Typical Testing Data/Standard Curve (for reference only)
Mouse thioredoxin interacting protein ELISA Kit | TXNIP elisa kit
Mouse Thioredoxin-interacting protein, TXNIP ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for TXNIP has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any TXNIP present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TXNIP is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TXNIP bound in the initial step. The color development is stopped and the intensity of the color is measured.
NCBI and Uniprot Product Information
Uniprot Description
TXNIP: May act as an oxidative stress mediator by inhibiting thioredoxin activity or by limiting its bioavailability. Interacts with COPS5 and restores COPS5-induced suppression of CDKN1B stability, blocking the COPS5-mediated translocation of CDKN1B from the nucleus to the cytoplasm. Functions as a transcriptional repressor, possibly by acting as a bridge molecule between transcription factors and corepressor complexes, and over- expression will induce G0/G1 cell cycle arrest. Required for the maturation of natural killer cells. Acts as a suppressor of tumor cell growth. Belongs to the arrestin family.
Protein type: Apoptosis; Motility/polarity/chemotaxis; Tumor suppressor; Mitochondrial
Cellular Component: cell; cytoplasm; mitochondrial intermembrane space; intracellular; nucleus
Molecular Function: enzyme inhibitor activity; protein binding; ubiquitin protein ligase binding
Biological Process: keratinocyte differentiation; regulation of transcription, DNA-dependent; transcription, DNA-dependent; positive regulation of apoptosis; protein import into nucleus; negative regulation of catalytic activity; platelet-derived growth factor receptor signaling pathway; negative regulation of transcription from RNA polymerase II promoter; response to oxidative stress; negative regulation of cell division; cell cycle; regulation of cell proliferation
Research Articles on TXNIP
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Product Notes
The Mouse TXNIP txnip (Catalog #AAA942167) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA942167 ELISA Kit recognizes Mouse TXNIP. It is sometimes possible for the material contained within the vial of "thioredoxin interacting protein, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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