SDS-PAGE
UBE2O recombinant protein
UBE2O recombinant protein
SDS-PAGE
SDS-PAGE
Scientific Background: UBE2O or ubiquitin-conjugating enzyme E2O is a part of the ubiquitin-mediated protein degradation pathway in which a thiol-ester linkage forms between a conserved cysteine and the C-terminus of ubiquitin and complexes with ubiquitin. HIV-1 Tat has been identified to have a physical interaction with ubiquitin-conjugating enzyme E2O (UBE2O) in human HEK293 and Jurkat cell lines by using affinity tagging and purification mass spectrometry analyses (1).
NCBI and Uniprot Product Information
Uniprot Description
Function: Catalyzes the covalent attachment of ubiquitin to other proteins
By similarity.
Catalytic activity: ATP + ubiquitin + protein lysine = AMP + diphosphate + protein N-ubiquityllysine.
Pathway: Protein modification; protein ubiquitination.
Tissue specificity: Predominantly expressed in skeletal muscle and heart. Ref.7
Sequence similarities: Belongs to the ubiquitin-conjugating enzyme family.
Sequence caution: The sequence AAH22237.1 differs from that shown. Reason: Erroneous initiation. The sequence AAH25977.1 differs from that shown. Reason: Erroneous initiation. The sequence AAH36820.1 differs from that shown. Reason: Erroneous initiation. The sequence AAH51868.2 differs from that shown. Reason: Erroneous initiation. The sequence BAB14320.1 differs from that shown. Reason: Erroneous initiation. The sequence BAB14948.1 differs from that shown. Reason: Erroneous initiation. The sequence BAB15313.1 differs from that shown. Reason: Erroneous initiation. The sequence BAB21825.1 differs from that shown. Reason: Erroneous initiation.
Research Articles on UBE2O
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Product Notes
The UBE2O ube2o (Catalog #AAA515416) is a Recombinant Protein produced from Sf9 insect cells and is intended for research purposes only. The product is available for immediate purchase. The tag for this protein is GST tag!!Expression System||Sf9 insect cells using baculovirus!!Source Note||Recombinant full-length human UBE2O was expressed by baculovirus in Sf9 insect cells. AAA Biotech's UBE2O can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Researchers should empirically determine the suitability of the UBE2O ube2o for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "UBE2O, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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