ubc9 (Human ubiquitin conjugating enzyme 9) Recombinant Protein | ubc9 recombinant protein

ubc9 (Human ubiquitin conjugating enzyme 9), 1-158 aa, Human, E Coli

Cat. Num. AAA203069

• Gene Names: UBE2I; P18; UBC9; C358B7.1
• Applications: SDS-Page
• Purity: > 95% by SDS-PAGE
• Synonyms: ubc9 (Human ubiquitin conjugating enzyme 9); 1-158 aa; Human; E Coli; ubiquitin-conjugating enzyme E2I; uBE2I; uBC9; uBCE9; ubc9; Human ubiquitin conjugating enzyme 9; EC 6.3.2; SuMO-protein ligase; ubiquitin-conjugating enzyme E2 I; ubiquitin-protein ligase I; ubiquitin carrier protein I; ubiquitin carrier protein 9; p18; SuMO-conjugating enzyme uBC9; ubiquitin-conjugating enzyme E2I C358B7.1; Homologous to yeast uBC9; SuMO 1 conjugating enzyme; SuMO 1 protein ligase; SuMO conjugating enzyme uBC9; SuMO protein ligase; SuMO1 conjugating enzyme; SuMO1 protein ligase; uBC 9; uBC9 homolog; uBCE 9; uBE 2I; ubiquitin carrier protein; ubiquitin conjugating enzyme 9; ubiquitin conjugating enzyme E2I; ubiquitin conjugating enzyme ubcE2A; ubiquitin like protein SuMO 1 conjugating enzyme; ubiquitin like protein SuMO1 conjugating enzyme; ubiquitin protein ligase E2I; ubiquitin protein ligase I; ; ubc9 recombinant protein

• Host: E Coli
• Purity/Purification: > 95% by SDS-PAGE
• Form/Format: Liquid. In 50 mM HEPES (pH 7.5) 150mM NaCl, 1mM DTT, 10% glycerol.
• Concentration: 1 mg/ml (determined by Bradford assay) (varies by lot)
• Sequence: MSGIALSRLA QERKAWRKDH PFGFVAVPTK NPDGTMNLMN WECAIPGKKG TPWEGGLFKL RMLFKDDYPS SPPKCKFEPP LFHPNVYPSG TVCLSILEED KDWRPAITIK QILLGIQELL NEPNIQDPAQ AEAYTIYCQN RVEYEKRVRA QAKKFAPS
• Sequence Length: 158

• Applicable Applications for ubc9 recombinant protein: SDS-PAGE
• Antigen Species: Human
• Endotoxin Level: < 1.0 EU per 1 microgram of protein (determined by LAL method)
• Preparation and Storage: Can be stored at 4 degree C short term (1-2 weeks).; For long term storage, aliquot and store at -20 degree C or -70 degree C.; Avoid repeated freezing and thawing cycles.

SDS-Page

Related Product Information for ubc9 recombinant protein

Human ubc9 is homologous to ubiquitin-conjugating enzymes (E2s). However, instead of conjugating ubiquitin, it conjugates a ubiquitin homologue, small ubiquitin-like modifier 1 (SuMO-1). And hubc9 retains striking structural and functional conservation with yeast ubc9. The ubiquitin-dependent protein degradation system has been recognized as a complete enzymatic pathway that is responsible for the selective degradation of abnormal and short-lived proteins. The conjugation of ubiquitin reuires the activities of ubiquitin-activating (E1) and -conjugating (E2) enzymes.

Product Categories/Family for ubc9 recombinant protein

Ubiquitination

References

Tatham MH., et al (2003) Biochemistry. 42 (11) 3168-3179; Donghai Lin, et al (2002) J. Biol. Chem. 277 (24) 21740-21748.; Wang ZY., et al. (1996) J. Biol. Chem. 271 (40):24811-6.;

NCBI and Uniprot Product Information

• NCBI GI #: 4507785
• NCBI GeneID: 7329
• NCBI Accession #: NP_003336
• NCBI GenBank Nucleotide #: NM_003345.4
• UniProt Accession #: P63279; P50550; Q15698; Q59GX1; Q86VB3; D3DU69
• Molecular Weight: 18 kDa (158 aa), confirmed by MALDI-TOF.
• NCBI Official Full Name: SUMO-conjugating enzyme UBC9
• NCBI Official Synonym Full Names: ubiquitin conjugating enzyme E2 I
• NCBI Official Symbol: UBE2I
• NCBI Official Synonym Symbols: P18; UBC9; C358B7.1
• NCBI Protein Information: SUMO-conjugating enzyme UBC9
• UniProt Protein Name: SUMO-conjugating enzyme UBC9
• UniProt Gene Name: UBE2I
• UniProt Synonym Gene Names: UBC9; UBCE9

NCBI Description

The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. Four alternatively spliced transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

Accepts the ubiquitin-like proteins SUMO1, SUMO2, SUMO3 and SUMO4 from the UBLE1A-UBLE1B E1 complex and catalyzes their covalent attachment to other proteins with the help of an E3 ligase such as RANBP2, CBX4 and ZNF451. Can catalyze the formation of poly-SUMO chains. Necessary for sumoylation of FOXL2 and KAT5. Essential for nuclear architecture and chromosome segregation. Sumoylates p53/TP53 at 'Lys-386'.