Human C-C chemokine receptor type 7 ELISA Kit | CCR7 elisa kit

Human C-C chemokine receptor type 7 ELISA Kit

Cat. Num. AAA761979

• Gene Names: CCR7; BLR2; EBI1; CCR-7; CD197; CDw197; CMKBR7; CC-CKR-7
• Reactivity: Human
• Synonyms: C-C chemokine receptor type 7; Human C-C chemokine receptor type 7 ELISA Kit; CCR7/CD197/BLR2/C-C chemokine receptor type 7/C-C CKR-7/CC-CKR-7/CCR-7/CD197/CD197 antigen/CDw197/CC chemokine receptor 7/chemokine (C-C motif) receptor 7/CMKBR7/chemokine (C-C) receptor 7/EBI1/EBV-induced G protein-coupled receptor 1/EBV-induced G-protein coupled receptor 1/Epstein-Barr virus induced gene 1/Epstein-Barr virus induced G-protein coupled receptor/Epstein-Barr virus-induced G-protein coupled receptor 1/EVI1/lymphocyte-specific G protein-coupled peptide receptor/MIP-3 beta receptor; CCR7 elisa kit

• Reactivity: Human
• Sequence Length: 378

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Sandwich ELISA, Double Antibody
• Detection Range: 0.156-10ng/ml
• Sensitivity: 0.094ng/ml
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data

Typical Standard Curve

Related Product Information for CCR7 elisa kit

Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-Human CCR7 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Human CCR7 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human CCR7 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Human CCR7 can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 4502641
• NCBI GeneID: 1236
• NCBI Accession #: NP_001829.1
• NCBI GenBank Nucleotide #: NM_001838.3
• Molecular Weight: 42,874 Da
• NCBI Official Full Name: C-C chemokine receptor type 7
• NCBI Official Synonym Full Names: chemokine (C-C motif) receptor 7
• NCBI Official Symbol: CCR7
• NCBI Official Synonym Symbols: BLR2; EBI1; CCR-7; CD197; CDw197; CMKBR7; CC-CKR-7
• NCBI Protein Information: C-C chemokine receptor type 7
• UniProt Protein Name: C-C chemokine receptor type 7
• Protein Family: C-C chemokine receptor
• UniProt Gene Name: CCR7
• UniProt Synonym Gene Names: CMKBR7; EBI1; EVI1; C-C CKR-7; CC-CKR-7; CCR-7; EBI1; EBV-induced G-protein coupled receptor 1
• UniProt Entry Name: CCR7_HUMAN

NCBI Description

The protein encoded by this gene is a member of the G protein-coupled receptor family. This receptor was identified as a gene induced by the Epstein-Barr virus (EBV), and is thought to be a mediator of EBV effects on B lymphocytes. This receptor is expressed in various lymphoid tissues and activates B and T lymphocytes. It has been shown to control the migration of memory T cells to inflamed tissues, as well as stimulate dendritic cell maturation. The chemokine (C-C motif) ligand 19 (CCL19/ECL) has been reported to be a specific ligand of this receptor. Signals mediated by this receptor regulate T cell homeostasis in lymph nodes, and may also function in the activation and polarization of T cells, and in chronic inflammation pathogenesis. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, Sep 2014]

Uniprot Description

CCR7: Receptor for the MIP-3-beta chemokine. Probable mediator of EBV effects on B-lymphocytes or of normal lymphocyte functions. Belongs to the G-protein coupled receptor 1 family.

Protein type: Motility/polarity/chemotaxis; GPCR, family 1; Receptor, GPCR; Membrane protein, integral; Membrane protein, multi-pass

Chromosomal Location of Human Ortholog: 17q12-q21.2

Cellular Component: cell surface; cytosol; external side of plasma membrane; integral to membrane; intracellular; plasma membrane

Molecular Function: C-C chemokine receptor activity; G-protein coupled receptor activity

Biological Process: dendritic cell chemotaxis; elevation of cytosolic calcium ion concentration; establishment of T cell polarity; G-protein coupled receptor protein signaling pathway; homeostasis of number of cells; immune response; inflammatory response; myeloid dendritic cell chemotaxis; negative thymic T cell selection; positive regulation of actin filament polymerization; positive regulation of cell adhesion; positive regulation of cell-matrix adhesion; positive regulation of dendritic cell antigen processing and presentation; positive regulation of filopodium formation; positive regulation of humoral immune response; positive regulation of hypersensitivity; positive regulation of I-kappaB kinase/NF-kappaB cascade; positive regulation of interleukin-12 production; positive regulation of JNK cascade; positive regulation of phosphoinositide 3-kinase activity; positive regulation of protein kinase activity; positive regulation of protein kinase B signaling cascade; positive regulation of pseudopodium formation; positive regulation of T cell receptor signaling pathway; regulation of interferon-gamma production; regulation of interleukin-1 beta secretion; release of sequestered calcium ion into cytosol; response to lipopolysaccharide; ruffle organization and biogenesis