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SDS-PAGE

UBE2A / HHR6A recombinant protein

Recombinant Human UBE2A / HHR6A Protein (His tag)

Gene Names
UBE2A; UBC2; HHR6A; MRXSN; RAD6A; MRXS30
Purity
> 80 % as determined by SDS-PAGE
Synonyms
UBE2A / HHR6A; Recombinant Human UBE2A / HHR6A Protein (His tag); HHR6A; RAD6A; UBC2; UBE2A / HHR6A recombinant protein
Ordering
For Research Use Only!
Host
E Coli
Purity/Purification
> 80 % as determined by SDS-PAGE
Form/Format
Lyophilized from sterile PBS, 20% glycerol, pH 7.5
Sequence Length
119
Application Notes
The recombinant human UBE2A consisting of 167 amino acids and has a calculated molecular mass of 19.2 kDa. It migrates as an approximately 18.5 kDa band in SDS-PAGE under reducing conditions.
Predicted N Terminal
Met 1
Preparation and Storage
Samples are stable for up to twelve months from date of receipt at -70 degree C

SDS-PAGE

SDS-PAGE
Related Product Information for UBE2A / HHR6A recombinant protein
Background: Ubiquitin-conjugating enzyme E2 A (also known as HHR6A or UBE2A), encoded by human DNA repair genes HHR6A, belongs to the ubiquitin-conjugating enzymes (E2 enzymes) family and is likely to be involved in postreplication repair and induced mutagenesis. UBE2A is described as a CDK2 substrate. It is the human homologue of the product of the Saccharomyces cerevisiae RAD6 / UBC2 gene, a member of the family of ubiquitin-conjugating enzymes. In vivo, HHR6A phosphorylation peaks during the G2/M phase of cell cycle transition, with a concomitant increase in histone H2B ubiquitylation. Mutation of Ser120 to threonine or alanine abolished UBE2A activity, while mutation to aspartate to mimic phosphorylated serine increased UBE2A activity 3-fold. A mutation of UBE2A is consisdered as the cause of a novel X-linked mental retardation (XLMR) syndrome that affects three males in a two-generation family. 

Description: A DNA sequence encoding the mature form of human UBE2A (P49459) (Met 1-Cys 152) was expressed, with a polyhistidine tag at the N-terminus.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
8,833 Da
NCBI Official Full Name
ubiquitin-conjugating enzyme E2 A isoform 4
NCBI Official Synonym Full Names
ubiquitin conjugating enzyme E2 A
NCBI Official Symbol
UBE2A
NCBI Official Synonym Symbols
UBC2; HHR6A; MRXSN; RAD6A; MRXS30
NCBI Protein Information
ubiquitin-conjugating enzyme E2 A
UniProt Protein Name
Ubiquitin-conjugating enzyme E2 A
UniProt Gene Name
UBE2A
UniProt Synonym Gene Names
RAD6A; HR6A; hHR6A

NCBI Description

The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, ubiquitin-conjugating enzymes, and ubiquitin-protein ligases. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. This enzyme is required for post-replicative DNA damage repair, and may play a role in transcriptional regulation. Mutations in this gene are associated with mental retardation. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2013]

Uniprot Description

UBE2A: a ubiquitin-protein ligase, a member of the ubiquitin-conjugating enzyme family. Homologous to the yeast DNA repair gene RAD6. Interacts with RAD18. Required for postreplication repair of UV-damaged DNA.

Protein type: EC 6.3.2.19; Ligase; Ubiquitin conjugating system; Ubiquitin ligase

Chromosomal Location of Human Ortholog: Xq24

Cellular Component: chromatin; cytoplasm; cytosol; nuclear chromatin

Molecular Function: protein binding; ubiquitin binding; ubiquitin protein ligase binding; ubiquitin-protein ligase activity

Biological Process: DNA repair; histone H2A ubiquitination; positive regulation of cell proliferation; proteasomal ubiquitin-dependent protein catabolic process; protein autoubiquitination; protein polyubiquitination; response to UV

Disease: Mental Retardation, X-linked, Syndromic, Nascimento Type

Research Articles on UBE2A / HHR6A

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Product Notes

The UBE2A / HHR6A ube2a (Catalog #AAA2545458) is a Recombinant Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. The recombinant human UBE2A consisting of 167 amino acids and has a calculated molecular mass of 19.2 kDa. It migrates as an approximately 18.5 kDa band in SDS-PAGE under reducing conditions. Researchers should empirically determine the suitability of the UBE2A / HHR6A ube2a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "UBE2A / HHR6A, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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