyme for the Fanconi anemia complex by associating with E3 ubiquitin-protein ligase FANCL and catalyzing monoubiquitination of FANCD2, a key step in the DNA damage pathway. Also mediates monoubiquitination of FANCL and FANCI. May contribute to ubiquitination and degradation of BRCA1. In vitro able to promote polyubiquitination using all 7 ubiquitin Lys residues, but may prefer 'Lys-11'-, 'Lys-27'-, 'Lys-48'- and 'Lys-63'-linked polyubiquitination. Belongs to the ubiquitin-conjugating enzyme family.
Protein type: Ubiquitin conjugating system; Ligase; Ubiquitin ligase; EC 6.3.2.19
Chromosomal Location of Human Ortholog: 1q32.1
Cellular Component: nucleoplasm; cytoplasm; nucleus
Molecular Function: small conjugating protein ligase activity; ubiquitin protein ligase binding; ubiquitin-protein ligase activity; chromatin binding; ATP binding; ligase activity
Biological Process: protein monoubiquitination; protein autoubiquitination; DNA repair; response to DNA damage stimulus
Disease: Fanconi Anemia, Complementation Group T
Research Articles on UBE2T
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Product Notes
The UBE2T ube2t (Catalog #AAA9209373) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 126-154. The UBE2T Antibody (C-term) reacts with Human (Predicted Reactivity: Bovine) and may cross-react with other species as described in the data sheet. AAA Biotech's UBE2T can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). WB~~1:1000. Researchers should empirically determine the suitability of the UBE2T ube2t for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "UBE2T, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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