Immunohistochemistry (IHC)
(Sample Type: Mouse BrainSample Type:NT2 cells Red:Antibody Blue:DAPI Primary Dilution:1ug/50ul antibody Secondary Antibody:Alexa goat anti-rabbit 594 Image Submitted by:Yuzhi Chen, University of Arkansas for Medical Sciences )
Rabbit UBE2M Polyclonal Antibody | anti-UBE2M antibody
UBE2M antibody - N-terminal region
Immunohistochemistry (IHC)
(Sample Type: Mouse BrainSample Type:NT2 cells Red:Antibody Blue:DAPI Primary Dilution:1ug/50ul antibody Secondary Antibody:Alexa goat anti-rabbit 594 Image Submitted by:Yuzhi Chen, University of Arkansas for Medical Sciences )
Immunohistochemistry (IHC)
(Sample Type: Mouse BrainSample Type:NT2 cells Red:Antibody Blue:DAPI Primary Dilution:1ug/50ul antibody Secondary Antibody:Alexa goat anti-rabbit 594 Image Submitted by:Yuzhi Chen, University of Arkansas for Medical Sciences )
Immunohistochemistry (IHC)
(Sample Type :Human brain stem cellsPrimary Antibody Dilution :1:500Secondary Antibody :Goat anti-rabbit Alexa-Fluor 594Secondary Antibody Dilution :1:1000Color/Signal Descriptions :UBE2M: Red DAPI:BlueGene Name :UBE2M Submitted by :Dr. Yuzhi Chen, University of Arkansas for Medical Science)
Immunohistochemistry (IHC)
(Sample Type :Human brain stem cellsPrimary Antibody Dilution :1:500Secondary Antibody :Goat anti-rabbit Alexa-Fluor 594Secondary Antibody Dilution :1:1000Color/Signal Descriptions :UBE2M: Red DAPI:BlueGene Name :UBE2M Submitted by :Dr. Yuzhi Chen, University of Arkansas for Medical Science)
Western Blot (WB)
(Sample Type: Mouse BrainSample Type:2. mouse brain extracts (80ug) Primary Antibody Dilution:2ug/ml Secondary Antibody: IRDye 800CW goat anti-rabbit from Li-COR Bioscience Secondary Antibody Dilution:1: 20,000 Image Submitted by: Yuzhi Chen University of Arkansas for Medical Science)
Western Blot (WB)
(Sample Type: Mouse BrainSample Type:2. mouse brain extracts (80ug) Primary Antibody Dilution:2ug/ml Secondary Antibody: IRDye 800CW goat anti-rabbit from Li-COR Bioscience Secondary Antibody Dilution:1: 20,000 Image Submitted by: Yuzhi Chen University of Arkansas for Medical Science)
Western Blot (WB)
(Host: RabbitTarget Name: UBE2MSample Type: Human 721_BAntibody Dilution: 1.0ug/mlUBE2M is supported by BioGPS gene expression data to be expressed in 721_B)
Western Blot (WB)
(Host: RabbitTarget Name: UBE2MSample Type: Human 721_BAntibody Dilution: 1.0ug/mlUBE2M is supported by BioGPS gene expression data to be expressed in 721_B)
Western Blot (WB)
(Host: RabbitTarget Name: UBE2MSample Type: Human MCF7Antibody Dilution: 1.0ug/mlUBE2M is supported by BioGPS gene expression data to be expressed in MCF7)
Western Blot (WB)
(Host: RabbitTarget Name: UBE2MSample Type: Human MCF7Antibody Dilution: 1.0ug/mlUBE2M is supported by BioGPS gene expression data to be expressed in MCF7)
Western Blot (WB)
(WB Suggested Anti-UBE2M Antibody Titration: 0.2-1 ug/mlPositive Control: 721_B cell lysateUBE2M is supported by BioGPS gene expression data to be expressed in 721_B)
Western Blot (WB)
(WB Suggested Anti-UBE2M Antibody Titration: 0.2-1 ug/mlPositive Control: 721_B cell lysateUBE2M is supported by BioGPS gene expression data to be expressed in 721_B)
Target Description: The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. UBE2M is a member of the E2 ubiquitin-conjugating enzyme family. It is linked with a ubiquitin-like protein, NEDD8, which can be conjugated to cellular proteins, such as Cdc53/culin.The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. The encoded protein is linked with a ubiquitin-like protein, NEDD8, which can be conjugated to cellular proteins, such as Cdc53/culin.
NCBI and Uniprot Product Information
NCBI Description
The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. The encoded protein is linked with a ubiquitin-like protein, NEDD8, which can be conjugated to cellular proteins, such as Cdc53/culin. [provided by RefSeq, Jul 2008]
Uniprot Description
UBE2M: Accepts the ubiquitin-like protein NEDD8 from the UBA3- NAE1 E1 complex and catalyzes its covalent attachment to other proteins. The specific interaction with the E3 ubiquitin ligase RBX1, but not RBX2, suggests that the RBX1-UBE2M complex neddylates specific target proteins, such as CUL1, CUL2, CUL3 and CUL4. Involved in cell proliferation. Interacts with UBA3, DCUN1D1 and RBX1. Belongs to the ubiquitin-conjugating enzyme family. UBC12 subfamily.
Protein type: Ubiquitin ligase; Ligase; Adaptor/scaffold; Ubiquitin conjugating system; EC 6.3.2.19; EC 6.3.2.-
Chromosomal Location of Human Ortholog: 19q13.43
Cellular Component: cytoplasm; cytosol
Molecular Function: protein binding; ubiquitin protein ligase binding; ubiquitin-protein ligase activity; ribosomal S6-glutamic acid ligase activity; ATP binding; NEDD8 ligase activity
Biological Process: protein neddylation; positive regulation of neuron apoptosis; transforming growth factor beta receptor signaling pathway; protein modification process; protein ubiquitination
Research Articles on UBE2M
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Product Notes
The UBE2M ube2m (Catalog #AAA3206671) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The UBE2M antibody - N-terminal region reacts with Cow, Dog, Guinea Pig, Horse, Human, Mouse, Rabbit, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's UBE2M can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC), Western Blot (WB). Researchers should empirically determine the suitability of the UBE2M ube2m for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Synthetic peptide located within the following region: IKLFSLKQQK KEEESAGGTK GSSKKASAAQ LRIQKDINEL NLPKTCDISF. It is sometimes possible for the material contained within the vial of "UBE2M, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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