Western Blot (WB)
(Anti-UBE2A rabbit polyclonal antibody at 1:500 dilution Lane A: HeLa Whole Cell Lysate Lane B: Jurkat Whole Cell Lysate Lane C: 293T Whole Cell Lysate Lane D: COLO205 Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:17 kDa Observed band size:17 kDa)
Rabbit anti-Human Rad6/UBE2A Polyclonal Antibody | anti-UBE2A antibody
Anti-Rad6/UBE2A Antibody, Rabbit Polyclonal
ELISA: 1:5000-1:10,000
ICC/IF: 1:200-1:1000
IP: 0.5-2uL/mg of lysate
Western Blot (WB)
(Anti-UBE2A rabbit polyclonal antibody at 1:500 dilution Lane A: HeLa Whole Cell Lysate Lane B: Jurkat Whole Cell Lysate Lane C: 293T Whole Cell Lysate Lane D: COLO205 Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:17 kDa Observed band size:17 kDa)
Western Blot (WB)
(Anti-UBE2A rabbit polyclonal antibody at 1:500 dilution Lane A: HeLa Whole Cell Lysate Lane B: Jurkat Whole Cell Lysate Lane C: 293T Whole Cell Lysate Lane D: COLO205 Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:17 kDa Observed band size:17 kDa)
Immunofluorescence (IF)
(Immunofluorescence staining of UBE2A in HeLa cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-Human UBE2A polyclonal antibody (dilution ratio 1:500) at 4 degree C overnight. Then cells were stained with the Alexa Fluor488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to Nucleus and cytoplasm.)
Immunofluorescence (IF)
(Immunofluorescence staining of UBE2A in HeLa cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-Human UBE2A polyclonal antibody (dilution ratio 1:500) at 4 degree C overnight. Then cells were stained with the Alexa Fluor488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to Nucleus and cytoplasm.)
Immunoprecipitation (IP)
(UBE2A was immunoprecipitated using: Lane A:0.5 mg HeLa Whole Cell Lysate 4 uL anti-UBE2A rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G. Primary antibody: Anti-UBE2A rabbit polyclonal antibody,at 1:100 dilution Secondary antibody: Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 17 kDa Observed band size :17 kDa)
Immunoprecipitation (IP)
(UBE2A was immunoprecipitated using: Lane A:0.5 mg HeLa Whole Cell Lysate 4 uL anti-UBE2A rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G. Primary antibody: Anti-UBE2A rabbit polyclonal antibody,at 1:100 dilution Secondary antibody: Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 17 kDa Observed band size :17 kDa)
NCBI and Uniprot Product Information
NCBI Description
The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, ubiquitin-conjugating enzymes, and ubiquitin-protein ligases. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. This enzyme is required for post-replicative DNA damage repair, and may play a role in transcriptional regulation. Mutations in this gene are associated with mental retardation. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2013]
Uniprot Description
UBE2A: a ubiquitin-protein ligase, a member of the ubiquitin-conjugating enzyme family. Homologous to the yeast DNA repair gene RAD6. Interacts with RAD18. Required for postreplication repair of UV-damaged DNA.
Protein type: EC 6.3.2.19; Ubiquitin conjugating system; Ubiquitin ligase; Ligase
Chromosomal Location of Human Ortholog: Xq24
Cellular Component: XY body; cytoplasm; nuclear chromatin; chromatin; cytosol
Molecular Function: protein binding; small conjugating protein ligase activity; ubiquitin protein ligase binding; ubiquitin-protein ligase activity; ATP binding; ligase activity
Biological Process: ubiquitin-dependent protein catabolic process; proteasomal ubiquitin-dependent protein catabolic process; antigen processing and presentation of peptide antigen via MHC class I; protein autoubiquitination; protein polyubiquitination; maternal process involved in pregnancy; postreplication repair; in utero embryonic development; positive regulation of cell proliferation; DNA repair; histone H2A ubiquitination; response to UV
Disease: Mental Retardation, X-linked, Syndromic, Nascimento Type
Research Articles on UBE2A
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Product Notes
The UBE2A ube2a (Catalog #AAA8106591) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-Rad6/UBE2A Antibody, Rabbit Polyclonal reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Rad6/UBE2A can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP). WB: 1:500-1:2000 ELISA: 1:5000-1:10,000 ICC/IF: 1:200-1:1000 IP: 0.5-2uL/mg of lysate. Researchers should empirically determine the suitability of the UBE2A ube2a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Rad6/UBE2A, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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