Western Blot (WB)
(Western blot analysis of lysates from human heart tissue lysate, U-251 MG cell line (from left to right), using AKTIP Antibody (C-term). MBS9201356 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35ug per lane.)
Rabbit AKTIP Polyclonal Antibody | anti-AKTIP antibody
AKTIP Antibody (C-term)
Western Blot (WB)
(Western blot analysis of lysates from human heart tissue lysate, U-251 MG cell line (from left to right), using AKTIP Antibody (C-term). MBS9201356 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35ug per lane.)
Western Blot (WB)
(Western blot analysis of lysates from human heart tissue lysate, U-251 MG cell line (from left to right), using AKTIP Antibody (C-term). MBS9201356 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35ug per lane.)
thymic hyperplasia in heterozygous mutant animals while homozygous
mutants die in early development. This gene may play a role in
apoptosis as these morphological abnormalities are caused by
altered patterns of programmed cell death. The protein encoded by
this gene is similar to the ubiquitin ligase domain of other
ubiquitin-conjugating enzymes but lacks the conserved cysteine
residue that enables those enzymes to conjugate ubiquitin to the
target protein. This protein interacts directly with
serine/threonine kinase protein kinase B (PKB)/Akt and modulates
PKB activity by enhancing the phosphorylation of PKB's regulatory
sites. Alternative splicing results in two transcript variants
encoding the same protein.
Quaye, L., et al. Hum. Mol. Genet. 18(10):1869-1878(2009)
Xu, L., et al. Mol. Biol. Cell 19(12):5059-5071(2008)
Lamesch, P., et al. Genomics 89(3):307-315(2007)
Ewing, R.M., et al. Mol. Syst. Biol. 3, 89 (2007) :
NCBI and Uniprot Product Information
NCBI Description
The mouse homolog of this gene produces fused toes and thymic hyperplasia in heterozygous mutant animals while homozygous mutants die in early development. This gene may play a role in apoptosis as these morphological abnormalities are caused by altered patterns of programmed cell death. The protein encoded by this gene is similar to the ubiquitin ligase domain of other ubiquitin-conjugating enzymes but lacks the conserved cysteine residue that enables those enzymes to conjugate ubiquitin to the target protein. This protein interacts directly with serine/threonine kinase protein kinase B (PKB)/Akt and modulates PKB activity by enhancing the phosphorylation of PKB's regulatory sites. Alternative splicing results in two transcript variants encoding the same protein. [provided by RefSeq, Jul 2008]
Uniprot Description
AKTIP: an oncogenic membrane-associated protein that is a component of the FTS/Hook/FHIP complex (FHF complex). The FHF complex may function to promote vesicle trafficking and/or fusion via the homotypic vesicular protein sorting complex (the HOPS complex). Regulates apoptosis by enhancing phosphorylation and activation of AKT1. Increases release of TNFSF6 via the AKT1/GSK3B/NFATC1 signaling cascade. Belongs to the ubiquitin-conjugating enzyme family. FTS subfamily. Component of the FTS/Hook/FHIP complex (FHF complex), composed of AKTIP/FTS, FAM160A2, and one or more members of the Hook family of proteins HOOK1, HOOK2, and HOOK3. May interact directly with HOOK1, HOOK2 and HOOK3. The FHF complex associates with the homotypic vesicular sorting complex (the HOPS complex). Apparently interacts with AKT1. A member of the ubiquitin-conjugating enzyme family, FTS subfamily. Lacks the conserved Cys residue necessary for ubiquitin-conjugating enzyme E2 activity. Two isoforms of the human protein are produced by alternative splicing.
Protein type: Vesicle; Membrane protein, peripheral; Apoptosis
Chromosomal Location of Human Ortholog: 16q12.2
Cellular Component: Golgi apparatus; cytoplasm; nucleolus; plasma membrane
Molecular Function: protein binding; small conjugating protein ligase activity; ubiquitin protein ligase binding
Biological Process: protein transport; endosome organization and biogenesis; positive regulation of protein binding; early endosome to late endosome transport; lysosome organization and biogenesis; apoptosis; endosome to lysosome transport; protein ubiquitination; positive regulation of protein amino acid phosphorylation
Research Articles on AKTIP
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Product Notes
The AKTIP aktip (Catalog #AAA9201356) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 262-288. The AKTIP Antibody (C-term) reacts with Human (Predicted Reactivity: Chicken, Monkey, Mouse, Rat) and may cross-react with other species as described in the data sheet. AAA Biotech's AKTIP can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). WB~~1:1000. Researchers should empirically determine the suitability of the AKTIP aktip for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "AKTIP, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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