Rad6/UBE2A Recombinant Protein | UBE2A recombinant protein

Rad6/UBE2A Protein, Human, Recombinant (His Tag)

Cat. Num. AAA8121219

• Gene Names: UBE2A; UBC2; HHR6A; MRXSN; RAD6A; MRXS30
• Purity: >80% as determined by SDS-PAGE
• Synonyms: Rad6/UBE2A; Rad6/UBE2A Protein; Human; Recombinant (His Tag); Human UBE2A/HHR6A Protein (His Tag); HHR6A Protein; MRXS30 Protein; MRXSN Protein; RAD6A Protein; UBC2 Protein; ubiquitin-conjugating enzyme E2A; UBE2A recombinant protein

• Host: E Coli
• Purity/Purification: >80% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, 20% glycerol, pH7.5. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Met1-Cys152

• Species: Human
• Predicted N Terminal: Met 1
• Tag: N-His
• Protein Construction: A DNA sequence encoding the mature form of human UBE2A (P49459)(Met 1-Cys 152) was expressed, with a polyhistidine tag at the N-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for UBE2A recombinant protein

Background: Ubiquitin-conjugating enzyme E2 A (also known as HHR6A or UBE2A), encoded by human DNA repair genes HHR6A, belongs to the ubiquitin-conjugating enzymes (E2 enzymes) family and is likely to be involved in postreplication repair and induced mutagenesis. UBE2A is described as a CDK2 substrate. It is the human homologue of the product of the Saccharomyces cerevisiae RAD6/UBC2 gene, a member of the family of ubiquitin-conjugating enzymes. In vivo, HHR6A phosphorylation peaks during the G2/M phase of cell cycle transition, with a concomitant increase in histone H2B ubiquitylation. Mutation of Ser120 to threonine or alanine abolished UBE2A activity, while mutation to aspartate to mimic phosphorylated serine increased UBE2A activity 3-fold. A mutation of UBE2A is considered as the cause of a novel X-linked mental retardation (XLMR) syndrome that affects three males in a two-generation family.

References

Nascimento RM, et al. (2006) UBE2A, which encodes a ubiquitin-conjugating enzyme, is mutated in a novel X-linked mental retardation syndrome. Am J Hum Genet. 79 (3): 549-55.Koken MH, et al. (1992) Localization of two human homologs, HHR6A and HHR6B, of the yeast DNA repair gene RAD6 to chromosomes Xq24-q25 and 5q23-q31. Genomics. 12 (3): 447-53.Sarcevic B, et al. (2002) Regulation of the ubiquitin-conjugating enzyme hHR6A by CDK-mediated phosphorylation. EMBO J. 21 (8): 2009-18.

NCBI and Uniprot Product Information

• NCBI GI #: 33518639
• NCBI GeneID: 7319
• UniProt Accession #: P49459; Q4TTG1; Q96FX4; A6NFE9; A6NGR2; A6NMF5; B2R7R9; D3DWI1
• Molecular Weight: 152
• NCBI Official Full Name: Ubiquitin-conjugating enzyme E2 A
• NCBI Official Synonym Full Names: ubiquitin-conjugating enzyme E2A
• NCBI Official Symbol: UBE2A
• NCBI Official Synonym Symbols: UBC2; HHR6A; MRXSN; RAD6A; MRXS30
• NCBI Protein Information: ubiquitin-conjugating enzyme E2 A; RAD6 homolog A; ubiquitin-protein ligase A; ubiquitin carrier protein A
• UniProt Protein Name: Ubiquitin-conjugating enzyme E2 A
• Protein Family: Ubiquitin-conjugating enzyme
• UniProt Gene Name: UBE2A
• UniProt Synonym Gene Names: RAD6A; HR6A; hHR6A
• UniProt Entry Name: UBE2A_HUMAN

NCBI Description

The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, ubiquitin-conjugating enzymes, and ubiquitin-protein ligases. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. This enzyme is required for post-replicative DNA damage repair, and may play a role in transcriptional regulation. Mutations in this gene are associated with mental retardation. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2013]

Uniprot Description

UBE2A: a ubiquitin-protein ligase, a member of the ubiquitin-conjugating enzyme family. Homologous to the yeast DNA repair gene RAD6. Interacts with RAD18. Required for postreplication repair of UV-damaged DNA.

Protein type: EC 6.3.2.19; Ubiquitin conjugating system; Ubiquitin ligase; Ligase

Chromosomal Location of Human Ortholog: Xq24

Cellular Component: XY body; cytoplasm; nuclear chromatin; chromatin; cytosol

Molecular Function: protein binding; small conjugating protein ligase activity; ubiquitin protein ligase binding; ubiquitin-protein ligase activity; ATP binding; ligase activity

Biological Process: ubiquitin-dependent protein catabolic process; proteasomal ubiquitin-dependent protein catabolic process; antigen processing and presentation of peptide antigen via MHC class I; protein autoubiquitination; protein polyubiquitination; maternal process involved in pregnancy; postreplication repair; in utero embryonic development; positive regulation of cell proliferation; DNA repair; histone H2A ubiquitination; response to UV

Disease: Mental Retardation, X-linked, Syndromic, Nascimento Type