SDS-Page
TYW5 recombinant protein
TYW5, 1-315aa, Human, His tag, E Coli
Gene Names
TYW5; hTYW5; C2orf60
Applications
SDS-Page
Purity
> 95% by SDS-PAGE
Synonyms
TYW5; 1-315aa; Human; His tag; E Coli; tRNA wybutosine-synthesizing protein 5; C2orf60; Htyw5; TYW5 recombinant protein
Host
E Coli
Purity/Purification
> 95% by SDS-PAGE
Form/Format
Liquid. In 20mM Tris-HCl Buffer (pH 8.0) containing 0.15M NaCl, 10% glycerol, 1mM DTT
Concentration
1 mg/ml (determined by Bradford assay) (varies by lot)
Sequence
MGSSHHHHHH SSGLVPRGSH MGSMAGQHLP VPRLEGVSRE QFMQHLYPQR KPLVLEGIDL GPCTSKWTVD YLSQVGGKKE VKIHVAAVAQ MDFISKNFVY RTLPFDQLVQ RAAEEKHKEF FVSEDEKYYL RSLGEDPRKD VADIRKQFPL LKGDIKFPEF FKEEQFFSSV FRISSPGLQL WTHYDVMDNL LIQVTGKKRV VLFSPRDAQY LYLKGTKSEV LNIDNPDLAK YPLFSKARRY ECSLEAGDVL FIPALWFHNV ISEEFGVGVN IFWKHLPSEC YDKTDTYGNK DPTAASRAAQ ILDRALKTLA ELPEEYRDFY ARRMVLHIQD KAYSKNSE
Sequence Length
315
Applicable Applications for TYW5 recombinant protein
SDS-PAGE
Antigen Species
Human
Tag Information
His-tag
Preparation and Storage
Can be stored at 4 degree C short term (1-2 weeks).
For long term storage, aliquot and store at -20 degree C or -70 degree C.
Avoid repeated freezing and thawing cycles.
For long term storage, aliquot and store at -20 degree C or -70 degree C.
Avoid repeated freezing and thawing cycles.
SDS-Page
SDS-Page
Related Product Information for TYW5 recombinant protein
tRNA wybutosine-synthesizing protein 5, also known as TYW5, acts as a component of the wybutosine biosynthesis pathway. Wybutosine is a hyper modified guanosine with a tricyclic base found at the 3'-position adjacent to the anticodon of eukaryotic phenylalanine tRNA. TYW5 catalyzes the hydroxylation of 7- (a-amino-a-carboxypropyl)wyosine (yW-72) into undermodified hydroxywybutosine (OHyW). OHyW is a derivative of wybutosine found in higher eukaryotes. Recombinant human TYW5 protein, fused to His-tag at N-terminus, was expressed in E Coli and purified by using conventional chromatography techniques.
Product Categories/Family for TYW5 recombinant protein
References
Noma A., et al. (2010) J. Biol. Chem. 285:34503-34507; Kato M., et al. (2011) Nucleic Acids Res. 39:1576-1585;
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
38.9kDa (338aa), confirmed by MALDI-TOF
NCBI Official Full Name
tRNA wybutosine-synthesizing protein 5
NCBI Official Synonym Full Names
tRNA-yW synthesizing protein 5
NCBI Official Symbol
TYW5
NCBI Official Synonym Symbols
hTYW5; C2orf60
NCBI Protein Information
tRNA wybutosine-synthesizing protein 5
UniProt Protein Name
tRNA wybutosine-synthesizing protein 5
Protein Family
UniProt Gene Name
TYW5
UniProt Synonym Gene Names
C2orf60; hTYW5
Uniprot Description
tRNA hydroxylase that acts as a component of the wybutosine biosynthesis pathway. Wybutosine is a hyper modified guanosine with a tricyclic base found at the 3'-position adjacent to the anticodon of eukaryotic phenylalanine tRNA. Catalyzes the hydroxylation of 7-(a-amino-a-carboxypropyl)wyosine (yW-72) into undermodified hydroxywybutosine (OHyW*). OHyW* being further transformed into hydroxywybutosine (OHyW) by LCMT2/TYW4. OHyW is a derivative of wybutosine found in higher eukaryotes.
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Product Notes
The TYW5 tyw5 (Catalog #AAA205166) is a Recombinant Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. The tag for this protein is His-tag. AAA Biotech's TYW5 can be used in a range of immunoassay formats including, but not limited to, SDS-PAGE. Researchers should empirically determine the suitability of the TYW5 tyw5 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: MGSSHHHHHH SSGLVPRGSH MGSMAGQHLP VPRLEGVSRE QFMQHLYPQR KPLVLEGIDL GPCTSKWTVD YLSQVGGKKE VKIHVAAVAQ MDFISKNFVY RTLPFDQLVQ RAAEEKHKEF FVSEDEKYYL RSLGEDPRKD VADIRKQFPL LKGDIKFPEF FKEEQFFSSV FRISSPGLQL WTHYDVMDNL LIQVTGKKRV VLFSPRDAQY LYLKGTKSEV LNIDNPDLAK YPLFSKARRY ECSLEAGDVL FIPALWFHNV ISEEFGVGVN IFWKHLPSEC YDKTDTYGNK DPTAASRAAQ ILDRALKTLA ELPEEYRDFY ARRMVLHIQD KAYSKNSE. It is sometimes possible for the material contained within the vial of "TYW5, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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