Ubiquitin-Conjugating Enzyme E2 A Recombinant Protein | UBE2A recombinant protein

Recombinant Human Ubiquitin-Conjugating Enzyme E2 A Protein

Cat. Num. AAA2556627

• Gene Names: UBE2A; UBC2; HHR6A; MRXSN; RAD6A; MRXS30
• Purity: >80% as determined by reducing SDS-PAGE.
• Synonyms: Ubiquitin-Conjugating Enzyme E2 A; Recombinant Human Ubiquitin-Conjugating Enzyme E2 A Protein; RAD6 Homolog A; HR6A; hHR6A; Ubiquitin Carrier Protein A; Ubiquitin-Protein Ligase A; UBE2A; RAD6A; UBE2A recombinant protein

• Host: E Coli
• Purity/Purification: >80% as determined by reducing SDS-PAGE.
• Form/Format: Supplied as a 0.2 mum filtered solution of 50mM HEPES, 150mM NaCl, 2mM DTT, 10% Glycerol, pH 7.5.
• Sequence Length: 119

• Species: Human
• Endotoxin: <1.0 EU per ug as determined by LAL test.
• Fragment: Met1-Cys152
• Fusion Tag: N-6His
• Preparation and Storage: Store at < -20 degree C, stable for 6 months after receipt.; Please minimize freeze-thaw cycles.

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Related Product Information for UBE2A recombinant protein

Protein Construction: Recombinant Human Ubiquitin-Conjugating Enzyme E2 A is produced by our E Coli expression system and the target gene encoding Met1-Cys152 is expressed with a GST, 6His tag at the N-terminus.

Background: Ubiquitin-Conjugating Enzyme E2 (UBE2A) is a member of the E2 Ubiquitin-Conjugating Enzyme family. The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. UBE2A catalyzes the covalent attachment of ubiquitin to other proteins. UBE2A is required for postreplication repair of UV-damaged DNA. UBE2A Interacts with RAD18 and WAC.

NCBI and Uniprot Product Information

• NCBI GI #: 531990811
• NCBI GeneID: 7319
• NCBI Accession #: NP_001269090.1
• NCBI GenBank Nucleotide #: NM_001282161.1
• UniProt Accession #: P49459; Q4TTG1; Q96FX4; A6NFE9; A6NGR2; A6NMF5; B2R7R9; D3DWI1
• Molecular Weight: Molecular Mass: 49.0kDa; Actual Protein Molecular Mass: 52kDa
• NCBI Official Full Name: ubiquitin-conjugating enzyme E2 A isoform 4
• NCBI Official Synonym Full Names: ubiquitin conjugating enzyme E2 A
• NCBI Official Symbol: UBE2A
• NCBI Official Synonym Symbols: UBC2; HHR6A; MRXSN; RAD6A; MRXS30
• NCBI Protein Information: ubiquitin-conjugating enzyme E2 A
• UniProt Protein Name: Ubiquitin-conjugating enzyme E2 A
• Protein Family: Ubiquitin-conjugating enzyme
• UniProt Gene Name: UBE2A
• UniProt Synonym Gene Names: RAD6A; HR6A; hHR6A

NCBI Description

The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, ubiquitin-conjugating enzymes, and ubiquitin-protein ligases. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. This enzyme is required for post-replicative DNA damage repair, and may play a role in transcriptional regulation. Mutations in this gene are associated with cognitive disability. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2013]

Uniprot Description

Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In association with the E3 enzyme BRE1 (RNF20 and/or RNF40), it plays a role in transcription regulation by catalyzing the monoubiquitination of histone H2B at 'Lys-120' to form H2BK120ub1. H2BK120ub1 gives a specific tag for epigenetic transcriptional activation, elongation by RNA polymerase II, telomeric silencing, and is also a prerequisite for H3K4me and H3K79me formation. In vitro catalyzes 'Lys-11', as well as 'Lys-48'-linked polyubiquitination. Required for postreplication repair of UV-damaged DNA.