uBE2M recombinant protein

uBE2M, 1-183aa, Human, His tag, E Coli

Cat. Num. AAA204734

• Gene Names: UBE2M; UBC12; hUbc12; UBC-RS2
• Applications: SDS-Page
• Purity: > 95% by SDS-PAGE
• Synonyms: uBE2M; 1-183aa; Human; His tag; E Coli; NEDD8-conjugating enzyme ubc12; hubc12; uBC-RS2; uBC12; uBE2M recombinant protein

• Host: E Coli
• Purity/Purification: > 95% by SDS-PAGE
• Form/Format: Liquid. 20mM Tris-HCl Buffer (pH 8.0) containing 20% glycerol, 0.2M NaCl, 1mM DTT
• Concentration: 1 mg/ml (determined by Bradford assay) (varies by lot)
• Sequence: MGSSHHHHHH SSGLVPRGSH MGSHMIKLFS LKQQKKEEES AGGTKGSSKK ASAAQLRIQK DINELNLPKT CDISFSDPDD LLNFKLVICP DEGFYKSGKF VFSFKVGQGY PHDPPKVKCE TMVYHPNIDL EGNVCLNILR EDWKPVLTIN SIIYGLQYLF LEPNPEDPLN KEAAEVLQNN RRLFEQNVQR SMRGGYIGST YFERCLK
• Sequence Length: 183

• Applicable Applications for uBE2M recombinant protein: SDS-PAGE
• Antigen Species: Human
• Tag Information: His-tag
• Preparation and Storage: Can be stored at 4 degree C short term (1-2 weeks).; For long term storage, aliquot and store at -20 degree C or -70 degree C.; Avoid repeated freezing and thawing cycles.

SDS-Page

Related Product Information for uBE2M recombinant protein

uBE2M, also known as NEDD8-conjugating enzyme ubc12, belongs to the ubiquitin-conjugating enzyme family. The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. The encoded protein is linked with a ubiquitin-like protein, NEDD8, which can be conjugated to cellular proteins, such as Cdc53/culin. Recombinant human uBE2M protein, fused to His-tag at N-terminus, was expressed in E Coli and purified by using conventional chromatography.

Product Categories/Family for uBE2M recombinant protein

Ubiquitination

References

Gong L., et al. (1999) J. Biol. Chem. 274:12036-12042; Huang D.T., et al. (2004) Nat. Struct. Mol. Biol. 11:927-935

NCBI and Uniprot Product Information

• NCBI GI #: 4507791
• NCBI GeneID: 9040
• NCBI Accession #: NP_003960
• NCBI GenBank Nucleotide #: NM_003969.3
• UniProt Accession #: P61081; O76069; Q8VC50
• Molecular Weight: 23.5 kDa (207aa) confirmed by MALDI-TOF
• NCBI Official Full Name: NEDD8-conjugating enzyme Ubc12
• NCBI Official Synonym Full Names: ubiquitin conjugating enzyme E2 M
• NCBI Official Symbol: UBE2M
• NCBI Official Synonym Symbols: UBC12; hUbc12; UBC-RS2
• NCBI Protein Information: NEDD8-conjugating enzyme Ubc12
• UniProt Protein Name: NEDD8-conjugating enzyme Ubc12
• UniProt Gene Name: UBE2M
• UniProt Synonym Gene Names: UBC12

NCBI Description

The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. The encoded protein is linked with a ubiquitin-like protein, NEDD8, which can be conjugated to cellular proteins, such as Cdc53/culin. [provided by RefSeq, Jul 2008]

Uniprot Description

Accepts the ubiquitin-like protein NEDD8 from the UBA3-NAE1 E1 complex and catalyzes its covalent attachment to other proteins. The specific interaction with the E3 ubiquitin ligase RBX1, but not RBX2, suggests that the RBX1-UBE2M complex neddylates specific target proteins, such as CUL1, CUL2, CUL3 and CUL4. Involved in cell proliferation.