Rabbit UBE2M Polyclonal Antibody | anti-UBE2M antibody

Anti-UBE2M Antibody, Rabbit Polyclonal

Cat. Num. AAA8100884

• Gene Names: UBE2M; UBC12; hUbc12; UBC-RS2
• Reactivity: Human; Predicted to React with: Mouse (Species predicted to react based on 100% sequence homology)
• Applications: Western Blot, Immunoprecipitation
• Purity: Protein A & Antigen Affinity
• Synonyms: UBE2M; Polyclonal Antibody; Anti-UBE2M Antibody; Rabbit Polyclonal; UBE2M Antibody; Rabbit PAb; Antigen Affinity Purified; ubiquitin-conjugating enzyme E2M; Anti-hUbc12 Antibody; Anti-UBC-RS2 Antibody; Anti-UBC12 Antibody; anti-UBE2M antibody

• Host: Rabbit
• Reactivity: Human; Predicted to React with: Mouse (Species predicted to react based on 100% sequence homology)
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Human UBE2M
• Purity/Purification: Protein A & Antigen Affinity
• Form/Format: Liquid; 0.2um filtered solution in PBS

• Applicable Applications for anti-UBE2M antibody: Western Blot (WB), Immunoprecipitation (IP)
• Application Notes: WB: 1:500-1:1000; IP: 0.2-1uL/mg of lysate
• Immunogen: A synthetic peptide corresponding to the C-terminus of the Human UBE2M
• Conjugation: Unconjugated
• Preparation: Produced in rabbits immunized with a synthetic peptide corresponding to the C-terminus of the Human UBE2M, and purified by antigen affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.

Immunoprecipitation (IP)

(UBE2M was immunoprecipitated using:Lane A:0.5 mg Jurkat Whole Cell Lysate0.5 uL anti-UBE2M rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein G.Primary antibody:Anti-UBE2M rabbit polyclonal antibody,at 1:500 dilution Secondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the odssey technique.Performed under reducing conditions.Predicted band size: 21 kDaObserved band size: 21 kDa)

Western Blot (WB)

(Anti-UBE2M rabbit polyclonal antibody at 1:500 dilutionLane A: Jurkat Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:21 kDaObserved band size:21 kDa(We are unsure as to the identity of these extra bands.))

Related Product Information for anti-UBE2M antibody

UBE2M is a member of the ubiquitin-conjugating E2 family whose members perform the second step in the ubiquitination reaction. Initially identified as the main process for protein degradation, ubiquitination is believed nowadays to be crucial for a wider range of cellular processes. The outcome of the ubiquitin-conjugation reaction, and thereby the fate of the substrate, is heavily dependent on the number of ubiquitin molecules attached and how these ubiquitin molecules are inter-connected. To deal with this complexity and to allow adequate ubiquitination in time and space, a highly sophisticated conjugation machinery has been developed. In a sequential manner, ubiquitin becomes activated by an ubiquitin-activating enzyme (E1), which then transfers the ubiquitin to a group of ubiquitin-conjugating enzymes (E2s). Next, ubiquitin-loaded E2s are interacting with ubiquitin-protein ligases (E3s) and ubiquitin is conjugated to substrates on recruitment by the E3. These three key enzymes are operating in a hierarchical system, wherein two E1s and 35 E2s have been found and hundreds of E3s have been identified in humans.

References

Sjoerd J L van Wijk, et al. (2009) A comprehensive framework of E2-RING E3 interactions of the human ubiquitin-proteasome system. Mol Syst Biol. 5: 317.Nandi D, et al. (2006) The ubiquitin-proteasome system. Journal of biosciences. 31 (1): 137-55.

NCBI and Uniprot Product Information

• NCBI GI #: 4507791
• NCBI GeneID: 9040
• NCBI Accession #: NP_003960.1
• NCBI GenBank Nucleotide #: NM_003969.3
• UniProt Accession #: P61081; O76069; Q8VC50
• Molecular Weight: 20,900 Da
• NCBI Official Full Name: NEDD8-conjugating enzyme Ubc12
• NCBI Official Synonym Full Names: ubiquitin-conjugating enzyme E2M
• NCBI Official Symbol: UBE2M
• NCBI Official Synonym Symbols: UBC12; hUbc12; UBC-RS2
• NCBI Protein Information: NEDD8-conjugating enzyme Ubc12; NEDD8 carrier protein; NEDD8 protein ligase; ubiquitin carrier protein M; ubiquitin-conjugating enzyme E2M (UBC12 homolog, yeast); ubiquitin-protein ligase M; yeast UBC12 homolog
• UniProt Protein Name: NEDD8-conjugating enzyme Ubc12
• UniProt Gene Name: UBE2M
• UniProt Synonym Gene Names: UBC12
• UniProt Entry Name: UBC12_HUMAN

NCBI Description

The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. The encoded protein is linked with a ubiquitin-like protein, NEDD8, which can be conjugated to cellular proteins, such as Cdc53/culin. [provided by RefSeq, Jul 2008]

Uniprot Description

UBE2M: Accepts the ubiquitin-like protein NEDD8 from the UBA3- NAE1 E1 complex and catalyzes its covalent attachment to other proteins. The specific interaction with the E3 ubiquitin ligase RBX1, but not RBX2, suggests that the RBX1-UBE2M complex neddylates specific target proteins, such as CUL1, CUL2, CUL3 and CUL4. Involved in cell proliferation. Interacts with UBA3, DCUN1D1 and RBX1. Belongs to the ubiquitin-conjugating enzyme family. UBC12 subfamily.

Protein type: Ubiquitin ligase; Ligase; Adaptor/scaffold; Ubiquitin conjugating system; EC 6.3.2.19; EC 6.3.2.-

Chromosomal Location of Human Ortholog: 19q13.43

Cellular Component: cytoplasm; cytosol

Molecular Function: protein binding; ubiquitin protein ligase binding; ubiquitin-protein ligase activity; ribosomal S6-glutamic acid ligase activity; ATP binding; NEDD8 ligase activity

Biological Process: protein neddylation; positive regulation of neuron apoptosis; transforming growth factor beta receptor signaling pathway; protein modification process; protein ubiquitination