Human thioredoxin interacting protein ELISA Kit | TXNIP elisa kit

Human Thioredoxin-interacting protein, TXNIP ELISA Kit

Cat. Num. AAA907170

• Gene Names: TXNIP; THIF; VDUP1; HHCPA78; EST01027
• Reactivity: Human
• Synonyms: thioredoxin interacting protein; Human Thioredoxin-interacting protein; TXNIP ELISA Kit; Human Thioredoxin-interacting protein (TXNIP) ELISA kit; EST01027; HHCPA78; THIF; VDUP1; thioredoxin binding protein 2; upregulated by 1; 25-dihydroxyvitamin D-3; TXNIP elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of human TXNIP. No significant cross-reactivity or interference between human TXNIP and analogues was observed.
• Sequence Length: 391

• Samples: Serum, plasma, tissue homogenates
• Assay Type: Quantitative Sandwich
• Detection Range: 15.6 pg/ml-1000 pg/ml
• Sensitivity: < 3.9 pg/ml
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
• Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
• Preparation and Storage: Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for TXNIP elisa kit

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for TXNIP has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any TXNIP present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TXNIP is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TXNIP bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 171184421
• NCBI GeneID: 10628
• NCBI Accession #: NP_006463.3
• NCBI GenBank Nucleotide #: NM_006472.4
• UniProt Accession #: Q9H3M7; Q16226; Q6PML0; Q9BXG9; B4E3D3
• Molecular Weight: 43,661 Da
• NCBI Official Full Name: thioredoxin-interacting protein
• NCBI Official Synonym Full Names: thioredoxin interacting protein
• NCBI Official Symbol: TXNIP
• NCBI Official Synonym Symbols: THIF; VDUP1; HHCPA78; EST01027
• NCBI Protein Information: thioredoxin-interacting protein; thioredoxin binding protein 2; thioredoxin-binding protein 2; vitamin D3 up-regulated protein 1; upregulated by 1,25-dihydroxyvitamin D-3
• UniProt Protein Name: Thioredoxin-interacting protein
• Protein Family: Thioredoxin-interacting protein
• UniProt Gene Name: TXNIP
• UniProt Synonym Gene Names: VDUP1
• UniProt Entry Name: TXNIP_HUMAN

Uniprot Description

TXNIP: May act as an oxidative stress mediator by inhibiting thioredoxin activity or by limiting its bioavailability. Interacts with COPS5 and restores COPS5-induced suppression of CDKN1B stability, blocking the COPS5-mediated translocation of CDKN1B from the nucleus to the cytoplasm. Functions as a transcriptional repressor, possibly by acting as a bridge molecule between transcription factors and corepressor complexes, and over- expression will induce G0/G1 cell cycle arrest. Required for the maturation of natural killer cells. Acts as a suppressor of tumor cell growth. Belongs to the arrestin family.

Protein type: Tumor suppressor; Mitochondrial; Apoptosis; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 1q21.1

Cellular Component: cytoplasm; mitochondrial intermembrane space; cytosol; nucleus

Molecular Function: enzyme inhibitor activity; protein binding; ubiquitin protein ligase binding

Biological Process: response to drug; transcription, DNA-dependent; positive regulation of apoptosis; platelet-derived growth factor receptor signaling pathway; negative regulation of cell division; negative regulation of transcription from RNA polymerase II promoter; cell cycle; response to estradiol stimulus; regulation of cell proliferation; keratinocyte differentiation; response to hydrogen peroxide; response to mechanical stimulus; protein import into nucleus; negative regulation of catalytic activity; response to glucose stimulus; innate immune response; response to progesterone stimulus; response to calcium ion